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Volume 12, Issue 1
Displaying 1-10 of 10 articles from this issue
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S.L. LYUBSKII, I.B. BUCHWALOW, N.T. RAIKHLIN1979 Volume 12 Issue 1 Pages 1-6
Published: 1979
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSBy electron cytochemistry diffuse Ca-ATPase (pH 8.5) and 5′-nucleotidase activities were observed in the chromosomes of Chinese hamster fibroblasts. Ca-ATPase activity was stronger in the kinetochores than in the chromatin and was not found in cells at interphase, early prometaphase and under colchicine block. 5′-nucleotidase activity was localized in the nuclear chromatin of interphase cells and in the chromosomes of intact dividing and colchicineblocked cells.View full abstractDownload PDF (4208K) -
FUMIE SASAKI1979 Volume 12 Issue 1 Pages 7-19
Published: 1979
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSTail muscles of Rana catesbeiana and Xenopus laevis during metamorphosis were investigated ultracytochemically for lactate dehydrogenase (LDH). By means of the copper ferrocyanide method, LDH reaction products were found in the mitochondria, the muscle cell membrane, pinocytotic vesicle, T-tubules of vesicular triads and the sarcoplasm of the two types of muscle fibers (red and white fibers).
The localization of the LDH reaction products which are described and discussed, suggests a correlation with the histochemical and biochemical properties of the anuran tadpole tail during metamorphosis.View full abstractDownload PDF (8504K) -
IKUKO NAGATSU, SHINOBU INAGAKI, YUKARI KONDO, NOBUYUKI KARASAWA, TOSHI ...1979 Volume 12 Issue 1 Pages 20-37
Published: 1979
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDistributions of tyrosine hydroxylase (TH) and dopamine-β-hydroxylase (DBH) in the rat brain were examined using immunofluorescent staining on unperfused fresh frozen sections. TH-positive and DBH-negative dopaminergic neurons were observed in the anterior hypothalamic nucleus. In other hypothalamic nuclei such as ventromedialis, dorsomedialis, and posterior hypothalamic nuclei, a few dopaminergic neurons were also observed.
Our immunofluorescent results with unperfused fresh frozen sections indicated that TH or DBH was mainly localized in the cell bodies of catecholaminergic neurons and that small amounts of the enzymes were distributed in the nerve terminals. Although it had been reported that the amine enzymes existed in nerve terminals as well as in the cell bodies by using paraformaldehyde perfused sections, the fluorescence observed in nerve terminals might be produced not only by the amine enzymes but also by amines themselves.View full abstractDownload PDF (9555K) -
MORPHOLOGICAL CHANGES OF THE MUSCLES AND MOTOR END-PLATES OF CENTROPHENOXINETREATED AGED GUINEA PIGSYOKO UCHIDA, MASANORI TOMONAGA1979 Volume 12 Issue 1 Pages 38-46
Published: 1979
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSCentrophenoxine, 80 mg/kg/day, was administered to 25-month old guinea pigs intraperitoneally for 90 or 127 days and the muscles and motor end-plates were examined histochemically. The results showed some improvement of muscle atrophy and deformity of the motor end-plates in the aged guinea pigs. However, it is not clear whether these changes result from the central action or the peripheral action of centrophenoxine.View full abstractDownload PDF (3216K) -
SHOTARO HISAMITSU1979 Volume 12 Issue 1 Pages 47-55
Published: 1979
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSAn improved disc gel electrophoretic method for detecting glycogen phosphorylase isozymes is described. Gels free of glycogen primer were first electrophoresed to avoid different electrophoretic migrations of the enzymeprimer complex and then were preincubated for 2 hrs to adjust the optimal pH (pH 5.7). Finally they were post-incubated in substrate-primer mixture for phosphorylase reaction using iodine.
The electrophoretic bands of the isozymes could be more sharply differentiated by the present technique.View full abstractDownload PDF (4734K) -
SHIGEKI KUZUHARA, MASANORI TOMONAGA, YASUO TOYOKURA, TOSHIAKI TAKASU1979 Volume 12 Issue 1 Pages 56-62
Published: 1979
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSHistochemical characteristics of the external anal sphincter muscle (EAS) of the rabbit were compared with those of the extensor digitorum longus muscle (EDL) and soleus muscle (Sol).
EAS was composed of 0.2% type 1, 62.5% type 2A and 37.3% type 2B fibers, while EDL was composed of 7.6% type 1, 23.2% type 2A and 69.2% type 2B fibers, and Sol almost solely of type 1 fibers. Fiber diameters of each fiber type in EAS were from one half to two thirds those of corresponding ones in EDL or Sol.
