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Volume 13 (1980)
- Issue 6 Pages 540-
- Issue 5 Pages 407-
- Issue 4 Pages 317-
- Issue 3 Pages 229-
- Issue 2 Pages 157-
- Issue 1 Pages 2-
Volume 13, Issue 4
Displaying 1-9 of 9 articles from this issue
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TADAO OKANO, AKIRA KAWAOI, TOSHIO SHIKATA, KAZUYOSHI DOBASHI, HAJIME O ...1980 Volume 13 Issue 4 Pages 317-323
Published: 1980
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSQualitative as well as quantitative study on antigenic stability of hCG and its subunits showed that fixatives tested were more or less injurious and that heating at 60°C or xylol treatment significantly affected the antigenecity. Fixation with 10% formalin or with Zamboni's solution had less effect. It was concluded that frozen sections were more suitable for detection of small amounts of hCG antigen than paraffin-embedded materials.View full abstractDownload PDF (3317K) -
HAJIME SUGIHARA, KANKATSU YUN, MARI HOTTA, HIDEO TSUCHIYAMA1980 Volume 13 Issue 4 Pages 324-329
Published: 1980
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSEffects of several conditions known to stimulate the zona glomerulosa of the adrenal cortex in the adult were examined on the fetal adrenal cortex of rats histochemically and electron microscopically. The stimulating conditions were the following; administration of Na restricted diet to the mother, administration of KCl or angiotensin II to the fetus, spironolactone or insulin to the mother. In these, only an intraperitoneal injection of KCl solution to the fetus affected the fetal zona glomerulosa, in which increased activity of glucose-6-phosphate dehydrogenase and hyperfunctional alteration of the mitochondria and the smooth endoplasmic reticulum were observed. This fact shows the fetal glomerulosa cells can react to orae of the specific stimuli on the adult glomerulosa cells.View full abstractDownload PDF (8385K) -
TOSHIHARU HAYASHI1980 Volume 13 Issue 4 Pages 330-342
Published: 1980
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSHistochemical studies were conducted on the carp (Cyprinus carpio) retina to reveal the cellular localization of catecholamines (CA) and acetylcholinesterase (AchE) activity. CA was found almost exclusively in interplexiform cells, which extend processes towards both the outer and inner plexiform layers, forming dense networks at the inner nuclear and plexiform layers. It was demonstrated that the endogenous CA is dopamine (DA) and that CA-cells are dopaminergic in nature. The AchE activity was seen in a certain group of amacrine cells and in some ganglion cells, and diffusely in the inner plexiform layer and weakly in external horizontal cells. Among cells located at the innermost row of the inner nuclear layer in radial sections counterstained with methyl green, approximately 1 and 35% were estimated to be DA-containing interplexiform cells and AchE positive amacrine cells, respectively. Simultaneous visualization of DA-fluorescence and AchE activity showed that AchE-positive processes are in contact with DA-fluorescent cell bodies.View full abstractDownload PDF (13500K) -
AYAO HIRASHITA, YOSHIKI NAKAMURA, EIJI OKUMURA, YOSUKE KUWABARA1980 Volume 13 Issue 4 Pages 343-358
Published: 1980
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSMolars of adult Wistar rats were moved experimentally by orthodontic elastic for four days. Then, energy dispersive X-ray microanalysis of mitochondrial granules in bone cells incidental to experimental tooth movement was performed. The following results were obtained. 1) In the unstained specimens treated with OsO4-glutaraldehyde, microanalysis seems to be possible for a portion of the elements. 2) In particular, a condition in which Ca may bind strongly with protein in mitochondria is shown. 3) At mineralizing sites in the experimental groups, Ca-K, P-K, and Os-M X-ray lines were detected in mitochondrial granules in morphologically active osteoblasts and young osteocytes, but we could not separate the P-K and Os-M peaks from their combined peak. At absorbing mineral sites, Ca-K was found markedly in the ruffled borders and vacuoles of osteoclasts. Ca-K X-ray line was detected also in mitochondrial granules of osteoclasts. 4) In the control groups, mitochondria of resting osteoblasts and osteocytes had few granules and the detection of Ca-K X-ray line was very hard from these mitochondria. Ca-K X-ray line was detected in the mitochondria close to the ruffled border of the osteoclasts, but it was not detected in the mitochondria located on the vascular side of the cells.View full abstractDownload PDF (22909K) -
