巻号一覧
17 巻 (1984)
- 6 号 p. 615-
- 5 号 p. 461-
- 4 号 p. 297-
- 3 号 p. 197-
- 2 号 p. 93-
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17 巻, 4 号
選択された号の論文の15件中1～15を表示しています
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LIGHT AND ELECTRON MICROSCOPIC AUTORADIOGRAPHIC STUDIES USING 3H-QUINUCLIDINYL BENZILATEMASAHIKO NAKAMURA, MASAYA ODA, YOSHIKAZU YONEI, NOBUHIRO TSUKADA, NORI ...1984 年 17 巻 4 号 p. 297-309
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーFor autoradiographic demonstration of the muscarinic acetylcholine receptor (m-AChR) in the gastric mucosa, 3H-quinuclidinyl benzilate (3H-QNB), a potent muscarinic antagonist, was administered to rats at a constant rate of 1.4ml per hr via an aortic catheter. Fixation of the tissue was made by a freeze-drying procedure, followed by the wet-sectioning of the Epon blocks with ethylene glycol and the dry-mounting of the emulsion to obliterate loss of the water soluble radiolabeled antagonist. To examine the specificity of the autoradiographic reaction, the atropine-treated and the vagotomized rats were also treated in the same manner as the control rats.
As a result, it has been confirmed that the parietal cell among the gastric epithelial cells is a major site of labeling of 3H-QNB, whereas few grains are located over the surface epithelium. Some of the grains were also closely associated with the true capillary endothelium in the gastric mucosa. Electron microscopic autoradiography revealed that most of the grains were in close proximity to either the plasma membrane of the parietal cell or the basal plasma membrane of the true capillary endothelium. Preadministration of atropine resulted in a decrease of the labeling of 3H-QNB, whereas the vagotomy caused an increase in the labeling.
The above-findings strongly support our hypothesis previously proposed on the basis of the localization of the acetylcholinesterase, that the parasympathetic nerve would have some direct effects on the true capillary as well as on the parietal cell.
In addition, the m-AChR was found to be dispersed both on the true capillary endothelium and on the parietal cell plasma membrane by electron microscopic autoradiography, compared with the nicotinic AChR in the neuromuscular junction.抄録全体を表示PDF形式でダウンロード (18326K) -
HIROSHI MATSUDA, HISAO FUJITA, KAZUNORI ISHIMURA, YOSHIO OSAWA1984 年 17 巻 4 号 p. 311-322
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーIn order to clarify the in vivo localization of aromatase in the ovarian tissues of rat, mouse, guinea pig, golden hamster, cow and human, immunocytochemical investigations using the anti-human placental aromatase II cytochrome P-450 antibody were carried out by the PAP or indirect fluorescent antibody method.
Immunoreactive aromatase was detected on the theca interna, as well as the interstitial gland cells of both immature and mature rodent ovaries and the corpus luteum cell of mature rodent ovaries. The immunoreactivity was also demonstrated on the theca interna cell and corpus luteum cell of cow and human ovaries. Only in large preovulatory follicles of the mature rodent ovary did the granulosa cell react with the antibody of this enzyme, while granulosa cells in all the other follicles were consistently negative in this immunoreaction.
These results might imply that the theca interna cell and interstitial gland cell presumably have a fundamental ability of the aromatization from androgens to estrogens in the mature and immature ovaries.抄録全体を表示PDF形式でダウンロード (14268K) -
FUMIAKI NISHIYAMA, KUNIAKI TAKATA, HIROSHI HIRANO1984 年 17 巻 4 号 p. 323-330
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーTo study the flow of membrane constituents during the course of endocytosis, cytochemically-demonstrable Mg2+-adenosine triphosphatase (Mg2+-ATPase) activity was chosen as a marker of the cell membrane and was detected during the course of the endocytosis of cationized ferritin (CF) by the double labeling technique. Mouse peritoneal macrophages were incubated with CF at 37°C for 30-60min, fixed, and then processed for the demonstration of Mg2+-ATPase activity. Mg2+-ATPase activity was found in CF-labeled vesicles and vacuoles. Mg2+-ATPase activity, however, was not detected in multivesicular bodies, in lysosomes, or in the Golgi apparatus, although endocytosed CF was found in these organelles. These results suggest that Mg2+-ATPase was co-internalized with CF and that its activity was lost during the translocation to lysosomes and the Golgi apparatus, presumably owing to inactivation and membrane recycling.抄録全体を表示PDF形式でダウンロード (8733K) -
TADANOSU BAN, KAZUNORI ISHIMURA, HISAO FUJITA, KENJI SOBUE, SHIRO KAKI ...1984 年 17 巻 4 号 p. 331-338
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーThe topographical distribution of caldesmon (a calmodulin-binding, F-actin-interacting protein) and of actin was investigated in various organs of rats such as esophagus, intestine, submandibular gland, pancreas, adrenal gland, ovary, uterus, oviduct, kidney, skeletal muscle, heart, and aorta by means of light microscopic immunohistochemistry.
