Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
IN VITRO AGGREGATION OF PLATELETS INDUCED BY α-TOXIN (PHOSPHOLIPASE C) OF CLOSTRIDIUM PERFRINGENS
TAKAO SUGAHARATOMOKO TAKAHASHISHUN-ICHI YAMAYAAKIRA OHSAKA
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1976 Volume 29 Issue 5 Pages 255-263

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Abstract

Highly purified α-toxin (phospholipase C) of Clostridium per-fringens prepared by affinity chromatography on agarose-linked egg-yolk lipoprotein induced the in vitro aggregation of platelets of an irreversible type. The aggregation started after a time lag, the length of which depended on the concentration of the toxin; the reciprocal of the time lag was found to be directly proportional to the toxin concentration.
Using this assay method, we demonstrated that the platelet-aggregating activity of α-toxin reached minimum at around 70 C but heating at higher temperatures inactivated it to a lesser extent; the same anomaly in heat inactivation was observed with phospholipase C activity possessed by the toxin. By subjecting purified α-toxin to isoelectric focusing, four molecular forms were isolated, all of which were associated with both the platelet-aggregating and phospholipase C activities.
From all these results we concluded that the entity responsible for the platelet-aggregating activity is identical with α-toxin (phospholipase C) .

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