抗炎症薬に関する研究(第29報)Tinoridineのラット肝Lysosome酵素の遊離および脂質過酸化に対する抑制作用

抄録

Both the release of enzymes and the lipid peroxidation were induced by the addition of hydrogen peroxide to the lysosome suspension prepared from rat liver homogenate. In this experimental model with hydrogen peroxide, Tinoridine inhibited the lipid peroxidation more strongly than the release of enzymes, at concentrations from 1.0 to 100 μM. At a high concentration of 1000 μM, Tinoridine did increase the release of enzymes, but markedly inhibited the lipid peroxidation. Moreover, Tinoridine inhibited the spontaneous release of enzymes from lysosomes by incubation without hydrogen peroxide. Tinoridine was also found to inhibit the lipid peroxidation in the 9000×g supernatant fraction of rat liver homogenate by incubation with or without hydrogen peroxide at concentrations from 1.0 to 1000 μM. These properties of Tinoridine seem to be different from those of acetylsalicylic acid, prednisolone, or α-tocopherol. These evidences suggest that Tinoridine has not only a membrane-stabilizing activity on lysosomes but also an inhibitory activity against the lipid peroxidation.