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Volume 14 (1981)
- Issue 6 Pages 563-
- Issue 5 Pages 439-
- Issue 4 Pages 329-
- Issue 3 Pages 223-
- Issue 2 Pages 101-
- Issue 1 Pages 1-
Volume 14, Issue 5
Displaying 1-10 of 10 articles from this issue
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AIRO TSUBURA, SATOSHI UEDA, NOBUAKI SHIKATA, SOTOKICHI MORII1981 Volume 14 Issue 5 Pages 439-448
Published: 1981
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSGroups of pregnancy-dependent mammary tumors in GRS/A females, composed of maximally grown, slightly and fully regressed tumors, were observed by enzyme-histochemical methods, as compared with normal mammary glands of the same hosts. In such dependent tumors, activities of lactate, succinate and glucose-6-phosphate dehydrogenases and of acid and alkaline phosphatases were weaker than those in parenchymal cells of neighbouring functioning normal mammary glands. ACP was prominently detected in the necrotic foci within regressing tumors after parturition, while other enzyme activity disappeared there. Intensive activity of LDH was detected in some of the lining cells of microcysts within regressed tumors, where other enzymes decreased. In pregnancy-independent mammary tumors of this strain, activities of dehydrogenases except for LDH were much less than adjacent resting normal mammary glands, which seemed to demonstrate similar activities to those of dependent tumors. LDH was quite variable in independent tumors, but its activity became stronger in cancer cells. ALP distributed irregularly in some independent tumors.View full abstractDownload PDF (8897K) -
KOROKU NEGISHI, TSUNENOBU TERANISHI, SATORU KATO1981 Volume 14 Issue 5 Pages 449-460
Published: 1981
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSCarp (Cyprinus carpio) retinas were processed for fluorescence-microscopic studies with flat-mounts. When the fish were injected intravitreally with noradrenaline (NA; 20μg) 2hr before enucleation, the number of visible cells was approximately double that of endogenously fluorescent cells due to the additional appearance of smaller and weakly fluorescent cells. By injecting a mixture of NA (5μg) and 5, 6-dihydroxytryptamine (5, 6-DHT; 5μg) intravitreally 2hr prior to enucleation, simultaneous visualization of greenish and yellowish fluorescent cells was achieved. It was found that the former was larger in size and certainly belonged to a class of dopaminergic (DA-) cells, while the latter was smaller and corresponded to another class of cells, indoleamine-accumulating (IA-) cells. These findings were further confirmed by selective destruction of DA-or IA-cells with intravitreal injection of 6-hydroxydopamine (6-OHDA; 15μg) plus pargyline (15μg) or of 5, 7-dihydroxytryptamine (5, 7-DHT; 15μg) once daily on two succesive days, one week before enucleation. The density ratio of DA-cells to IA-cells was found to be 1:1, and they were similarly distributed in space in a mixed fashion. Their densities were higher in the peripheral region than in the rest, and extremely high at the retinal margin. Where the densities were higher, the size of cell bodies tended to be smaller. Those cells, located in the intermediate and peripheral regions, appeared to be arranged in radial rows originating at the optic disc and fanning out towards the retinal margin.View full abstractDownload PDF (7332K) -
AKINORI MIKI, YASURO ATOJI, HUMIO MIZOGUTI1981 Volume 14 Issue 5 Pages 461-475
Published: 1981
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSUsing a new method of performing 3H-thymidine autoradiography and histochemical demonstration of acetylcholinesterase (AChE) activity on the same sections, the relationship between the proliferating ability and AChE activity of the neural tube cells was studied in early chick embryos.
