巻号一覧
12 巻 (1979)
- 6 号 p. 483-
- 5 号 p. 409-
- 4 号 p. 283-
- 3 号 p. 197-
- 2 号 p. 97-
- 1 号 p. 1-
12 巻, 4 号
選択された号の論文の12件中1～12を表示しています
- |<
- <
- 1
- >
- >|
-
KANJI KASAI, YOSHINOBU YOSHIDA1979 年 12 巻 4 号 p. 283-291
発行日: 1979年
公開日: 2009/10/28
ジャーナル フリーThe present paper describes the production and secretion mechanisms of human chorionic gonadotropin (hCG). In order to determine more detailed mechanisms of production and secretion of hCG, a morphological study was done by ultrastructural, histochemical and immunohistological examinations using β-subunit of hCG (β-hCG), which is the determinant of both the biological and immunological activity of hCG.
Small pieces of human chorionic tissue were collected from pregnant women in an early stage of gestation (mainly in the seventh and eighth week) by curette or at hysterectomy. Human chorionic gonadotropin and its subunits are a kind of glycoprotein, which is represented by a saliva-insoluble PAS positive substance in syncytial trophoblast. The substance appears as secretion granules in electron microscopic study. Fine granules appear first in the perinuclear Golgi zone with a surrounding smooth dense membrane, and gradually increase in size toward the cell surface where liquefaction and liberation into the maternal blood occur.
Immunohistology reveals mostly the same process of production and secretion of granular β-hCG only in syncytial trophoblast.
Comparative morphology among electron microscopy, histochemical and immunohistological examinations lead us to the conclusion that hCG, which is produced only in the syncytial trophoblast, appears as granules in intra-cytoplasmic distribution as well as mechanisms of production and secretion.抄録全体を表示PDF形式でダウンロード (10332K) -
HIROO KIMOTO, TOMIO ODA1979 年 12 巻 4 号 p. 292-300
発行日: 1979年
公開日: 2009/10/28
ジャーナル フリーA new method of staining histamine in rat mast cell granules in formalin fixed paraffin section was developed. Mast cells of several organs of formalin fixed paraffin sections were stained well in red with orcein-water blue as well as in purple with toluidine blue. But only mast cells in dermis showed a different appearance in orcein-water blue stain and toluidine blue stain. The results of an electrophoretic study, diamine oxidase treatment and compound 48/80 treatment of rat lead to the conclusion that the substance stained in red with orcein may be histamine. It was shown that histamine in rat mast cell granule is fixed even by formalin, a finding contrary to previous studies.抄録全体を表示PDF形式でダウンロード (16206K) -
TAKENOBU KISHIDA, SHOZO KITO, EIKO ITOGA, NOBORU YANAIHARA, NORIO OGAW ...1979 年 12 巻 4 号 p. 301-324
発行日: 1979年
公開日: 2009/10/28
ジャーナル フリーIn recent years, the significance of biologically active polypeptides which are widely distributed has been stressed. The authors studied the distribution of substance P-like immunoreactivities (SPLI), somatostatin-like immunoreactivities (SLI) and met-enkephalin-like immunoreactivities (ELI) using the indirect immunofluorescence technique. The fiber and dot-like appearances of SPLI, SLI and ELI were observed in the dorsal horn, the anterior horn, the reticular formation, the lateral tegmental nucleus, the ventral thalamic nucleus, the mesencephalic central gray, the medial hypothalamus, the amygdaloid nucleus, the accumbens nucleus, the cerebral cortex and so on; namely, not only in the primary sensory system as already reported by others, but also in some parts of areas belonging to the extrapyramidal, the limbic and the hypothalamo-pituitary systems and in the cerebral cortex. The authors discussed functional correlations between these three biologically active polypeptides and between the peptides and monoamines.抄録全体を表示PDF形式でダウンロード (40295K) -
BUNSUKE OSOGOE, HAJIME MIYAMOTO1979 年 12 巻 4 号 p. 325-329
発行日: 1979年
公開日: 2009/10/28
ジャーナル フリーThe patterns of labeling of thymic lymphocytes and germinal-center cells of the rat with [5-3H] deoxycytidine (labeled in the 5-position) in vivo were studied by autoradiographic technique, and compared with the patterns of labeling with [G-3H] deoxycytidine (generally labeled).
