アンスロンによる糖類の呈色反応(第4報) : ケトースの特異呈色反応

抄録

According to Waldi's description, ketoses on TLC chromatogram, are coloured yellow by spraying anthrone-acetic acid-phosphoric acid reagent and heating them at about 110° for 5-6 minutes. The authors found that this colour reaction was not specific for ketoses as shown in Table I. When the chromatogram was heated at 60° for 10-25 minutes after spraying the same reagent, yellow colour was developed from fructose and sucrose, but no colour appeared from rhamnose, aldopentoses and aldohexoses. Phosphoric and acetic acid were essential for this reagent. Replacing glacial acetic acid by acetic anhydrid, the colour changed brownish yellow, but the limit of detection was not changed. The limit of detection is as follows : Fructose 1 μg; sucrose 5 μg. This reaction can be used for PPC chromatogram also.