In recent years combined studies on the physiological and histochemical properties of each fiber type have demonstrated that type 1 fibers are slow twitch and fatigue-resistant, type 2A fibers, fast-twitch and fatigue-resistant, and type 2B fibers, fast-twitch and fast-fatigue. EAS as well as EDL therefore composed of fast-twitch fibers, though the former is composed predominantly of type 2A fibers, and the latter predominantly of type 2B fibers. The predominance of type 2A fibers in EAS may be qualified for the unique function of keeping the anal canal closed to fecal urgency.View full abstractDownload PDF (3463K) -
TOSHIYUKI IWAMOTO, KIYOAKI YAMASHITA, SOICHI IIJIMA1979 Volume 12 Issue 1 Pages 63-68
Published: 1979
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSSpectrophotometric measurements were made on to quantitate batch variations of orcein dyes. Three types of spectra were observed among eleven batches tested. Four showed bimodal curves with a peak absorption at 520nm and smaller peak at 595nm (Type A). Five showed a single peak at 520 nm (Type B) and two synthetic orceins gave a single broad peak at 510 nm (Type C) Modified Shikata's orcein stain for hepatitis B surface antigen was tested with these reagents and large variations in staining capacities were noted. An immunoenzyme method was performed for confirmation and comparison of orcein reaction. Type A orcein gave the best results and staining specificity was found to increase in proportion to the amount of absorption at 595 nm. Results of staining with good batches of orcein were equally satisfactory with immuno-peroxidase staining. The latter method being expensive and timeconsuming, orcein staining was found to be method of choice and a spectrophotometric analysis may facilitate choice of the batches.View full abstractDownload PDF (2615K) -
ATSUSHI SUZUKI, HIDEO TAMATE1979 Volume 12 Issue 1 Pages 69-74
Published: 1979
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSThe M. pectoralis thoracicus, M. iliotibialis lateralis, and M. pubischiofemorales of the Japanese quail were histochemically investigated. The type A fibers with a high activity of NADH: Nito BT oxidoreductase (NADHOX) and alkali-stable myosin ATPase showed a relatively high activity of glycerol-3-phosphate: menadione oxidoreductase (GPOX) and a low to high activity of 3-hydroxybutyrate: NAD+ oxidoreductase (HBOX). The type B fibers with low NADH activity and high alkali-stable ATPase activity were moderate in GPOX activity and very low or absent in HBOX activity. The type C fibers with high NADHOX activity and low alkali-stable ATPase activity showed a low GPOX activity and a high HBOX activity. The pectoralis thoracicus contained numerous A fibers and few B fibers; the iliotibialis lateralis had few A fibers and many B fibers. The M. pubischiofemorales pars externus contained numerous A fibers and few B and C fibers, whereas the pars internus possessed few A fibers and many C fibers. It is suggested that the M. pubischiofemorales pars internus with many C fibers differs (in function) from the pars externus with few C fibers.View full abstractDownload PDF (2271K) -
HIDETOSHI FUKUNAGA, MITSUHIRO OSAME, MASARU KAWABUCHI, AKIHIRO IGATA1979 Volume 12 Issue 1 Pages 75-79
Published: 1979
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSCholinesterase staining and Gomori's trichrome staining were performed in serial sections of soleus muscles of rats daily administered with 0.625mg/kg neostigmine for 2 or 3 weeks. In the affected muscle fibers, abnormally expanded subsarcolemmal sarcoplasmic masses were frequently observed with a homogenous greenish blue appearance by Gomori's trichrome staining. Cholinesterase activities were clearly demonstrated in serial sections at the areas corresponding to these sarcoplasmic masses.
In teased fibers of the affected muscles, the areas adjacent to cholinesterase activities appeared enlarged and expanded.
It is evident that there exists a close relationship between the sarcoplasmic mass and the motor endplate histochemically.View full abstractDownload PDF (3955K) -
Akiko OKADA1979 Volume 12 Issue 1 Pages 80-93
Published: 1979
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSMy previous microscopic and autoradiographic observations revealed that the covering membrane of a rabbit's blastocyst was not simply the zona pellucida, and that the zona pellucida was soluble in mercaptoethanol, but the blastocyst membrane was insoluble in mercaptoethanol. In this study, the sulfur content of the zona pellucida and the blastocyst membrane was measured with an EPMA to determine if sulfur changes quantitatively in the egg at various stages of development. Experiments were performed with oviducal and uterine eggs from Japanese white rabbits, 15 hr to 6 days after ovulation, and follicular eggs. The eggs were fixed in glutaraldehyde and OsO4, dehydrated with alcohol, and dried on carbon or embedded in Epon. The sulfur analysis was performed with an EPMA (JEOL LTD, JMA-50A); the accelerating voltage was 15 kV and the specimen current was 0.03μA. A PET crystal was used for diffractive analysis. The sulfur value in the zona pellucida of the follicular eggs and in the uterine eggs were 25 and 75 counts/sec respectively. The amount of sulfur, which is considered a component of the mucoprotein in the blastocyst membrane, was significantly higher than in the zona pellucida.View full abstractDownload PDF (13688K)
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