With respect to the thymic lymphocytes, no significant difference was observed in labeling intensity between the two labeled markers. In the germinal-center cells, on the other hand, the labeling intensity with [5-3H]-deoxycytidine was found to be significantly weaker than [G-3H] deoxycytidine labeling. The results are discussed in relation to the ability of these cells to utilize deoxycytidine for the synthesis of DNA-thymine, and the pool size of thymidine within the tissue which suppresses such utilization of deoxycytidine by these cells. It was found that the thymus contained a large pool of thymidine, while in the lymph node the pool seemed much smaller in size.抄録全体を表示PDF形式でダウンロード (4624K) -
B. PRESENTEY, Z. JERUSHALMY1979 年 12 巻 4 号 p. 330-336
発行日: 1979年
公開日: 2009/10/28
ジャーナル フリーTwenty-four analytic-grade, purified or synthesized organic substances of various types and sources were subjected to 12 different methods of histochemical staining, most of them currently in use for identification of phospholipids by the spot-test technique. The aim of the study was to ascertain the degree of specificity of the various histochemical methods, particulary those intended for identifying phospholipids. None of the tested methods was found to be selectively specific for lipid or lipid-like substances, which suggests that the results of histochemical methods should be interpreted carefully and critically before drawing specific conclusions.抄録全体を表示PDF形式でダウンロード (2558K) -
KENICHIROU INOMATA, KAZUO OGAWA1979 年 12 巻 4 号 p. 337-343
発行日: 1979年
公開日: 2009/10/28
ジャーナル フリーThiamine monophosphatase activity in the substantia gelatinosa of rat spinal cord was studied under the electron microscope. The reaction products were chiefly localized on the plasma membrane of electron dense sinusoid axon terminals. The precipitates were occasionally localized on the plasma membrane of neighboring axons and dendrites. However, no thiamine monophosphatase activity was found in membrane-bound dense bodies, the Golgi apparatus, the associated so-called GERL system, and the endoplasmic reticulum of the substantia gelatinosa nerve cells.
It is postulated that acid phosphatase activity on the plasma membrane of electron dense sinusoid axon terminals in the substantia gelatinosa may have some function related to thiamine metabolism.抄録全体を表示PDF形式でダウンロード (10347K) -
TOMOYUKI HARADA1979 年 12 巻 4 号 p. 344-355
発行日: 1979年
公開日: 2009/10/28
ジャーナル フリーFor the purpose of clarifying the functional significance of clear- and compact-type cells, correlative hormonal and morphological studies were per-formed on adrenocortical tumors associated with primary aldosteronism, Cushing's syndrome or non-dyshormonal symptoms. The content of aldosterone, corticosterone and cortisol in adrenal tissue was estimated by radioimmunoassay, and, on the surface of a quantitative sample, the ratio of its constituent cell-type was examined in sections with oil red O stain and/or H-E stain.
The content of aldosterone and corticosterone was significantly higher in primary aldosteronism (P<0.001) than in Cushing's syndrome and in non-functioning tumor, with a mean value of 1.22±0.15 and 7.52±1.05ng/mg tissue, respectively. In Cushing's syndrome, cortisol content showed a high value, 9.27±1.60ng/mg tissue. The steroid content was different in each case, and varied with parts of the quantitative sample even in the same case.
