Japanese Journal of Infectious Diseases
Online ISSN : 1884-2836
Print ISSN : 1344-6304
ISSN-L : 1344-6304
Development of a universal and lineage-specific primer sets for Zika virus (ZIKV) rapid detection in blood and urine samples by using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Thu Thuy BuiMeng Ling MoiKouichi MoritaFutoshi Hasebe
キーワード: Zika, RT-LAMP, blood, urine
ジャーナル フリー 早期公開

論文ID: JJID.2019.073


Zika is a mosquito-borne disease that is causing significant public health threats in recent years. Zika virus (ZIKV), the causative agent of this disease, is classified into two distinct genetic lineages: Asian and African lineages. While molecular nucleic acid methods have been proved useful for the diagnosis of ZIKV infection, development of assays based on one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) offers advantages including shorter incubation times, ease of handling and rapid detection. In this study, a universal LAMP primer set was developed to target conserved sequence of known ZIKV lineages. Additionally, Af7462 and As1788 primer sets were designed based on LAMP-based SNPs typing for the specific detection of African and Asian lineages. The RT-LAMP assays detected specifically African and Asian lineages, with the limit of detection range from 0.17 FFU/ml – 2.3x102 FFU/ml. As ZIKV viremia ranges between 102 to 106 PFU/ml or 103–106 copies/mL, the data indicate that the viremia range of clinical samples is within our detection range. Because of the high specificity and sensitivity and ease of use, the results suggest the utility of the assay in early clinical diagnosis applications.

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