YAKUGAKU ZASSHI
Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
Rapid Estimation of Angiotensin I-Converting Enzyme Activity by High-speed Liquid Chromatography
ATSUO NAGAMATSUSHINJI SOEDAJINICHI INOKUCHI
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1978 Volume 98 Issue 9 Pages 1296-1299

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Abstract

Activity of angiotensin I-converting enzyme [EC 3.4.15.1] was assayed by chromatographical separation of the synthetic substrate (Bz-Gly-Gly-Gly or Bz-Gly-His-Leu) and the cleaved product (Bz-Gly) using high-speed liquid chromatography (HLC). Bz-Gly from each unhydrolyzed substrate was separated on the column of Zipax SAX (50 cm×2.1 mm i.d.), and with 0.01 M potassium phosphate buffer (pH 6.27) as eluant. In this condition, 1.3×10-4 to 2.4×10-3 units of the enzyme activity was measurable when Bz-Gly-Gly-Gly was used as the substrate, and 3.3×10-4 to 6.0×10-3 units of the activity was assayed with Bz-Gly-His-Leu as the substrate.

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© by the PHARMACEUTICAL SOCIETY OF JAPAN
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