In 1.5-day embryos (stage 10), almost all the cells constituting the neural tube showed weak AChE activity, namely, the reaction products were detected along the nuclear membrane. Since these cells were labeled with 3H-thymidine they had proliferating ability. In 2-day embryos (stage 13), a few cells showing an intense reaction were first found near the basement membrane at the cervical level, in which the reaction products were demonstrated not only along the nuclear membrane but also in the cytoplasm. They were round in shape and not labeled with 3H-thymidine. They were regarded as neuroblasts. In addition to these cells, a few spindle-shaped cells having the reaction products along the nuclear membrane and in the cytoplasm were demonstrated in the ependymal layer (matrix). Up to four hr after the injection of 3H-thymidine, these cells were not labeled and after eight hr some of them were first labeled. This indicated that they were newly formed neuroblasts, having lost their proliferating ability.View full abstractDownload PDF (26169K) -
TOSHIMITSU WATABIKI, NORIAKI ISHIDA, KAZUO OGAWA1981 Volume 14 Issue 5 Pages 476-505
Published: 1981
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe effect of ethanol administration on alcohol dehydrogenase (ADH) activity in the hepatic parenchymal cells of ICR, C57BL/6J and DBA/2 mice was studied ultracytochemically by the copper ferrocyanide method. Also, the tetranitroblue tetrazolium (TNBT) method was partially used. The ethanoltreated mice were sacrificed either 6 (6hr group) or 30hr (30hr group) after the last administration.
ADH activity in all ethanol-treated mice, 6hr group was markedly increased in the cytoplasmic matrix immediately adjacent to both rough- as well as smooth-surfaced endoplasmic reticulum over that of the same ethanol-treated mice, 30hr group.
I. In 6hr and 30hr groups, ADH activity in the heavily dosed mice showed a significant decrease of the activity as compared to that of the moderately dosed mice. In long-term ethanol-treated mice, 30hr group, there was the maximum increase in ADH activity in both the cytoplasmic matrix and mitochondria at 15 and 18 months, after which the activity showed a significant decrease. In comparison with respective control mice, long-term ethanol-treated mice, 30hr group, showed slight increase in ADH activity. TNBT-formazans were positive in the cristae mitochondriales of long-term ethanol-treated mice, but not at all in the control mice.
II. In the 6hr, 30hr and control groups, ADH activity in C57BL mice increased over that of DBA mice.View full abstractDownload PDF (37210K) -
TAKESHI TSUCHIYA, HIDEO TAMATE1981 Volume 14 Issue 5 Pages 506-515
Published: 1981
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSParathyroid glands of seven species were studied by enzyme histochemical reactions for NADH-DH, SDH, α-GPD, β-HBD, ALPase, ACPase, and AChE. An intense reaction for NADH-DH was found only in the glands from rabbits while the others were strong to weak. A high intensity for α-GPD was found in the glands from sheep, pigs, and rabbits while those of the others did not show specificity with the enzyme reaction. The parenchymal cells of the glands of sheep and chickens reacted for ALPase, whereas those of the other species did only in the vascular walls. The ACPase reaction of the glands was intense in chickens, moderate in pigs, and weak in other species. The C-cells of the internal parathyroid glands within thyroid glands of rabbits reacted moderately for AChE, but the external glands of rabbits and those of the other species did negatively. The parathyroid glands of chickens were different from those of the other species in histochemical properties. Histochemically they showed the characteristics of hyperfunction demanded by increased Ca metabolism.View full abstractDownload PDF (13436K) -
R. C. RAJYALAKSHMI BHANU, K. SHYAMASUNDARI, K. HANUMANTHA RAO1981 Volume 14 Issue 5 Pages 516-523
Published: 1981
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA histological study of the fore-gut gland revealed the presence of clubshaped epithelium. Two types of cells, the secretory and absorptive could be recognized in the digestive gland epithelium.
