A gas chromatographic method for the determination of respiratory quotient was described. The system employed two columns in series, the filled with Porapak Q[○!R] separated CO
2 from air, and the second packed with molecular sieve 5A separated O
2 from N
2. Each gas component was measured with a thermal conductivity detector; slight differences between the concentrations were detected and the reproducibility was good. Effects of the O
2 concentration on the respiration of a wolf spider, Pardosa astrigera (L. KOCH), were investigated. Reduction in the O
2 concentration to 17-18% signifcantly depressed the respiration. The results suggest that the O
2 concentration inside the respiratory chambers should not decrease below this critical value.
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