In continuation of the preceding work, content of free amino acids in 28 kinds of domestic lichens was determined by the amino acid autoanalyzer (Hitachi Model KLA-2) and total nitrogen by the semimicro-Kjeldahl method. The lichens examined in the present series contained from 11 to 17 kinds of amino acid. In a total of 103 kinds of lichens examined in the previous (75) and present (28) series, content of alanine and glutamic acid was generally larger than that of other amino acids. The lichens that contained more than 100 μmoles of amino acid per 100 g of the lichem were ca.40% of the previous 75 kinds and ca. 61% in the present 28 kinds in the case of alanine, and ca.17.3% of the previous 75 kinds and co.25% of the present 28 kinds in the case of glutamic acid. As in the previous work, taurine was found in Cetraria endocrocea (ASAHINA) SATO, Cladonia bellidiiflora (ACH.) SCHAER, Cladonia granulans VAIN., and Ramalina crassa (DEL. ex NYL.) MOT. The total nitrogen of 1.88-2.70% in 3 kinds of Lobaria in Stictaceae and of 2.64% in one kind of Pannaria in Pannariaceae was 1argeer than that in other lichens. A large amount of total nitrogen was also found in 4 kinds of Lobaria and 1 kind of Pannaria in the previous work. In most of the 28 kinds of lichens examined in the present work, some Ninhydrin-positive substances other than amino acids constituting the protein were detected but no detailed examination was made on them.
