クロモトロープ酸を用いるカリクレインのエステロリティック活性の測定法

抄録

The colorimetric determination of methanol with chromotropic acid was applied for assaying the activity of kallikreins to hydrolyze p-toluenesulfonyl-L-arginine methyl ester (TAME). This method was found to be useful for a fine determination of the specific activity in terms of μmoles of TAME hydrolyzed per minute for a certain amount of sample. The assay results from this method were in good agreement with those from the usual hydroxamate method. This method was more simple in manipulation, and was found to be 10 times more sensitive than the hydroxamate method.