Positive immunostaining for caldesmon was observed in smooth muscle cells of all the organs examined, and myoepithelial cells of the submandibular gland. In addition, caldesmon was also present in the apical cytoplasm of the epithelial cells of the intestine, uterus, oviduct, submandibular gland, and pancreas and renal tubules, and in the peripheral cytoplasm of adrenal medullary cells.
The distribution of actin in these tissues shows almost the same pattern as that of caldesmon, though caldesmon was not detected in skeletal and cardiac muscles.
It is concluded that caldesmon is generally present not only in smooth muscle cells but also in non-muscle cells of various organs. This result suggests that the calmodulin-binding protein, caldesmon, may play an important role in the control of actin-myosin interaction of these cells.抄録全体を表示PDF形式でダウンロード (9246K) -
THE DISTRIBUTION AND POSSIBLE SIGNIFICANCE OF NATURAL KILLER CELLS (HNK-1+ CELL) IN THE HUMAN SPLEENHISAKO TANAKA, SATOSHI TAKASAKI, TETSUYA MUROYA, TERUO SUZUKI, EISEI I ...1984 年 17 巻 4 号 p. 339-358
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーThe distribution of natural killer cells in human spleen was studied immunohistochemically and immuno-electron microscopically using a monoclonal antibody for human natural killer cell (HNK-1). The reactivity of this antibody to the formalin fixed and paraffin embedded specimens was confirmed and technical improvement was attempted. HNK-1+ cells were divided into two groups according to their distribution and cytological characteristics. One group consisted of cells distributed around the white pulp and in the red pulp and the other group localized in the germinal center (GC). The former morphologically resembled large granular lymphocyte (LGL) of peripheral blood and was thought to be the LGL of the blood circulating in the spleen. Although the latter consisted of lymphocytes having electron dense granules as LGL, their size and the ratio of nucleus to cytoplasm were greater than those of the former and their ultrastructural appearance indicated promoted secretory activity. The number of HNK-1+ cells in GC was intimately related to the development of GC. Furthermore, their population correlated with the number of mitoses of centroblasts, tingible body macrophages or peanut lectin-bound immature B lymphocytes, respectively. Based on these facts, a possibility was suggested that HNK-1+ cells in GC might be in an active phase and they might be related to the physiological regulation of differentiation and expansion of B cell. Phenotypic expression of HNK-1+ cells was examined by double immunoenzymatic labelling, but no differences were found between the HNK-1+ cells in GC and in the red pulp.抄録全体を表示PDF形式でダウンロード (28841K) -
ISAMU TAKAGI, KEIKI YAMADA, NOBUYUKI KARASAWA, IKUKO NAGATSU1984 年 17 巻 4 号 p. 359-370
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーSubcellular localization of tyrosine hydroxylase (TH) in the ultimo-branchial body of the grass parakeet was demonstrated at the electron microscopic level, using the peroxidase antiperoxidase (PAP) technique. Comparison between TH-positive cells and calcitonin (Cal)-immunoreactive cells revealed that the latter were more numerous than the former. The average diameter of the TH-positive granules was 230-290nm and very close to the size of Cal-positive granules (200-320nm). By conventional electron microscopy, four types of granular cells were observed in the ultimobranchial body of the grass parakeet: A-type cells containing granules 200-320nm in diameter, B-type characterized by granules of 150-270nm with or without vacuoles, C-type with granules of 130-210nm, and D-type with granules of 80-170nm. TH and/or Cal-immunoreactive granules corresponded to A- or B-type cells based on the size of the granules.抄録全体を表示PDF形式でダウンロード (13980K) -
INMACULADA SÁNCHEZ-AGUAYO, JOSEFINA HIDALGO, FELIPE CORTES, JOS ...1984 年 17 巻 4 号 p. 371-377
