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Volume 17, Issue 1
Displaying 1-8 of 8 articles from this issue
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MASAHIRO SAKAI, KAZUO OGAWA1984 Volume 17 Issue 1 Pages 1-13
Published: 1984
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe relation of cytoskeletal elements to the lysosomal wrapping mechanism (LWM) was investigated by using so-called tubulolytic agents (colchicine, vinblastine or nocodazole), a microfilament destabilizer (cytochalasin B) and a microfilament stabilizer (phalloidin) during autophagy in mouse subcutaneous histiocytes, which was induced by a subcutaneous injection of ovalbumin.
Pretreatment with the tubulolytic agents not only did not inhibit an occurrence of the LWM following ovalbumin injection, but induced the LWM even without ovalbumin injection. On the other hand, pretreatment with the destabilizer or the stabilizer of microfilaments prevented the occurrence of LWM induced by ovalbumin. The direct morphological connection between actin-like microfilaments and lysosomes performing the LWM was observed in histiocytes in specimens treated with sodium dodecyl sulfate or saponin. These results suggest that depolymerization or disorganization of microtubules induces or favors the occurrence of LWM, and that the presence of intact microfilaments, but not microtubules, is necessary for the occurrence of the LWM induced by ovalbumin.View full abstractDownload PDF (17988K) -
YUTAKA TSUTSUMI1984 Volume 17 Issue 1 Pages 15-21
Published: 1984
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSLeu 7 (HNK-1) antigen, a marker for lymphoid cells expressing natural killer (NK) activity, has recently been demonstrated by us in peripheral nerve fibers and normal and neoplastic neuroendocrine cells in both frozen and paraffin-embedded specimens. The present immunohistochemical study using a mouse monoclonal antibody describes the detection of Leu 7 antigen in a variety of neuroendocrine tumors which have been embedded in paraffin for routine histopathological diagnosis. Forty-nine (71%) of 69 such tumors showed varying degrees of Leu 7 immunoreactivity. At least in the tumors of the lung and pancreas, the number of Leu 7-positive cells was intimately related to the aggressiveness of the tumors: the more malignant the tumors were, the less the number of Leu 7-positive cells was. Leu 7 antigen was also demonstrated in normal fundic gland cells, thymic epithelial cells and colloid materials in normal thyroid follicles.
It can be concluded that Leu 7 antigen is a useful histochemical marker for normal and neoplastic neuroendocrine cells in the field of the endocrine pathology, and that investigations on the distribution of NK cells in tissue sections with anti-Leu 7 monoclonal antibody should be carried out with careful attention to these cross-reactions to non-lymphoid cell components.View full abstractDownload PDF (7963K) -
KOUTA YAMAMOTO, HIROSHI MAYAHARA, KAZUO OGAWA1984 Volume 17 Issue 1 Pages 23-35
Published: 1984
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSTo determine the localization of Na-K-ATPase activity in the rat hepatocyte, ouabain-sensitive, K-dependent p-nitrophenylphosphatase activity was studied cytochemically with a newly developed one-step lead citrate method devised by Mayahara et al. (1980). The liver was fixed with a mixture of 0.125% glutaraldehyde and 2% paraformaldehyde buffered with 30mM NaOH-PIPES instead of conventionally used 0.1M sodium cacodylate. Reaction products were localized predominantly on the sinusoidal and lateral membranes of the rat hepatocyte, which were remarkably abolished by addition of 10mM ouabain or by the deletion of K. They were localized on the cytoplasmic side of the membranes. Occasionally, reaction products were seen on the bile canalicular membrane, which were insensitive to ouabain and independent of K. These findings indicate that Na-K-ATPase is localized to the sinusoidal and lateral (basolateral) membranes of rat hepatocytes.View full abstractDownload PDF (16095K) -
KAZUYOSHI MIYATA, MASUMITSU TAKASUGI, MASA-OKI YAMADA1984 Volume 17 Issue 1 Pages 37-49
Published: 1984
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSWhen chlorotetracycline (CTC) was introduced into the DNA-ethidium bromide (EB) complex, the CTC fluorescence was interpolated into the EB fluorescence of DNA. The fluorescence parameters of EB and CTC could be measured biparametrically in relation to DNA structures. The EB fluorescence enhanced by intercalation into DNA was decreased by addition of CTC in vitro. Similar decrease of fluorescence occurred in cell nuclei isolated from rat liver cells and their chromatin.
