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Volume 1 (1968)
- Issue 4 Pages 217-
- Issue 3 Pages 147-
- Issue 2 Pages 63-
- Issue 1 Pages 1-
Volume 1, Issue 1
Displaying 1-6 of 6 articles from this issue
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HIDEO TAKAMATSU, KEI-ICHI HIRAI1968 Volume 1 Issue 1 Pages 1-10
Published: 1968
Released on J-STAGE: March 04, 2010
JOURNAL FREE ACCESSThe stable Nadi reaction of myeloid leucocyte granules has been reported to be a non-enzymatic reaction because of the stability against fixation and heating for 10min at 100°C. Dimethyl-p-phenylenediamine and α-naphthol solutions are oxidized spontaneously, and a combination of these solutions gives a fine Nadi reaction. Freshly prepared regents are not so effective. Therefore, the chemical mechanism of Nadi reaction seems to be based on a dehydrative condensation of the two reagents.
The present study demonstrated that some naphthoquinone may be a Nadi reaction factor in the granules of leucocytes obtained from the peritoneal cavity of rat by peptone stumulation. It was proved that the reaction in these granules was affected by acetone. Although about 10% of leucocytes showed a positive reaction to treatment with pure acetone for 2hr (probably eosinophiles), all of the reactivity was lost when the treatment was continued 24hr longer. But the reaction was restored by vitamin K. However, vitamin K had no effect when leucocytes were treated with 96% acetone. This experiment suggests the presence of a naphthoquinone-like substance, which may be bound to phospholipids.
A clear liquid substance of high viscosity was extracted from leucocytes with 100% acetone. This was not soluble in alkali or water but was soluble in ethanol, chloroform and acid, and slightly soluble in methanol. This liquid became faint yellowish brown in color when exposed to light. In ultraviolet spectrophotometry, this acetone-extracted substance showed fine absorption peaks at 256 and 325mμ. This absorption spectrum corresponds with that of naphthoquinones, i. e. vitamin K.View full abstractDownload PDF (14114K) -
KOMEI NAKAO, ALFRED ANGRIST1968 Volume 1 Issue 1 Pages 11-15
Published: 1968
Released on J-STAGE: March 04, 2010
JOURNAL FREE ACCESS1) A highly sensitive aliphatic aldehyde reacting reagent, (3-methyl-2-benzothiazolone hydrazone, “Sawicki reagent”) was applied to connectivetissue histochemically, particularly for collagen and elastin. 2) Nomarked constant difference in the staining of lathyritic elastica or collagenwas noted. 3) The striations of striated muscle showed a faintly positivereaction. 4) All other elements (adipose tissue, muscles, skin appendages, epithelial cells, serum, etc.) appeared dusky purple in color.
Further studies at critical levels are necessary for the practical application of this biochemical procedure on tissue sections.View full abstractDownload PDF (7544K) -
TAKETOSHI SUGIYAMA1968 Volume 1 Issue 1 Pages 16-30
Published: 1968
Released on J-STAGE: March 04, 2010
JOURNAL FREE ACCESSOne of the nonspecific reactions of alcian blue is its inability to demonstrate the acid mucopolysaccharide electrostatically bound to proteins. A typical example is acid mucopolysaccharide present in the collagen bundles. This was demonstrated by treating the tissue with urea solution prior to fixation. The acid mucopolysaccharides in mast cell granules and in cartilage matrix and DNA of cell nuclei were also shown to be incompletely masked to larger basic dyes such as alcian blue. Smaller dyes had a better affinity to the polyanions in these tissue elements. By urea treatment, the alcianophilia of these materials was much enhanced. The precipitation study in the test tube showed that the negative charge of DNA was stronger than that of hyaluronic acid. From these facts, it was concluded that high histochemical specificity of alcian blue to acid mucopolysaccharide against DNA might be explained by two factors; by its large molecular size and by the presence of proteins which protect DNA from electrostatic combination with alcian blue in histological sections.View full abstractDownload PDF (21802K) -
YASUO KISHINO1968 Volume 1 Issue 1 Pages 31-36
Published: 1968
Released on J-STAGE: March 04, 2010
JOURNAL FREE ACCESSA histochemical method for the demonstration of glutamic oxalacetic transaminase was described, using a stabilized diazonium salt which coupled easily with oxalacetate produced by this enzyme reaction. Usefulness and specificity of the method were discussed in the present study. In addition, with the adaptation of this method, a study of the distribution of the transaminase activity in various tissues of rats was performed. High activities were demonstrated in heart muscle, striated skeletal muscle fibers, liver cells, cerebellar folia and proximal convoluted tubules of the kidney.View full abstractDownload PDF (9570K) -
KAZUAKI TAKADA, KOICHI TAMURA, MASAHIKO MORI1968 Volume 1 Issue 1 Pages 37-42
Published: 1968
Released on J-STAGE: March 04, 2010
JOURNAL FREE ACCESSThe effect of the presence of Mg++ and Zn++ upon the alkaline phosphatase activity of decalcified section was demonstrated in the following 4 groups histochemically.
1) Group 1. (non-treated): A weak enzymatic activity was found in articular and hypertrophic cartilage cells in epiphysis.
2) Group 2. (incubation medium containing Mg++ or Zn++ was used): A moderate enzymatic activity was found in articular and hypertrophic cartilage cells. The surface of primary bone trabeculae showed a strong stainability.
3) Group 3. (pretreated for a short time): The enzymatic activity was moderate to high at the surface of epiphysial plate.
4) Group 4. (pretreated for a long time): A strong enzymatic activity was found in the surface of epiphysial plate and of bone trabeculae. The surface of primary bone trabeculae was the strongest stainability.
The difference of staining intensity between using Mg++ and Zn++ as metallic ion could not be demonstrated. Alkaline phosphatase activity of the decalcified hard tissue was increased by the presense of Mg++ and Zn++ histochemically.View full abstractDownload PDF (7502K) -
KEI-ICHI HIRAI1968 Volume 1 Issue 1 Pages 43-55
Published: 1968
Released on J-STAGE: March 04, 2010
JOURNAL FREE ACCESSDuring the course of an investigation of the properties of 3, 3′, 4, 4′tetraaminobiphenyl (3, 3′-diaminobenzidine), microscopic observations demonstrated that an oxidized radical intermediater of tetraaminobiphenyl gives sensitive microbody staining in aldehyde-fixed rat liver cells when the tissue slices are incubated for 3 to 6hr in a saturated solution of tetraaminobiphenyl oxide, which was kept in air and light, at pH 7.2 without H2O2. The reagent was precipitated along the inner membranes of the mitochondrial cristae, in some cytoplasmic particles of leucocytes, and in erythrocytes as well as in the microbodies of hepatic cells. This reaction occurred anaerobically and even after dry heating for 30min at 120°C, but was inhibited by 10-3M KCN, 10-3M NaN3 or 10-4M Na2S2O4. Triazole in concentration of 10-2M inhibited the reaction of microbodies.
Spectrophotometric determination confirmed the presence of specific bonds between radical tetraaminobiphenyl oxide and heme enzymes, at least cytochrome c, catalase and hemoglobin. It is concluded that tetraaminobiphenyl oxide has an intense affinity for hemoprotein.
Catalase-rich microbodies are equally distributed in all the parenchymal cells, and mitochondrial hemoproteins are more abundant in the periportal than in the centrolobular areas.View full abstractDownload PDF (20262K)
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