ゲンノショウコ成分の研究(第2報) Ellagitanninについてその1

抄録

The tannin of Geranium thunbergii was extracted with acetone/H₂O at room temperature, and the solution was extracted with EtOAc. This extract was fractionated using droplet countercurrent chromatography to yied tannin 1 and tannin 2. The relative astringency measurement and quantitative determination of ellagitannin by ultraviolet absorption of the solutions colored with NaNO₂-AcOH showed that the tannin activity of this plant is mainly due to the substances in the EtOAc extract in which tannin 1 and tannin 2 are the main constituents, and also that ellagitannins are the main constituents of the tannins in this plant. When treated with boiling water, tannin 1 was fairly rapidly hydrolyzed, and the resulting solution gave, upon fractionation after methylation, corilagin, hexahydroxydiphenoic acid, and gallic acid as their methylated derivatives. Glucose was also detected. An additional hydrolysis product, in which another galloyl group was presumed to be linked to corilagin, was obtained by treatment with boiling water for a short period. Tannin 1, therefore, would be one of the main original substances which yielded previously reported corilagin and other compounds related to tannin, which were obtained from the hot water extract.