ACTA HISTOCHEMICA ET CYTOCHEMICA

ELECTRON-CYTOCHEMICAL STUDIES ON THE DIFFERENTIATION OF MOUSE LUNG ALVEOLAR EPITHELIAL CELLS WITH SPECIAL REFERENCE TO CHANGES IN MITOCHONDRIA

KEI-ICHI HIRAI, TADASHI UYEDA, KAZUO OGAWA

1984 Volume 17 Issue 3 Pages 197-211
Published: 1984
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.17.197

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Changes in mitochondrial structures and cytochrome oxidase activities were electron-cytochemically observed in lungs of butylated hydroxytoluene (BHT)-administered mice. The cytochrome oxidase activity was demonstrated by the diaminobenzidine method, and changes in the mitochondrial sizes during the differentiation were measured by morphometric analysis.
BHT selectively damaged Type I cells, and then Type II cells differentiated to Type I cells. When Type II cells differentiated to Type I cells, the mitochondria were greatly reduced in size and number. The mitochondria in intermediate cells became slender and divided into smaller mitochondria. It is possible that the amount of cytochrome oxidase per mitochondrion decreased. Thus, these cytochemical approaches evidenced functional as well as ultrastructural changes that occurred during the alveolar differentiation.

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THE DISTRIBUTION OF RADIOACTIVE CARBON FROM [U-¹⁴C] GLUCOSAMINE IN MICE

HIDEYUKI GOTO, MASAHITO WATANABE, RYOICHI SHIMONO, TAKASHI KIHARA

1984 Volume 17 Issue 3 Pages 213-222
Published: 1984
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.17.213

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Tissue and organ distribution of radioactive carbon from ¹⁴C-labeled glucosamine in the mouse was studied by whole-body autoradiography and biochemical analysis. The mice injected intraperitoneally with D-[U-¹⁴C]-glucosamine were sacrificed at various intervals. Examination of autoradiographs disclosed that the injected ¹⁴C-glucosamine was almost completely absorbed from the abdominal cavity 30min after injection. At 5min after injection, the highest radioactivity was observed in the liver, but the activity in the blood and brain was low. The radioactivity in these organs was made negligible by perchloric acid treatment of sections. At 30min after injection, high radioactivity was detected in the liver, small intestine, preputial gland and urine. At 3hr after injection, the liver, blood and cortex of the kidney had high radioactivity.
The comparative values among radioactivity in various organs estimated by a liquid scintillation counter were consistent with those obtained from whole-body autoradiographs. At 30min after injection, an increase of radioactivity in the acid-insoluble fractions was observed in all organs examined. In animals at 5 and 30min after injection, paper chromatography of the acidsoluble fractions disclosed radioactive spots for glucosamine and N-acetyl-glucosamine in the blood, N-acetylglucosamine and glucose in the kidney and glucosamine, glucuronic acid and glucose in the liver. By gel electrophoresis, a radioactive protein with molecular weight of 90, 000 was detected in the acid-insoluble fraction of kidney at 30min after injection.

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IMMUNOHISTOCHEMICAL LOCALIZATION OF SECRETORY COMPONENT AND IGA IN THE HUMAN ENDOMETRIUM IN RELATION TO MENSTRUAL CYCLE

MITSUAKI SUZUKI, MASATOSHI OGAWA, TARO TAMADA, HIROSHI NAGURA, KEIICHI ...

1984 Volume 17 Issue 3 Pages 223-229
Published: 1984
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.17.223

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To define the changes in the distribution of secretory component (SC) and IgA in the endometrium associated with the menstrual cycle, and endometrial tissues of 25 cases showing a normal menstrual cycle were immunohistochemically investigated by the peroxidase-labeled antibody method. SC was found in the epithelial cells of endometrial glands and the secretion within their lumen. In the functional layer, the staining intensity was very weak during the period from the proliferative to the early secretory phases, and much increased in the mid and late secretory phases. In the basal layer, however, SC was rather strong and did not change throughout the menstrual cycle. The epithelial staining of IgA resembled that of SC, the staining intensity, however, was much weaker than that of SC. In addition, IgA also was stained in the endometrial stroma as well as vascular lumens and plasma cells.
Based on the above results, it is suggested that SC-mediated transcellular transport of IgA occurs across the epithelial cells of endometrial glands, and that it is regulated by the menstrual cycle.

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BINDING OF PEPSIN-TREATED HUMAN IMMUNOGLOBULIN TO THE BACTERIAL CELL SURFACE: AN IMMUNOCYTOCHEMICAL STUDY

JUNKO UCHIYAMA, AKIRA KOSHIRO, SUSUMU TOMONAGA

1984 Volume 17 Issue 3 Pages 231-239
Published: 1984
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.17.231

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A fibrous substance was consistently observed on the cell surface of bacteria, K. pneumoniae and S. aureus, after incubation with a pepsin-treated human immunoglobulin. Chemical component of the fibrous substance was studied by an immunocytochemical technique. The major component of the fibrous substance was demonstrated to be human immunoglobulin.

