薄層クロマトグラフィーを用いる生薬成分の定量法に関する研究(第2報) : 薄層クロマトグラフィーを用いるカンゾウ中のGlycyrrhizinの定量法の研究

抄録

A new method for the determination of glycyrrhizin in Liquorice root by spectrophotometric measurements has been deviced, after its separation from the other constituents by TLC. 0.05 gram of powdered Liquorice root was extracted with 50% (v/v) ethanol, which was chromatographed on Kieselgel plate using as developer a mixture of butanol-3N NH₄OH-ethanol (=5 : 2 : 1). (Fig.1). The g1ycyrrhizin portion was scratched out of the chromatogram and extracted with 50% (v/v) ethanol. The absorption was measured through the filter 252 mμ and the glycyrrhizin content was determined according to the calibration curve (Fig.2) previously prepared. This manipu1ation took about 12 hours and the recovery rate was found to be more than 95% in average (Table II). Considering that the separation of g1ycyrrhizin from the other accessary components is complete, this method can be considered to be superior to the previously described ones.