Loop-mediated isothermal amplification for rapid identification of Mycobacterium tuberculosis in comparison with immunochromatographic SD Bioline MPT64 Rapid® in a high burden setting

Benjawan Phetsuksiri; Janisara Rudeeaneksin; Sopa Srisungngam; Supranee Bunchoo; Wiphat Klayut; Somchai Sangkitporn; Chie Nakajima; Shigeyugi Hamada; Yasuhiko Suzuki

doi:10.7883/yoken.JJID.2018.128

This article has now been updated. Please use the final version.

Loop-mediated isothermal amplification for rapid identification of Mycobacterium tuberculosis in comparison with immunochromatographic SD Bioline MPT64 Rapid^® in a high burden setting

Benjawan Phetsuksiri, Janisara Rudeeaneksin, Sopa Srisungngam, Supranee Bunchoo, Wiphat Klayut, Somchai Sangkitporn, Chie Nakajima, Shigeyugi Hamada, Yasuhiko Suzuki

Author information

Benjawan Phetsuksiri
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
Janisara Rudeeaneksin
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
Sopa Srisungngam
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
Supranee Bunchoo
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
Wiphat Klayut
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
Somchai Sangkitporn
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
Chie Nakajima
Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control
Shigeyugi Hamada
Research Collaboration Center for Emerging and Re-emerging Diseases, Osaka University
Yasuhiko Suzuki
Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control

Keywords: tuberculosis, identification, LAMP, immunochromatography

JOURNAL FREE ACCESS Advance online publication

Article ID: JJID.2018.128

DOI https://doi.org/10.7883/yoken.JJID.2018.128

The final version of this article is now available: Vol. 72 (2019), No. 2 pp. 112-114

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Abstract

Loop-mediated isothermal amplification (LAMP) was assessed for rapid identification of Mycobacterium tuberculosis complex (MTC) in comparison with an immunochromatographic test (ICT) using SD Bioline Ag MPT64 Rapid^®. One hundred and fifty-one MGIT cultures positive for acid-fast bacilli (AFB) were tested for MTC. DNA was extracted from a small portion of culture samples by heat lysis and subjected to LAMP analysis. Of these, 1445 were positive and 5 were negative by both tests. One culture that was ICT negative but that was LAMP positive was confirmed to have a mutation in the mpt64 gene. The agreement was 98.68% [95% confidence interval (CI) 94.80-99.77] and the kappa value 0.83% (95%CI (0.59-1.00), respectively. The good correlation result suggested that LAMP assay was a reliable molecular test for rapid identification of MTC. It is practical for use in resource-limited, high burden settings.

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