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Volume 16 (1983)
- Issue 6 Pages 567-
- Issue 5 Pages 445-
- Issue 4 Pages 293-
- Issue 3 Pages 175-
- Issue 2 Pages 77-
- Issue 1 Pages 1-
Volume 16, Issue 5
Displaying 1-11 of 11 articles from this issue
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AKINORI MIKI, HUMIO MIZOGUTI1983 Volume 16 Issue 5 Pages 445-455
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSAppearance and distribution of acetylcholine receptor (AChR) in the myotome cells of the chick embryo were investigated by autoradiography using 3H-α-bungarotoxin (α-BGT). Acetylcholinesterase (AChE) histochemistry was also performed on the same sections.
In the 1, 5 day-old embryo (H-H stage 11), the myotome was not formed and the specific labeling of the toxin was not found on any tissue components. In the 2 day-old embryo (stage 13), when the myotome first formed in the dorsomedial angle of the dermatome, some silver grains were demonstrated on the AChE-positive cells constituting the myotome, and thereafter, the grains on the myotome increased in number as well as in density, as development progressed. On the other hand, the specific labeling of the toxin was not observed on the neural tube, sclerotome (mesenchyme), dermatome or spinal nerves.
These findings indicate that since the AChE-positive myotome cells are no longer capable of proliferation (26), AChR appears in the myotome cells shortly after their final cell division.View full abstractDownload PDF (5081K) -
SHINICHIRO TSUYAMA, SHINTARO SUZUKI, FUSAYOSHI MURATA1983 Volume 16 Issue 5 Pages 456-471
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSThe histochemical feature of epithelial mucins of the rat proximal colon differed from that of the distals. The regional differences were elucidated by various mucin-staining methods, such as periodic acid-Schiff (PAS), Alcian blue (pH 2.5) -PAS (AB-PAS), high iron diamine (HID) -AB (pH 2.5), and O-acylated sialic acid specific staining: periodic acid-thionin Schiff (PATS) /-periodate-borohydride technique (PBT) /potassium hydroxide (KOH) /periodic acid-Schiff (PAS) technique for light microscopy, periodic acid (PA) -thiocarbo-hydrazide (TCH) -silver proteinate (SP) staining, O-acylated sialic acid specific staining and dialyzed iron staining for electron microscopy. The distribution of sugar residues of the glycoproteins in the colonic epithelial mucin was also examined by lectin-horseradish peroxidase (HRP) staining method. Goblet cells at the upper half of the crypt in the proximal colon contained mainly neutral mucin and at the lower half, their mucin showed alcianophilic nature predominantly. Sulfomucin secreting-cells were distributing only at the near luminal surface of the proximal colon and all through the crypt of the distal colon. Some goblet cells, columnar cells and vacuolated cells contained O-acylated sialoglycoconjugates. The lectin staining-patterns differed from each other. Some lectins stained the upper half goblet cell-mucin only or the lower half goblet cell-mucin, while other lectins stained goblet cells of the whole crypt. It seemed that the histochemical staining features changed at the branching site of the crypt in the proximal colon. On the other hand, such a transitional zone was obscure in the distal colon.View full abstractDownload PDF (8897K) -
FRANCESCO AMENTA, CARLO CAVALLOTTI, MARCO DE ROSSI, FRANCESCO VATRELLA1983 Volume 16 Issue 5 Pages 472-482
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSThe distribution pattern of quinacrine-positive, catecholamine-containing and acetylcholinesterase-positive nerve fibers was investigated in the rat brachial artery and vein using the quinacrine histofluorescence, the formal-dehyde-induced fluorescence and the acetylcholinesterase histochemical methods, respectively.
Some animals were chemically sympathectomized using the neurotoxin 6-hydroxydopamine in order to ascertain the nature of the different kinds of nerve fibers visualized. The density of innervation between artery and vein as well as between the various portions of single blood vessels was studied using quantitative image analysis.
No quinacrine-positive nerve fibers were found within rat brachial artery and vein. On the contrary both blood vessels were supplied by adrenergic and acetylcholinesterase-containing (likely cholinergic) nerve fibers. The pre-treatment of samples with quinacrine did not alter the intensity of the reaction neither the distribution pattern of nerve fibers, revealed with other histo-chemical techniques.
