1998 Volume 56 Issue 11 Pages 931-939
A new way of solubilizing hydrolytic enzymes in organic media was achieved by coating the enzyme with lipid monolayers. Lipid-coated enzymes have been used successfully for a number of types of reactions in organic media or organic-aqueous two-phases, for example, enantioselective esterification by lipase, transphosphatidylation of water-insoluble phospholipids by phospholipase D, hydrolysis of lipophilic substrates by a catalytic antibody, and transglycosylation by β-D-galactosidase.