生体内テレフタール酸の分析 (第1報)

過塩素酸による沈殿法

抄録

When fowl or mammals were fed with a diet containing terephthalic acid and tetracycline-type antibiotics, the level of the latter in blood was more elevated and more potentiated than those fed with the acid free diet. Terephthalic acid however, has not been approved for use since a practical assay procedure for it has not been established.
Semimicrodetermination of terephthalic acid in biological materials was examined and this method was found to be suitable for its assay in urine. The procedure is as follows:
One to 5ml. of urine is taken into a test tube and mixed with an equal amount of 20% perchloric acid. The mixture is shaken well and stood for 15 minutes. The supernatant is centrifuged (3, 500r.p.m) for 5 minutes, the precipitate is added to 10ml. of 10% perchloric acid, shaken well, stood for 10 minutes and centrifuged (3, 500r.p.m. 5min.). The precipitate is extracted with 10ml. of ethanol for 10 minutes and finally centrifuged (3, 500r.p.m.). The re-extraction is necessary.
The absorbance of the ethanolic extract is measured at 240mμ and its value is converted to the content of terephthalic acid by the following equation.
terephthalic acid content (mg./ml. of sample)=E₂₄₀/ml. of sample (treated)-E₂₄₀/ml. of sample (control)/110