The quantitative image analysis showed that the brachial artery is provided with an adrenergic innervation, richer of about 30% than corresponding vein. In the artery the adrenergic innervation was more developed of the cholinergic one of about 20% and in the vein of about 15%. The artery is provided with a cholinergic innervation richer than corresponding vein of about 25%. The density of nerve fibers decreases gradually proceeding towards the distal ends of studied blood vessels.View full abstractDownload PDF (4632K) -
RYOJI NAITO, YOSHIAKI TAKAI, KOUJI TSUKITANI, KENICHI ASANO, MASAHIKO ...1983 Volume 16 Issue 5 Pages 483-493
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSHistochemical differences in complex carbohydrates in GCT and duct cells in SMGs of mice and rats were reported by use of lectins which link to specific sugar residues. Also, the relationship between carbohydrate-peptide complexes in the GCT and the growth factors, EGF, and NGF, were discussed. The granules in the GCT cells of the mouse SMG gave positive binding of PNA, and RCA to a moderate degree, and the staining intensity was a little higher in females than in males. Complex carbohydrates in GCT cells are suggested to contain galactose and N-acetyl-galactosamine residues. Binding of SBA, RCA, and WGA were low to moderate in the rat GCT when the peroxidaseconjugated lectin method was used. PA/Con A-HRP method in GCT cells showed intense staining. The biological properties of granules in rat GCT cells were different from those of mouse GCT cells.View full abstractDownload PDF (7949K) -
NORIYUKI SAHARA, KATSUHIKO FUKASAWA, MINORU HARADA, KAZUO SUZUKI1983 Volume 16 Issue 5 Pages 494-501
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSImmunohistochemical localization of dipeptidyl peptidase (DPP) IV in rat digestive organs was studied using the peroxidase-antiperoxidase PAP method.
In the rat digestive organ observed, a fairly intense immunoreaction of DPP IV was found on the brush border of enterocytes of the small intestine. However, the immunostaining of enterocytes in the small intestine showed marked regional differences. The intensity of the reaction was significantly higher in the jejunum and ileum than in the duodenum. DPP IV was also detected in the cytoplasm of Paneth cells in the jejunum and ileum and on the surface of the crypt epithelium of the colon. In the esophagus, a faint reaction was detected on the surface of the stratified epithelium and connective tissues of the submucosa. DPP IV could not be found in the cells of the stomach by immunohistochemical means.
The present results suggest that DPP IV in digestive organs could mainly participate in the final digestion of peptides in which a proline residue is involved.View full abstractDownload PDF (4358K) -
SOTOKICHI MORII, AIRO TSUBURA, YOSHIHIKO KOMAI, NOBUAKI SHIKATA, HARUA ...1983 Volume 16 Issue 5 Pages 502-512
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSAlpha-lactalbumin (LA) was isolated and purified from fresh human milk and injected into rabbits for antiserum production. By a PAP technique, LA was demonstrated in the cytoplasm of secretory (26 positive cases out of 26 examined cases) and resting (11/43) normal mammary glandular cells from operated human breasts. Some epithelial cells in the cases of mammary dysplasia (53/229), fibroadenoma (15/94) and carcinoma (10/183), and of gynecomastia (1/12) showed positivity. Histochemical staining features, reticular, granular and compact, corresponded to the secretory state of the cells. The diffuse and sporadic distribution of LA-positive cells within the mammary lobules and foci would suggest the character of biological alterations. Moreover, LA secretion may be affected by the menstrual status of patients. LA has never been detected in normal parenchymal cells of the skin, stomach, colon, liver, pancreas, lung, testis, ovary, uterus, placenta, umbilical cord, thyroid or salivary gland. The immunohistochemical examination for LA, therefore, has no significant role as a tissue marker in histopathologic diagnosis of breast cancer, but it could serve as a specific marker for the mammary gland, especially its differentiation.View full abstractDownload PDF (4532K) -
TOYOSHI FUJIMOTO, KAZUO OGAWA1983 Volume 16 Issue 5 Pages 513-521
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSFrog urinary bladders were fixed in a distended position and filipin was added to (A) both serosal and mucosal sides, (B) only serosal side, or (C) only mucosal side of the bladder, or to (D) scraped mucosal cells. Incubation was carried out from 30 min to 18 hr at room temperature.
