1974 年 94 巻 5 号 p. 639-644
A fluorometric method was examined for the determination of acetamiophen (I) in pharmaceutical preparations. I was hydrolyzed with HCl to produce p-aminophenol (II) which was reacted with benzylamine (III) at 75°in alkaline solution to give a fluorescent substance. Based on these reactions, indirect fluorometric determination of I was established. II was also determined without any interference of I at 20° even if 1000 times its amount of I was added. The analytical procedure for I in pharmaceutical preparations was as follows : To 5 ml of 0.1 N NaOH solution containing 50 mg of I, 5 ml of HCl was added and the solution was boiled for 60 min. The solution was cooled to room temperature and diluted to 2 μg/ml with water. To 1 ml of this solution, 1 ml of 0.1 N NH4OH-NH4Cl buffer solution (pH 10.5) and 1 ml of 5% III-HCl solution (adjusted to pH 10.5 with 2 N KOH) were added, and the mixture was heated for 90 min at 75°. After cooling, 1 ml of 0.5 N KOH was added, and diluted to 20 ml with dimethylformamide. The fluorescence intensity of the solution was measured at 490 nm, exciting at 350 nm.