All issues
Volume 1 (1966)
- Issue 4 Pages 157-
- Issue 3 Pages 107-
- Issue 2 Pages 53-
- Issue 1 Pages 1-
Volume 1, Issue 2
Displaying 1-8 of 8 articles from this issue
- |<
- <
- 1
- >
- >|
-
Masatoshi KOBAYASHI, Masayoshi YAMAZAKI1966 Volume 1 Issue 2 Pages 53-60
Published: June 25, 1966
Released on J-STAGE: February 07, 2008
JOURNAL FREE ACCESSThis paper deals with both the procedure of extraction and the chemical characteristics of the proteinic brain hormone from pupal brains of the silkworm. Acetone dried brains were extracted with phosphate buffer solution and the extract was lyophilized. The 60 μg crude extract yielded positive result on Bombyx-bioassay. The chemical properties of the active extract were examined with heat treatment at 60 or 100°C, buffer treatment at pH 9.9 or 1.4, digestive procedure with some proteolytic enzymes, and fractional precipitation by ammonium sulfate, all results showing that the extract belonged to proteinic substance. Then, the active fraction was separated from the crude extract by means of chromatographic studies with Sephadex and cellulose ion exchanger. When 1 μg of the fraction-V obtained by CM-cellulose column was injected into each of test-objects, the latter became an imago in 3 weeks following the injection. Moreover, the highly purified, active ingredient was separated from the fraction-V on CM-cellulose by means of the zone electrophoresis on acrylamide gel, showing that the ingredient migrated to the anode. Thus two different consituents in the brain extract, sterol and protein showed brain hormone activity.View full abstractDownload PDF (690K) -
Takashi MITSUI, Takao SAGAWA1966 Volume 1 Issue 2 Pages 61-68
Published: June 25, 1966
Released on J-STAGE: February 07, 2008
JOURNAL FREE ACCESSIn order to study the relationship between the queen differentiation and nucleotides, acid-soluble nucleotides of royal jelly, which was taken from queen cells at different stages, and worker jelly of honey bee, Apis mellifera L. were analysed by Dowex-1 (formate form) ion exchange column chromatography. The fractions, separated by this procedure, were identified with paper chromatography, paper electrophoresis and chemical analysis. The differences were not found in quality but in quantity of each sample. The rate of base composition of total nucleotides was approximately same in each sample, about 50 per cent adenosine, 30 per cent uridine, 5 to 7 per cent guanosine and small amount of cytidine nucleotides. The total contents of nucleotides were found to be highest in 48 EJ. UDP and UDP-sugar type nucleotides were found in large amount in royal jelly which was taken from early stage of queen cells.View full abstractDownload PDF (663K) -
Masatsugu FUKAYA, Socho NASU1966 Volume 1 Issue 2 Pages 69-72
Published: June 25, 1966
Released on J-STAGE: February 07, 2008
JOURNAL FREE ACCESSA new iridescent virus obtained from two larvae of the rice stem borer, Chilo suppressalis WALKER, was described and named Chilo iridescent virus (CIV). It seemed to be the first finding of this kind of virus in a lepidopterous insect collected in nature. The virus particle appeared hexagonal in outline and the average diameter was about 160 mμ. Although it showed morphological similarity to TIV, little serological relationship between CIV and TIV was so far observed. The lavae of the rice stem borer were readily infected with CIV by intrahemocoelic inoculation or by feeding.View full abstractDownload PDF (909K) -
Michihiko SAKAI1966 Volume 1 Issue 2 Pages 73-82
Published: June 25, 1966
Released on J-STAGE: February 07, 2008
JOURNAL FREE ACCESSThe cholinesterase (ChE) inhibition by nereistoxin hydrogen oxalate in adult male housefly, Musca domestica L., was investigated to reveal the role of the inhibitory action in the insecticidal action of this compound. The injected nereistoxin (100μg/g) caused only a slight depression of ChE activity in the fly head. The experiments in vitro using the fly-head homogenate as a ChE preparation showed nereistoxin was a week, competitive, reversible and unprogressive inhibitor of ChE. The known amount of acetylcholine in the insect nervous tissue, the active dosage of nereistoxin in its insecticidal action and the results of the present experiments indicated that the anti-ChE activity of this compound did not play the fundamental role in the insecticidal action. However, it was estimated that high dosages, more than 200μg/g, exerted 50 per cent inhibition of the ChE activity. Therefore the anti-ChE action by such a high dosage might be able to play an additional effect in the toxic action.View full abstractDownload PDF (839K) -
Katsuo KANEHISA1966 Volume 1 Issue 2 Pages 83-93
Published: June 25, 1966
Released on J-STAGE: February 07, 2008
JOURNAL FREE ACCESSThis experiment was undertaken to see effects of cholinesters, some bioactive amines, amino acids, several autonomic nerve agents and some miscellaneous compounds on the isolated cockroach hindgut movement. Cholinesters produced rather mild effect than other agents, they tend to increase the coordination, frequency and amplitude of the hindgut movement, but did not induce the contraction of hindgut. Acetylcholine had high effectiveness and its threshold of effective concentration was 10-8 g/ml. Tryptamine analogues produced increases of contraction, amplitude and frequency. Contraction of hindgut was a specific action by these agents, and this contraction seemed to be good for hindgut movement. Serotonin had the highest effectiveness among all tested compounds, its threshold of effective concentration was 10-11 g/ml. Catechol and phenyl amines showed very high increases of amplitude and frequency in a short while after application, however these stimulatory actions were followed by progressive decreases of amplitude and frequency. Glutamic acid and glutamine had high stimulatory action among amino acids, their threshold of effective concentrations were about 10-8 g/ml, many other amino acids had little or no effect on the hindgut movement. The present studies involved discussion of possible receptor system.View full abstractDownload PDF (1591K) -
Nobuhiko HOKYO, Keizi KIRITANI, Fusao NAKASUJI, Masakazu SHIGA1966 Volume 1 Issue 2 Pages 94-102
Published: June 25, 1966
Released on J-STAGE: February 07, 2008
JOURNAL FREE ACCESSThe general bionomics of the principal egg parasites of the southern green stink bug, Nezara viridula L., namely Asolcus mitsukurii ASHMEAD and Telenomus nakagawai WATANABE, were studied. A. mitsukurii differs considerably from T. nakagawai in the following points. 1) A. mitsukurii is polyphagous and prefers the pentatomids which deposit eggs in a small egg mass, while T. nakagawai is oligophagous and prefers large egg mass of pentatomids such as N. viridula or N. antennata. 2) A. mitsukurii is bisexual and the first egg deposited by a mated female was always a male, but T. nakagawai is unisexual producing female progeny by parthenogenesis. 3) Both sexes of A. mitsukurii have aggressive behavior, but T. nakagawai is not aggressive. 4) The developmental period of the female A. mitsukurii at 25°C was shorter by about 3 days than that of T. nakagawai, but the female of the latter was more prolific and lived longer than the former. These specific properties were discussed in comparison with those of other egg parasites of Nezara.View full abstractDownload PDF (805K) -
Jun MITSUHASHI1966 Volume 1 Issue 2 Pages 103-104
Published: June 25, 1966
Released on J-STAGE: February 07, 2008
JOURNAL FREE ACCESSDownload PDF (542K) -
Narumi YOSHITAKE1966 Volume 1 Issue 2 Pages 104-106
Published: June 25, 1966
Released on J-STAGE: February 07, 2008
JOURNAL FREE ACCESSDownload PDF (558K)
- |<
- <
- 1
- >
- >|