1969 年 89 巻 3 号 p. 297-301
A spectrophotometric study was made on the interaction of the carcinogen 4-nitroquinoline 1-oxide (4-NQO) with nicotinamide, and formation of a charge-transfer complex, in which n-electron of the ring nitrogen of nicotinamide is transferred tothe π-system of 4-NQO was presumed from the following results : (1) A new absorption band characteristic of chargetransfer complex was exhibited in the vicinity of 402 mμ in the difference spectra of aqueous mixtures of nicotinamide and 4-NQO vs. 4-NQO. (2) Analysis of the new absorption band through the Benesi-Hildebrand's equation revealed that a complex of 1 : 1 molar ratio was formed. (3) Intensity of the absorption band decreased in acid region below pH corresponding to pKa of nicotinamide. (4) Intensity of absorption band, which was produced around 396 mμ in the difference spectra of mixed system of 1-methylnicotinamide and 4-NQO was not infiuenced by variation of pH. (5) In the case of mixed system of nicotinamide and 4 -nitropyridine 1-oxide, whose electron affinity is smaller than that of 4-NQO, difference band appeared at about 340 mμ, i.e., in the shorter wave length region than that of the NA-4-NQO system. The magnitudes of ε, K, ΔH, and ΔS values were consistent with the above presumption. Examination of solvent effect revealed that the new absorption bands arose from n→π* transition.