総合感冒剤中の主成分の定量法に関する研究(第10報)塩酸トリペレナミンのシアン化臭素による螢光定量法と反応機構について

抄録

Fluorometric determination of tripelennamine hydrochloride (I) in mixed anti-cold pharmaceutical preparations was studied, and a selective assay method with high sensitivity was established, The fluorescent substance of this reaction was extracted and isolated as an iodide. This substance, mp 150-151°, has the formula C₁4H₁5N₂I, and its chemical structure was found to be 1-benzyl-2, 3-dihydroimidazo1o[1, 2-α]pyridinium bromide, and the fluorescence is produced by ring formation to a quaternary ammonium compound, accompanied with separation of dimethylamino group, from the result of a moderate oxidation of I by BrCN. This assay method is as follows : Tripelennamine is extracted with petroleum ether from the sample solution containing about 4 mg of I and the extract is evaporated with small amount of citric acid. The residue is dissolved in EtOH to make 1000 ml. To 4 ml of this ethanolic solution, 10 ml of pH 8 buffer solution and 4 ml of 2.5% BrCN solution are added and the mixture is heated at 50° for 30 min. The fluorescence of cold solution is determined at 415 mμ, exciting at 340 mμ.