The purpose of this study is to investigate pulp-irritation and the direct pulp-capping effect of adhesives.
Twenty-one teeth of 9 patients were subjected to this stufy. Under local anesthesia, each vital pulp of the tooth was exposed in diameter from between 1.2-2.2mm (m=1.7mm).
They were pre-treated with 10% NaOCl Gel for 1-5min and chemical surgery. Direct capping with Ca (OH)
2 was undertaken in the control 4 teeth, and they were restored with Clearfil Photo Posterior (Kuraray). In the experimental group of 17 teeth, direct capping was undertaken on 10 teeth with Liner Bond System (Kuraray), 3 teeth with Super Bond C&B, Ivory (Sunmedical). Restored tooth were extracted after 37-194days (m= 79days), they were preserved for 2 months in 10% neutral buffered formaline solution and decalcified Serial paraffin sections were made and stained with Hamatoxylin-Eosin double stain and Van Gieson stain. Bacteria infection was examined by the Hucker-Conn stain method.
Clinical unpleasant symptoms such as slight discomfort and cold water reaction were observed in 11 teeth (52%) immediately after the treatment, but they disappeared completely soon. Clinical complaints were not observed in 10 teeth (48%) which could functionate immediately after the treatment. After 3-4 weeks, medium range complaints such as discomfort and intermittent spontaneous pain appeared in 2 teeth (10%) which had been capped with Ca (OH)
2. Histopathologically, most of the pulp tissues were normal. Light inflammatory cell infiltration was observed in 3 teeth (14%). Collagen fiber network formation were observed in 18 teeth (86%), and dentin bridge formation was identified in 20 teeth (95%). Bacterial invasion was observed in 18 teeth (86%). None of the bacteria was identified in 3 teeth (14%), but these were of a short experimental duration, that is, only 37-38days. Adhesives did not give irritate pulp or it was extremely low. When pulp is protected by capping and restoration with an adhesive, collagen matrix and new dentine were formed on the exposed surface of the pulp and this should extend to the complete dentine bridge in the maturation process.
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