Abstract
Gas chromatographic separation of the derivatives of β-hydroxyalkanoic acids ranging from C6 to C18 was investigated. Separation into each member of the trimethylsiloxy-trimethylsilyl esters, the trimethylsiloxy-methyl esters, or the hydroxy-methyl esters was attempted by using a series of commercially available stationary phases with a hydrogen flame ionization detector system. As methyl esters, α-and β-hydroxyalkanoic and n-alkanoic acids were well separated from each other on a 3% HI-EFF 8B column. Further, a mixture of methyl β-hydroxyalkanoates ranging from C6 to C18 was successfully separated by a programmed temperature gas chromatography.
