有機合成化学協会誌
Online ISSN : 1883-6526
Print ISSN : 0037-9980
ISSN-L : 0037-9980
有機合成におけるイソプレノイド生合成関連酵素 : 酵素機能改変と炭素-炭素結合形成ツールとしての有用性と展望
槙 雄二長岐 正彦西野 徳三古山 種俊
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2002 年 60 巻 8 号 p. 783-793

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Farnesyl diphosphate (FPP) synthase (FPS) catalyzes the condensation of isopentenyl diphos-phate (IPP) with allylic primer to give FPP as final product. The FPS from pig liver has been successfully applied to syntheses of bioactive compounds. Molecular cloning and expression of the gene for thermostable FPS from Bacillus stearothermophilus made it possible to produce sufficient amounts of the enzyme. However, it can hardly accept the substrate analogs having oxygen atom in their alkyl chain, which are easily accepted by pig liver enzyme. We constructed many mutated FPP synthases (FPSs) from B. stearothermophilus, in which Tyr-81 was substituted with Ser (S), Arg (R), Asp (D), or Gly (G). Interestingly, the substrate specificities of the mutated enzymes have been dramatically altered. They can easily accept the substrate analogs having oxygen atom. These results indicate further possibilities in application of the mutated enzymes to organic syn-thesis.

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