1970 年 90 巻 3 号 p. 378-383
When sulpyrins is administered to the rat, unchanged sulpyrin, and 4-monomethyl-aminoantipyrine (MAA), 4-aminoantipyrine (AA), and 4-acetylaminoantipyrine (AcAA), which are the metabolites, are detected in the urine. This study was undertaken to determine these excretions by colorimetric method, after being separated by paper chromatography. The determinations of sulpyrin, MAA, AA, and AcAA by colorimetric method are as follows. Sulpyrin and MAA are extracted from the paper with pH 5.9 buffer solution, colored by phosphomolybdic acid, and their absorbances are measured at 810mμ. AA and AcAA are extracted with 0.5N HCl, colored by α-naphthol, and their absorbances are measured at 490mμ. It was found that each of the substances in the standard mixed solution containg sulpyrin, MAA, AA, and AcAA (150μg/0.1ml each) showed a high recovery rate and reproducibility. Especially, the stability of sulpyrin increased markedly, and its value was indicated correctly. These excretions in the rat urine after administration of sulpyrin were separated clearly by the present method. Effective results were obtained even in a small amount of substances in the rat urine.