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーUltracytochemical analysis of acid phosphatase in the nephrocytes of Helix aspersa renal sac was carried out using beta-glycerophosphate as the substrate of reaction. The reaction product was localized on dictyosomes, lysosomes, apical and lateral plasma membranes and, occasionally, in dense digestive bodies. In the latter, the lack of reaction or the weakness of it is discussed. Reaction product was affected by sodium fluoride, except in apical and the most lateral membranes. This fact could be interpreted as the result of phosphatase activity at these sites being different from that of lysosomal acid phosphatase.抄録全体を表示PDF形式でダウンロード (7425K) -
F.J. MORENO, J. PIÑERO, R. COVEÑAS, J.L. LÓPEZ-CA ...1984 年 17 巻 4 号 p. 379-386
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーMorphological modifications and cytochemical changes in the acid phosphatase on oxintic cells of the gastric mucosa of rats deprived of food for varying lengths of time were studied. With long fasting periods a lysosomal qualitative increase and mitochondrial morphological alteration are caused. This last fact would make an ATP decrease and an alteration in the ATPase actuation, so preventing the interchange of H+ for K+ and thus blocking intra-vesicular HCl formation.抄録全体を表示PDF形式でダウンロード (14903K) -
YUKARI KAWAKAMI-KONDO, MASAMI YOSHIDA, NOBUYUKI KARASAWA, KEIKI YAMADA ...1984 年 17 巻 4 号 p. 387-397
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーBy the immunofluorescence method, development of the cells containing catecholamine-synthesizing enzymes [tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH) and phenylethanolamine-N-methyltransferase (PNMT)], serotonin (5-HT), calcitonin (Cal), somatostatin (Som), β-endorphin (End) and adrenocorticotropin (ACTH) was examined in the pituitary and tubero-infundibulum of Rana catesbeiana tadpoles, and the localization was compared with that of adult bullfrogs.
A large number of End- and ACTH-like immunoreactive cells were observed in the rostral part of the pars distalis of the sadpole pituitary before metamorphosis (stage 31 by Gosner). At this time, 5-HT-positive cells were also observed throughout the pars distalis, though far less than those of the adult frog. TH-positive cells first appeared at Gosner's stage 40 together with the appearance of hindlimbs, but these cells were DBH-negative. The cells reacting with antiserum against DBH appeared after the resorption of the tail.
TH-positive, DBH-negative dopaminergic fibers were observed around the capillary sinusoid of the pars distalis at Gosner's stage 31, but at stage 40 these fibers disappeared.
TH-, 5-HT-, Cal-, Som- and End-immunoreactive neurons in the hypothalamus, and their fibers which may be derived from this brain area, in the neural lobe and median eminence, were already observed in the tadpole before metamorphosis (stage 31), as those in the adult frog.抄録全体を表示PDF形式でダウンロード (12216K) -
KYUNG-HWA. HYUN, SHINICHIRO SUMITOMO, SHIN FUKUI, MASAHIKO MORI1984 年 17 巻 4 号 p. 399-413
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーSugar residues in the epithelial cells of verruca vulgaris and seborrheic keratosis were demonstrated by the horseradish peroxidase-conjugated lectin methods. The lectins, Con A, RCA-1, UEA-1, DBA, PNA, SBA, and WGA were used to bind specifically to the sugars, Man, Glc, Gal, Fuc, GalNAc, GlcNAc and NANA. The cytochemical distribution of lectin bindings were limited to the cell surface, intercellular materials and basement membrane in the epithelial structures of the lesions. The lectin bindings in the basal layer cells were negative or weak, but increased gradually in upper spinous cells from moderate to strong levels. Vacuolated cells in verruca vulgaris showed positive lectin stainings. The intercellular materials of parakeratinized cells bound lectins differently, but orthokeratinized cells failed to bind lectins. The regional distribution of lectin bindings in stratified squamous epithelia may indicate an expression of keratinocytic stratification as well as in keratinized benign lesions.抄録全体を表示PDF形式でダウンロード (21755K) -