In (A), filipin-sterol complexes were found in the apical cell membrane (AM) and the apical granule membrane (GM), but not in the basolateral cell membrane (BLM) at 30 min of incubation. On the contrary, in (B) at 15 hr, the complexes were found in BLM and GM, but not in AM. In (C) at 15 hr and in (D) at 2 hr, the complexes were found all in AM, GM and BLM. When the penetration of filipin seemed marginal in (A) and (B), ridges of 17 nm width on the P face and corresponding ditches on the E face were seen in GM and some protuberances and pits were found at the crossings of ridges and ditches.
The present results showed clearly that filipin penetrates very slowly and the filipin-sterol complexes may be formed nonuniformly even in adjacent membranes. The ridges and ditches might show a preliminary stage of the complex formation in GM.View full abstractDownload PDF (6064K) -
ADAM ANTHONY, NEIL W. BRISTER, GLORIA J. COLURSO1983 Volume 16 Issue 5 Pages 522-535
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSThe practicability of a recently developed cytochemical bioassay of corticotropin was evaluated with respect to potential sources of error associated with tissue preparative procedures, staining and microdensitometric analysis.
Careful and rigorous control of culture conditions prior to exposing cryostat sections is required to insure maintenance of adrenal tissue viability and responsiveness to ACTH. Maintenance of a constant pH of 7.6 and use of freshly prepared culture medium is especially critical. Instrumental error associated with microdensitometry constitutes a relatively minor source of error (1-2%). Also, intrasection variability (ca. 1-2%) does not appreciably influence the accuracy of ACTH bioassay. However, substantial error can stem from intersection variation (3-6%) in intensity of staining which presumably reflects nonuniformity in the thickness of serially sectioned adrenal glands.
The precision and accuracy of the cytochemical ACTH bioassay compares favorably with previously reported bioassays and with radioimmunoassay procedures for ACTH. The assay is much more sensitive than most existing bioassays since it can detect ACTH levels as low as 5-10 femtograms.View full abstractDownload PDF (2214K) -
MASAHIKO MORI, KENICHI HAMADA, RYOJI NAITO, KOUJI TSUKITANI, KENICHI A ...1983 Volume 16 Issue 5 Pages 536-548
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSHistochemically detected distribution of epidermal growth factor (EGF) in submandibular glands (SMG) of the mouse, rat, hamster, and guinea pig is reported using horseradish peroxidase-labeled antibody method. The following main points were established:
1. EGF in the SMG was confined to the granular convoluted tubule (GCT) cells and duct system.
2. EGF reaction in the GCT was most intense in the male mouse.
3. Sexual dimorphism of EGF distribution was very evident in mouse SMG, and somewhat so in rat and hamster glands, and not apparent in guinea pig glands.
4. EGF reaction in mouse SMG increased during pregnancy and was high at 1 and 2 weeks after delivery.
5. EGF in the postnatal stage of male mouse first developed at 3 weeks after birth.View full abstractDownload PDF (2377K) -
TOYOSHI FUJIMOTO, KAZUO OGAWA1983 Volume 16 Issue 5 Pages 549-558
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSIn the presence of glutaraldehyde (GLA), horseradish peroxidase (HRP) was found to bind to the basolateral cell membrane, but not to the apical membrane, of dissociated frog urothelial cells. To clarify the mechanism of the binding, the effect of various modifications to the reaction was studied.
The binding of HRP, i. e. “the HRP-GLA staining or reaction”, revealed the following characteristics: 1) The staining was observed only in the cells dissociated with cation chelation or enzyme treatment, and not in the cells scraped mechanically. 2) GLA should coexist in the same solution with HRP. Paraformaldehyde (FA) or dimethyl suberimidate (DMS) could not replace GLA. 3) Prefixation with GLA, FA or DMS could prevent the staining. 4) Diazotization of amino groups by prolonged sodium nitrite incubation abolished the reaction totally. 5) Pretreatment of cell with poly-L-lysine caused the apical membrane to show positive staining.
These results indicate that the HRP-GLA reaction stains the amino groups, which are exposed only after some dissociating treatments, and can be used as a cell membrane marker at an electron microscopic level.View full abstractDownload PDF (4280K) -
AKIRA IKEDA, NOBORU MISHIMA, RYO KAWANO, TANEKAZU HARADA1983 Volume 16 Issue 5 Pages 559-563
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (2359K)
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