On CsCl density gradient centrifugation, the peak position of the CTC-treated DNA was at a lower density than that of untreated DNA. On the sucrose density gradient centrifugation, CTC-treated chromatin was also at a lower density than untreated chromatin. When rats were treated with CTC in vivo, the fluorescence response of their liver DNA appeared to be similar to that in vitro.
Thus, biparametric fluoro-assay could be used for following structural change of DNA caused by CTC in vitro or in vivo.View full abstractDownload PDF (2309K) -
CARLO PELLICCIARI, CARLO A. REDI, SILVIA GARAGNA, MASARU FUKUDA, MARIA ...1984 Volume 17 Issue 1 Pages 51-58
Published: 1984
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe chromatin characteristics of spermatozoa taken from different portions of the female genital tract were analyzed by quantitative cytochemical reactions specific for DNA and thiol-rich protamines, in order to investigate if chromatin undergoes any structural modifications upon leaving the male genital tract.
When compared with the results obtained for vas deferens spermatozoa, all the data recorded after reactions specific for DNA (Feulgen reaction and staining with Propidium Iodide and Gallocyanin Chrome-Alum) and protamine thiol groups (DACM staining) suggest that chromatin remains highly stabilized during the passage along the female genital tract, and no sign of precocious decondensation may be detected before fertilization.View full abstractDownload PDF (840K) -
SHIGERU KATSURA, HIROSHI ISHIZUKA, HAYASHI MATSUMOTO1984 Volume 17 Issue 1 Pages 59-68
Published: 1984
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe histochemical organization observed in cross sections of rat lateral pterygoid muscle was investigated by staining for succinic dehydrogenase. Most primary fasciculi were composed of three muscle fiber types: Type A (white), Type B (intermediate) and Type C (red) according to the classification of Ogata (34) and Stein and Padykula (41). We found two distinct regions, one region with high average percentage distributions of number of Type B plus Type C fibers (Pbc) and type C fibers (Pc) values and the other region with low Pbc and Pc values in cross sections and significant differences in these values in different portions (origin, belly and insertion). Moreover, marked differences were found in the percentage distribution of Pbc and Pc in different primary fasciculi in each region of the lateral pterygoid muscle. In old rats the three types of muscle fibers were easier to distinguish and differences in the average Pbc and Pc values in different regions of the muscle were greater. Moreover, primary fasciculi with high Pbc and Pc values, or those with low Pbc and Pc values were more numerous than in young adult rats.View full abstractDownload PDF (3243K) -
TSUYOSHI SOJI1984 Volume 17 Issue 1 Pages 69-76
Published: 1984
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSHistochemical examinations of the alkaline phosphatase (ALPase) and the adenosine triphosphatase (ATPase) activities were performed on the anterior pituitary cells of male rats. ALPase was proved to be a powerful tool in visualizing the follicles. Electron microscopical observation showed that ALPase was positive on the microvilli of follicular cell surface. On the other hand, ATPase was found on the lateral plasma membranes of the follicular cells. ALPase and ATPase were found on different sites of the folliculo-stellate cell. The finding indicates a differentiation of the follicular cell plasma membrane.View full abstractDownload PDF (12450K) -
JUN MIYAUCHI1984 Volume 17 Issue 1 Pages 77-89
Published: 1984
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSImmunohistochemical localization of lactoferrin (LF) in human organs was studied light microscopically by means of PAP (peroxidase-antiperoxidase) method. Focusing on the submandibular gland, the localization of LF was compared with that of lysozyme (LZ) using double staining method and serial mirror-image sections, and subcellular localization of LF was studied with preembedding direct immunoperoxidase method. LF was distributed in the following sites: the salivary gland, the esophageal gland, the cardiac and pyloric glands, chief cells in the gastric gland proper, the gallbladder, the nasal and paranasal glands, the bronchial gland, neutrophilic granulocytes, thymic epithelium (Hassal's corpuscle), distal tubules and collecting tubules of the kidney, the seminal vesicles, the prostatic gland, the uterine cervical gland, intermediate lobe of the pituitary gland, the mammary gland in lactating phase, and the lacrimal gland. In the submandibular gland, LZ-positive serous cells were always less numerous than LF-positive cells, and intermingled among LF-positive acini. LZ-containing cells also occasionally contained LF. Electron microscopically, LF was observed in the serous granules of acinar cells in the submandibular gland. Central core of the granules stained intensely whereas peripheral rim stained weakly. LF was not observed in other cell organelles.View full abstractDownload PDF (16270K)
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