ACID PHOSPHATASE AND THIAMINE MONOPHOSPHATASEKENICHIROU INOMATA, KAZUO OGAWA1980 Volume 13 Issue 4 Pages 359-367
Published: 1980
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSAcid phosphatase (AcPase) and thiamine monophosphatase (TMPase) activities from the dorsal root to the dorsal root ganglion neuron in the rat spinal cord were studied under the electron microscope. The reaction products for AcPase activity were localized on the axolemma of the dorsal root and in the lysosome, the Golgi saccules, the reticular part of the Golgi apparatus (so-called GERL) and a part of the endoplasmic reticulum of the dorsal root ganglion neuron. Other reaction products for TMPase activity were localized in the axolemma of the dorsal root and the Golgi saccules and the reticular part of the Golgi apparatus of the dorsal root ganglion neuron. Both higher activities for the dorsal root ganglion neuron were established by comparison with the other neurons. It is suggested here from investigation that both activities are transported from the dorsal root ganglion neuron to the synaptic membrane of the dense sinusoid axon terminal in the substantia gelatinosa.View full abstractDownload PDF (17405K) -
KATSUHIRO INOUE, CYRUS R. CREVELING1980 Volume 13 Issue 4 Pages 368-376
Published: 1980
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSCatechol-O-methyltransferase (COMT) was localized in the spleen, thymus, and lymph node of the rat using the unlabeled antibody enzyme technique. Immunoreactive deposits were found in the cells of the white pulp, marginal zone, and perinodular ring between the white pulp and marginal zone of the spleen and the sinus, germinal center and cortex of the lymph node. These cells appeared to be reticular cells and macrophages on the basis of their shape and distribution. COMT was present in epithelial reticular cells of the thymus. No COMT reaction product was found in bone marrow.View full abstractDownload PDF (12756K) -
G. P. VERMA, K. P. VERMA1980 Volume 13 Issue 4 Pages 377-385
Published: 1980
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSEmploying certain histochemical tests on the ovarian follicle of rabbit it has been found possible to conclude that the zone pellucida composed of PAS-positive sialomucin-containing non-sulphated acid mucopolysaccharide develops from the secretion of granulosa cells constituting zona radiata around the pre-antral oocyte.View full abstractDownload PDF (5834K) -
KEN FUJIMORI, KENSUKE CHIKAMORI, MASAYUKI SHONO, MASAAKI MIYAI, MASA-O ...1980 Volume 13 Issue 4 Pages 386-393
Published: 1980
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe TRIDENT, a specially devised microphotometer for dehydrogenase assay in tissue sections, was applied to examine the distribution of glutamic dehydrogenase (GDH) activity in tissue sections of the mouse brain. With this system, the activity, relative activity (RA), was represented by the color intensity of formazan due to enzymatic activity per tissue protein. The RA value obtained increased linearly with the incubation time up to 20min in various regions of the mouse brain. The GDH activity was quantitatively compared between four regions in the cerebellum, the cerebral cortex, hippocampus, substantia nigra and putamen in determining the RA per min. The cerebral cortex and hippocampus were relatively high in GDH activity. In the cerebellum, GDH activity of the granular layer was the highest of the four regions.
Thus, the TRIDENT was highly useful in quantitative examination of the discrete distribution of the enzyme activity of tissue sections.View full abstractDownload PDF (4108K) -
TOSHIO MASUZAWA, TAKUMA SAITO, FUMIAKI SATO1980 Volume 13 Issue 4 Pages 394-403
Published: 1980
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSTo determine the ultracytochemical localization of Na+, K+-ATPase activity on normal rat choroidal epithelium, K+ dependent-p-nitrophenylphosphatase activity was studied by using the one-step lead citrate method devised by Mayahara et al. (1978).
An electron dense reaction product was found to be abundant inside the microvilli and positively along the outside of the basal plasmalemmas of the choroidal epithelium. No reaction product was seen on the apical and lateral ones. In the cytoplasm, the electron dense precipitates were also observed in the endoplasmic reticulum and nuclear membrane. Ouabain (10-3-10-4M) effectively inhibited the activities in the microvilli and basal plasmalemmas. Alkaline phosphatase reaction stronger than that of Mg2+-ATPase was observed mainly on the outside of the basal plasmalemmas, but the activities of these two enzymes were not found in the microvilli.View full abstractDownload PDF (16841K)
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