D. N. VISHNOI, S. NARAIN1984 年 17 巻 4 号 p. 415-420
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーEsterase activity has been studied for the first time during different phases of cellular activity i.e. growth, prefunctional, functional and post functional (degenerating) ones in the spinning gland cells of the Indian moth D. obliqua. Esterase activity is in keeping with the needs of energy of the growing spinning gland cells which is associated with increased metabolism of the cell rather than the secretion of silk. The enzyme also seems to play a significant role in the termination of larval period.抄録全体を表示PDF形式でダウンロード (4032K) -
TATSUYA TAKANO, KIMIKO AMANUMA-MUTO, TSUNEO IMANAKA, SHOJI OHKUMA1984 年 17 巻 4 号 p. 421-426
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーCulture of foam cells isolated from rabbit atheromatous aorta by treatments with collagenase, elastase and hyaluronidase was achieved. The cells adhered to the glass culture dish and spread out. Under a polarized microscope, anisotropic cross images were clearly seen in the cells, suggesting that the anisotropic lipid inclusions (Lang & Insull, 1970(4) and Takano et al., 1982(9)) were not artifacts but existed in vivo. The characteristics of the cultured cells were examined by cinemicrophotography. Some cells were in contact with each other, whereas others were not. Endocytic vesicles were frequently observed in periplasmic areas, indicating that the cells possessed high endocytic activity.抄録全体を表示PDF形式でダウンロード (6379K) -
SHIGERU ENTANI, ETSUO CHIHARA, SATOKI UENO, HIROYUKI OKAMOTO1984 年 17 巻 4 号 p. 427-433
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーThe localization and concentration of protein-bound sulfhydryl groups and disulfide linkages in mouse ocular tissues were examined with a new fluorescent thiol reagent, N-(7-dimethylamino-4-methylcoumarinyl)-maleimide (DACM). Corneal epithelium and endothelium, conjunctival epithelium, lens cortex, retinal plexiform layers and inner segment and pigment epithelium were rich in SH groups. Descemet's membrane and lens capsule contained many S-S linkages. Corneal stroma, sclera and retinal outer segment contained a moderate number of S-S linkages. There was a unique distribution of SH groups and S-S linkages in the eye; that is, tissues abundant in SH groups contained few S-S linkages, and tissues abundant in S-S linkages contained few SH groups. The role of SH groups and S-S linkages in the eye was discussed.抄録全体を表示PDF形式でダウンロード (5972K) -
TORU NODA, KAZUO OGAWA1984 年 17 巻 4 号 p. 435-451
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーThe three-dimensional structure of the Golgi apparatus (GA) in mouse pancreatic exocrine cells was studied under an ordinary transmission electron microscope and also a high voltage electron microscope. From observations of thick sections of ZIO (Zinc-Iodide-Osmium solution)-reacted pancreatic tissues, GA was found to be a complex three-dimensional structure with its basic configuration summarized as follows: 1. Golgi cisterna is a long elongated ribbon-like structure and Golgi stack is composed of several ribbon-like cisternae organized from cis to trans. 2. The ribbon-like Golgi stack has wide expanded areas in some portions where it branches into 2 or 3 ribbon-like stacks. 3. The Golgi stack often shows a huge circular arrangement, about 10μm in diameter, maintaining the polarity from cis to trans. 4. In some portions the direction of polarity of the stack changes: the Golgi stack in some parts rotates in the long expanding direction (X axis) maintaining each cisternal continuity. 5. According to the observations of 1μm thick serial sections, the continuity and polarity of the Golgi stack are maintained three-dimensionally (X, Y and Z axes) and GA, as a whole, was found to be one huge continuous reticular organelle in the cell.抄録全体を表示PDF形式でダウンロード (28298K) -
KAZUSHI FUJIMOTO, KAZU SHIGE OGAWA, KAZUO OGAWA1984 年 17 巻 4 号 p. 453-456
発行日: 1984年
公開日: 2009/10/28
ジャーナル フリーPDF形式でダウンロード (3078K)
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