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HISTOLOGIC STAINING METHODS FOR HEPATITIS B SURFACE ANTIGEN (HBS AG) AND INTERPRETATION OF DYE REACTIONS IN VITRO

MASACHIKA SENBA

1984 Volume 17 Issue 3 Pages 241-249
Published: 1984
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.17.241

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Comparison of five HBs Ag staining methods were carried out in 303 autopsy cases and 398 biopsy cases of various liver diseases, including acute hepatitis, chronic active hepatitis, chronic inactive hepatitis, hepatocellular carcinoma without cirrhosis, hepatocellular carcinoma with cirrhosis, and cirrhosis. The author tried various HBs Ag staining dyes which react with some functional groups and found that all HBs Ag staining dyes react with hydrogen sulfide, disulfide and sulfonic acid residue groups which are formed by the oxidization of disulfide and hydrogen sulfide groups of proteins. Three mechanisms of nonspecific reaction in HBs Ag staining methods were considered.

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IMMUNOCYTOCHEMICAL LOCALIZATION OF S-100 PROTEIN AND CALMODULIN IN C6 GLIOMA CELLS

MAMORU SANO, KANEFUSA KATO, AKIKO SETO-OHSHIMA, AKIRA MIZUTANI

1984 Volume 17 Issue 3 Pages 251-258
Published: 1984
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.17.251

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The localization of S-100, calmodulin and tubulin in the cultured C6 glioma cells was observed with the indirect immunoperoxidase method. The direct immunoperoxidase method for S-100 was also employed. The reaction products for S-100, calmodulin and tubulin were all found over filamentous structures in the interphase cells. Calmodulin and tubulin seemed to be localized in the cytoplasm, while S-100 was exhibited as intense granular reaction product in the nuclei.
In the mitotic cells, these proteins were shown to be present with characteristic localization in the mitotic apparatus. Calmodulin-specific staining was highly intense at the spindle poles.
In contrast, S-100 was shown to be concentrated on the chromosomes in all phases of mitotic cells. The direct method showed more accurate and defined localization of S-100 than the indirect method. This is the first demonstration of the possible interrelationship between S-100 protein and chromosome, although the biological significance of the fact is not known at present.

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DISTRIBUTION OF N-ACETYL [4, 5, 6, 7, 8, 9-¹⁴C] NEURAMINIC ACID IN MICE STUDIED BY WHOLE-BODY AUTORADIOGRAPHY

RYOICHI SHIMONO, MASAHITO WATANABE, NARUTO KUNO, TAKASHI KIHARA

1984 Volume 17 Issue 3 Pages 259-268
Published: 1984
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.17.259

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The distribution of N-acetyl [4, 5, 6, 7, 8, 9-¹⁴C] neuraminic acid ([¹⁴C]-NeuNAc) was investigated by whole-body autoradiography. At 5min after intravenous injection of [¹⁴C] NeuNAc, high radioactivity was found in certain tissues and organs such as the blood, kidney, urine, bulbourethral gland, skin, and connective tissues. However, the radioactivities of all tissues and organs showed an abrupt decrease during the first 30min, and after 3hr, only the urine, kidney and distal portion of the small intestine had any traces of radioactivity. These autoradiography provide the first evidence that exogenously administered [¹⁴C] NeuNAc is taken up highly by the bulbourethral gland and that there are certain differences in intensity of radioactivity within or among the organs.

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MG⁺⁺-DEPENDENT ADENOSINE TRIPHOSPHATASE ACTIVITY IN PREIMPLANTATION MOUSE EMBRYOS

TOMOICHI ISHIKAWA, HARUMICHI SEGUCHI

1984 Volume 17 Issue 3 Pages 269-278
Published: 1984
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.17.269

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Mg⁺⁺-dependent adenosine triphosphatase (Mg-ATPase) activity was cytochemically investigated in preimplantation mouse embryos. This enzyme activity was demonstrated on the cell surface of unfertilized and fertilized eggs, blastomeres in cleavage-stage embryos and blastocysts. In blastocysts, however, only trophectodermal cells were positive for this enzyme reaction. Among these cells, mural trophectodermal cells had more intense activity of Mg-ATPase than polar trophectodermal cells. Inner cell masses of blastocysts showed absolutely no Mg-ATPase activity.

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IDENTIFICATION OF NEONATAL BRAIN MACROPHAGES BY LECTIN-HISTOCHEMISTRY

TOSHIHIKO MIYAKE

1984 Volume 17 Issue 3 Pages 279-282
Published: 1984
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.17.279

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RAPID PURIFICATION OF MONOCLONAL ANTIBODY IN ASCITES BY HIGH PERFORMANCE ION EXCHANGE COLUMN CHROMATOGRAPHY FOR DIMINISHING NON-SPECIFIC STAINING

KACHIO TASAKA, MAKIO KOBAYASHI, TOSHIZO TANAKA, CHIYOKO INAGAKI

1984 Volume 17 Issue 3 Pages 283-286
Published: 1984
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.17.283

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IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF CALMODULIN WITH PROTEIN A-GOLD TECHNIQUE

NOBORU YAMAMOTO

1984 Volume 17 Issue 3 Pages 287-291
Published: 1984
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.17.287

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