p-Phenylenediamineのラットにおける生体内分布, 排泄および代謝

抄録

The distribution, excretion and metabolism of p-phenylenediamine, a constituent of oxidative hair dye, was studied after intraperitoneal administration of p-phenylenediamine-¹⁴C (1.5 mg/kg) to male rats. In tissues other than the small and large intestines, the highest concentration of radioactivity was found at 1 hr, followed by a rapid decrease in concentration. Only small amounts of radioactivity were found in the tissues 48 hr after administration. The low levels of radioactivity in tissues, together with the large amounts of radioactivity found in the intestinal tract and in urine, indicated the rapid excretion of administered p-phenylenediamine. Within 24 hr after administration, 69% of the administered radioactivity was excreted in the urine, 29% in the feces, and 26% of the intravenously administered radioactivity was excreted in the bile. Thin-layer chromatography of the urine indicated the presence of several urinary metabolites. The reverse isotope dilution method identified one of the metabolites as N, N'-diacetyl-p-phenylenediamine, which accounted for 30% of the radioactivity in the urine.