新規微生物酵素の開発と有機合成への利用

抄録

New enzymes screened for specific conversions have been purified and characterized from various microorganisms, and successfully applied to organic synthesis. These include : 1) nitrile hydratase from Arthrobacter sp. and Pseudomonas chlororaphis, useful in the hydration of nitriles to produce amides, 2) NAD⁺-dependent phenylalanine dehydrogenase from Sporosarcina ureae and Bacillus sphaericus, useful in the reductive amination of phenylpyruvate to give L-phenylalanine, 3) D-aminpeptidase from Achromobacter sp. applicable to the kinetic resolution of amino acid amides to give D-amino acid, and the D-stereospecific aminolysis reaction in organic solvents of amino acid esters or amides to yield D-amino acid N-alkylamides.