YAKUGAKU ZASSHI
Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
Streptomyces sp.の菌体から抗炎症成分としての脂肪酸類の分離と同定
三村 務大西 昇豊田 繁武藤 徳男青沼 繁
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1977 年 97 巻 11 号 p. 1240-1245

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Erythrocyte membrane stabilizing fraction (EMSF) was purified from the acetone extract of the mycelium of Streptomyces sp. F4818 by the combination of chromatography on silica gel and preparative thin-layer chromatography on silica gel. 1) Heat-induced hemolysis was significantly inhibited by EMSF at a concentration of 2 μg/ml and the hypotonic hemolysis was also inhibited by about 50% at a concentration of 12 μg/ml of EMSF. 2) EMSF showed high anti-inflammatory activity for carrageenin-induced edema in the hind paw of rats at the oral dose of 50 mg/kg. 3) It was proved that EMSF was a fatty acid mixture, consisting of C14 : 0, iso- or anteiso-C14 : 0, C15 : 0, iso C15 : 0, C16 : 0, iso-C16 : 0, iso- or anteiso-C17 : 0, C18 : 0, and C18 : 1 from the result of gas chromatography-mass spectra (GC-MS) analysis on their methyl esters. 4) Palmitic acid, the main fatty acid component in EMSF, markedly suppressed the heat-induced, hypotonic hemolysis in vitro and carrageenin-induced edema in vivo.

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© by the PHARMACEUTICAL SOCIETY OF JAPAN
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