1976 年 96 巻 6 号 p. 777-782
Determination of 22 amino acids, including asparagine and glutamine, in serum by gas liquid chromatography (GLC) of N-isobutyloxycarbonyl (N-isoBOC) methyl ester derivatives was carried out. Serum samples were deproteinized with picric acid and purified with a cation-exchange resin. Amino acids thus obtained were derived to theif N-isoBOC methyl esters and analyzed by GLC. Recoveries of amino acids added to sera ranged 93.1-100.5% ; an exception was tyrosine which gave 70.3%. The data obtained by GLC method were in good agreement with those by the automated amino acid ana1yzer.