Separation and Quantitative Analysis of Diterpene Alkaloids in Japanese Aconitum Roots

Abstract

A satisfactory method for quantitative determination of a series of diterpene alkaloids in Japnaese aconite roots was developed, using the combination of dual wave length thinlayer chromatography scan and gas chromatography. As an application of this method, change of each diterpene alkaloid content in some Japanese Aconitum species was examined in various seasons.