フェノール誘導体のけい光分析(第2報)アセトアミノフェンのけい光定量 その1

抄録

Fluorometric determination of acetaminophen (I) was studied and a recommended procedure was established. When I was oxidized with K₃Fe(CN)₆ in a slightly alkaline solution, a blue violet fluorescence developed and its intensity was increased by the addition of dimethylformamide. The fluorescent substance exhibited an absorption maximum at 303 mμ in ethanol which could not be observed in I, and it was considered that this absorption is closely related to fluorescence. Its chemical structure was found to be 2, 2'-dihydroxy-5, 5'-diacetylaminobiphenyl from its analytical data. The analytical procedure was as follows : To 1 ml of I solution (20 μg/ml), 2ml of 0.2 M H₃BO₃-KCl-Na₂CO₃ buffer solution (pH 8.5) was added, the mixture was cooled to 0°, and 1 ml of 0.05% K₃Fe(CN)₆ solution was added. After further standing for 5 min at 0°, 2 ml of 0.25% ascorbic acid solution and 5 ml of dimethylformamide were added and fluorescence intensity of the solution was measured at 425 nm, exciting at 337 nm.