有機合成化学協会誌
Online ISSN : 1883-6526
Print ISSN : 0037-9980
ISSN-L : 0037-9980
合成化学と生薬学との接点-天然活性化合物に対するモノクローナル抗体と生合成酵素
正山 征洋
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ジャーナル フリー

1999 年 57 巻 8 号 p. 708-719

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MAb against THCA was produced by fusing hybridoma with splenocytes immunized with THCA-BSA conjugate and mouse myeloma cell. The cross-reaction of anti-THCA MAb against other marihuana compounds was very wide. This ELISA was applied to the biotransformation experiments of marihuana compounds using plant tissue culture.
Anti-ginsenoside Rb1 MAb cross-reacted with only limited to a few ginsenosides. Newly developed Western blotting used anti-ginsenoside Rb1 MAb surveyed only dammaran saponins. Immunoaffinity column made it possible to separate ginsenoside Rbl directly. Compact MAb, scFV against forskolin was prepared from a hybridoma by gene construction, and its expression in E. coli was discussed.
Biosynthetic enzymes, THCA-, CBCA- and CBDA-synthase had been purified from the homogenate of fresh Cannabis plants, respectively. These characterizations were investigated and compared each other. It became evident that CBCA-synthase catalyzed enantiomeric cyclization of CBGA to CBCA.

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