It is evident from the histochemical tests that these glands are loaded with rich quantities of carbohydrates, basic protein, protein bound amino groups, sulfhydryls and moderate quantities of amino acids like tryptophan and tyrosine, glycoprotein and the enzymes, acid and alkaline phosphatases.View full abstractDownload PDF (8521K) -
MITSUAKI SUZUKI, HIROYUKI KURAMOTO, SHINICHI IZUMI, HIDEO SHIRANE, KEI ...1981 Volume 14 Issue 5 Pages 524-533
Published: 1981
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe changes of the endometrial alkaline phosphatase (ALP) along with menstrual cycles were investigated by both enzyme histochemistry and biochemistry. In order to avoid biassed views which may come mostly from insufficient observation on the limited number of cases, the endometrial specimens were obtained from over 180 patients without known abnormalities. Very fine cyclic changes of the ALP, including changes of the intracellular localization pattern, were clearly evidenced by histochemical observations, which were intimately substantiated by biochemical assay. In the proliferative phase, the ALP activity gradually increased to reach its peak at the early secretory phase, right after ovulation. In the mid to late secretory phase, a rather abrupt decrease of the enzyme was evidenced by an inverse increase of the mucin secretion which could be observed by alcian blue staining. On the basis of this clear cyclic change of the endometrial ALP, the mode of hormonal regulation, either by estrogen or by progesterone, on the enzyme activity was discussed.View full abstractDownload PDF (16082K) -
TATSUO SUGANUMA, SHINTARO SUZUKI, SHINICHIRO TSUYAMA, FUSAYOSHI MURATA1981 Volume 14 Issue 5 Pages 534-547
Published: 1981
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSDistribution of mucosubstances in the rat gastric mucosa was studied by radioautography using 35S-sodium sulfate, several light microscopic histochemical procedures, the periodic acid thiocarbohydrazide silver proteinate (PA-TCH-SP) method and postembedding staining with Iectin (wheat germ agglutinin) ferritin conjugates. Histochemical staining revealed that the superficial covering epithelium contained a large amount of neutral mucosubstances, whereas the foveolar and isthmus mucous cells produced both sialomucins and sulfomucins. Active incorporation of 35S was observed in the fundic foveolus and isthmus as well as the pyloric gland. The mucous neck cells contained sialomucin. The PA-TCH-SP method stained the covering epithelium, the epithelium of the foveolar and isthmus mucous cells and the mucous neck cells. All cell organellae which took part in the formation of granule substances in the gastric mucosa were stained by this method. The cytoplasm of the mucous neck cells was extensively occupied with round PA-TCH-SP positive granules. As the epithelium of the pits matured, the granules increased in size. The apical portion of the covering epithelium was exclusively occupied with large round to oval granules. Many of them showed a bipartite structure after PA-TCH-SP staining, reaction products were observed only in the peripheral region of these granules. Conversely, the development of other cell organellae became less prominent as these epithelia matured. Postembedding staining with lectin (wheat germ agglutinin)-ferritin conjugates stained secretory granules of all these mucous cells with different staining patterns. In the covering epithelium, the conjugate stained only the peripheral region of the granules.View full abstractDownload PDF (24237K) -
DENNIS MOE, SVEND KIRKEBY1981 Volume 14 Issue 5 Pages 549-553
Published: 1981
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSIn this paper the effects of disaccharides on α-naphthyl esterase from mouse kidney are investigated with histochemical, spectrophotometrical and electrophoretical methods. While a strong inhibition could be observed in the histological sections the biochemical measurements showed very little inactivation. The difference between the histochemical and biochemical results might be due to the difference in tissue preparation before measurement of the enzyme activity.View full abstractDownload PDF (4860K) -
KOH KAWAGOE, TAKASHI KAWANA, SHOICHI SAKAMOTO1981 Volume 14 Issue 5 Pages 554-560
Published: 1981
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSIn order to characterize the negative surface charge of the abnormal human trophoblast and to cast light on its biological role, the negative charges on the trophoblastic surface of spontaneous abortion, partial mole and complete mole were investigated by electron microscopy using cationized ferritin as an ultrastructural marker. The degree and distribution of negative charge were also studied in comparison with surface ultrastructure and surface glycoprotein identified by ruthenium red staining.
In a case of spontaneous abortion, the surface structure of the human trophoblast differed from site to site, correlating best with the degree of degeneration of the syncytiotrophoblast itself. For villi from a spontaneous abortion, which were otherwise normal in routine electron microscopy, the surface glycoprotein was observed to make prominent aggregates on the trophoblastic surface in concomitance with the reduced negative surface charge. In a severely degenerated system, the basic structure of the surface glycoprotein was lost in accordance with significant decrease of the negative surface charge. This finding seems to show the possible role of the negative surface charge of the trophoblast, that with the electrostatic repulsive forces, the membrane-bound glycoproteins can maintain their normal regular positions and, as a result, membrane specificity of the trophoblast can be expressed. When both complete hydatidiform mole and partial mole were observed by electron microscopy using ruthenium red staining and cationized ferritin, the complete mole differed essentially from the partial mole in that the former resembled normal villi while the latter resembled spontaneous abortion.View full abstractDownload PDF (7816K)
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