Though the correlation of aldosterone content and compact-type cell population in primary aldosteronism showed a negative trend and that of cortisol content and compact-type cell in Cushing's syndrome had a positive, neither correlation was statistically significant.抄録全体を表示PDF形式でダウンロード (4877K) -
TAKESHI TSUCHIYA, HIDEO TAMATE1979 年 12 巻 4 号 p. 356-360
発行日: 1979年
公開日: 2009/10/28
ジャーナル フリーThe distribution of adenyl cyclase in sheep parathyroid gland was investigated using electron microscopy. The reaction products of the enzyme were located in the plasma membrane in intercellular spaces between the gland cells, confined exclusively to the external cell surface. The products were more abundant on the surface of the intricately interdigitated cells than on that of the relatively straighted cells. Secretory granules were mainly located near the plasma membrane which bordered the intercellular spaces. The functional significance of the enzyme localization in the parathyroid gland cells is discussed in relation to findings of other authors.抄録全体を表示PDF形式でダウンロード (4152K) -
SOTOKICHI MORII, TADAMI KUMAZAWA, TAKEO KUSUMOTO, HISATOSHI HARADA, AI ...1979 年 12 巻 4 号 p. 361-367
発行日: 1979年
公開日: 2009/10/28
ジャーナル フリーWistar SLC male rats were injected with 25mg/Kg of 7, 12-Dimethyl-benz(α)anthracene intravenously twice at 51 and 66 days of age, and also fed on a commercial diet containing 4-Dimethylaminostilbene, 0.004%, from the age of 57 days to the respective days of their decapitations. Fresh cryostat serial sections of Zymbal glands were examined by histochemical methods for SDH, LDH and G6PDH 6, 11 and 17 weeks after the first injection. Either atypical hyperplasia of the acinar cells or microtumor was detected in the Zymbal glands of almost all treated rats 11 weeks after the first injection. An ear duct tumor was induced in all treated animals 17 weeks after treatment. SDH and G6PDH activities decreased progressively in the tumorigenesis, but moderate LDH activity remained in the tumor tissues. Biochemical determinations for LDH and MDH in the Zymbal glands harvested serially 4-20 weeks after the first injection of DMBA indicated that the quotient of LDH/MDH began to elevate 8-11 weeks after injection and elevated in the tumorigenesis owing to a progressively lower MDH.抄録全体を表示PDF形式でダウンロード (4273K) -
TOSHIMITSU WATABIKI1979 年 12 巻 4 号 p. 368-382
発行日: 1979年
公開日: 2009/10/28
ジャーナル フリーThe localization of alcohol dehydrogenase (ADH) activity in the hepatic (parenchymal) cells of normal mice was studied ultracytochemically by both the copper ferrocyanide and the tetranitroblue tetrazolium (TNBT) methods.
Reaction products, copper ferrocyanide showing the enzymatic activity, were mainly found in the cytoplasmic matrix immediately adjacent to the rough-surfaced endoplasmic reticulum (rER). In addition, the reaction products were also observed on the inner and outer membranes of mitochondria, cristae mitochondriales, and in the rER.
The reaction was completely inhibited by pyrazole (20 to 60mM), but only partially by N-ethylmaleimide (30 to 60mM).
The results obtained by the TNBT method were not necessarily identical to those obtained by the copper ferrocyanide method and the probable causes of different findings obtained by the two methods are also discussed.抄録全体を表示PDF形式でダウンロード (26629K) -
KATSUKO KATAOKA, KEISUKE SHIMIZU, TOSHIHARU YAMAMOTO, JUNZO OCHI1979 年 12 巻 4 号 p. 383-390
発行日: 1979年
公開日: 2009/10/28
ジャーナル フリーMonoamine-containing cells and adrenergic nerve fibers are clearly demonstrated by fluorescence microscopy on semi-thin sections of Araldite-embedded materials previously fixed with a mixture of formaldehyde and glutaraldehyde. This enabled us to examine identical monoamine-containing structures by both fluorescence and electron microscopy.
Fluorescence microscopy reveals two kinds of monoamine-containing cells in the fundic mucosa of the kitten: one with yellow fluorescence and the other with blue-green fluorescence. Electron microscopically, yellow fluorescent cells are EC cells containing characteristic polymorphous secretory granules, while blue-green fluorescent cells have round secretory granules and are probably A, X and third type cells (Lausanne classification (9)).抄録全体を表示PDF形式でダウンロード (7664K) -
HISANOBU KAIYA, TSUYOSHI IWATA, MASUYUKI NAMBA, YASUHIRO AGO, HIROSHI ...1979 年 12 巻 4 号 p. 391-404
発行日: 1979年
公開日: 2009/10/28
ジャーナル フリーThe localization site of choline acetyltransferase (ChAc) activity in the neostriatum of the rat brain was ultracytochemically examined. Two of six neuronal types showed ChAc activity, which had previously been reported to be medium-sized interneurons with the Golgi method and an electron microscope study. All types of neurons except Cajal's dwarf cell in the neostriatum were revealed to receive a bouton containing ChAc positive synaptic vesicles and to form an axo-somatic synapse. This evidence was compatible with findings reported elsewhere that all types of neurons in the neostriatum synthesize acetylcholinesterase. A hypothetical schema of neuronal network concerned with neurotransmitters in the neostriatum was proposed.抄録全体を表示PDF形式でダウンロード (18020K)
- |<
- <
- 1
- >
- >|