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Volume 18 (1985)
- Issue 6 Pages 565-
- Issue 5 Pages 467-
- Issue 4 Pages 365-
- Issue 3 Pages 275-
- Issue 2 Pages 139-
- Issue 1 Pages 1-
Volume 18, Issue 5
Displaying 1-9 of 9 articles from this issue
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KAZUSHI FUJIMOTO, KATSUHIKO NARIMOTO, KAZU SHIGE OGAWA, SETSUKO KONDO, ...1985 Volume 18 Issue 5 Pages 467-482
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe gap junction contains an intercellular channel allowing the passage of ions, small molecules and cyclic nucleotides etc., and is involved in the intercellular communication between adjacent cells. The focus of this review is to describe the recent progress in the following cytochemical aspects of gap junction, and some implications for structure and function of gap junction are discussed.
1. Enzyme cytochemistry: Adenylate cyclase, guanylate cyclase and Ca2+-ATPase activities are localized on the cytoplasmic side and/or the inner leaflet of gap junctional membranes.
2. Cytochemical demonstration of hydrophilic channel: Hydrophilic channel can be visualized under an electron microscope using alkali-metal ions.
3. Cytochemical demonstration of charge on gap junction: Cytoplasmic side of gap junction shows the high density distribution of anionic sites.View full abstractDownload PDF (21387K) -
MATTHEW GRAHAM WHITE, DAVID H. SNOW1985 Volume 18 Issue 5 Pages 483-493
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSNumerous subjective methods have been used for the classification of mammalian skeletal muscle fibre types. The value of SDH and myosin ATPase (mATPase) after acid preincubation for subdividing fast (type II) muscle fibre types in equine skeletal muscle, is assessed. Subjective visual assessment of the reaction intensity following SDH and mATPase after acid preincu bation is compared with microdensitometric measurements of the same muscle fibres. The relationship of SDH reaction product to mATPase reaction intensity is examined.
In the samples studied, visual assessment indicated that there appears to be two distinct reaction intensities for SDH. However, microdensitometry shows that the distribution of absorbances is over a continuous range of values with distinct peaks. The mATPase reaction after acid preincubation allowed visual discrimination of 4 reaction intensities, types I, IIab and IIa, in descending order of reaction intensity. However, microdensitometry showed a continuous range within the type II fibres with two or three overlapping peaks.
Plotting SDH absorbance versus mATPase absorbance showed that in some cases 4 clusters of fibres were present and that they compared well with the subjective classification by mATPase after acid preincubation. However, one case displayed only three clusters. The IIa and IIab groups overlapped.
These data are consistent with the hypothesis that IIab fibres are transitional between IIa and IIb.View full abstractDownload PDF (3409K) -
SOTOKICHI MORII, NOBUTOSHI KOTANI, NOBUAKI SHIKATA1985 Volume 18 Issue 5 Pages 495-503
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSTechnical improvements for the ultracytochemical demonstration of lining layer of the lung alveoli in adult rats are as follows: (1) Vascular perfusion of a twice diluted Karnovsky's fixative was applied to the lung tissues after air instillation into the airways, and minute tissue blocks were prepared by quick freezing and trimming of the prefixed lung. (2) Enzymic digestive method using a purified phospholipase C was performed in the floating prefixed minute tissues. After postfixation in cold osmium buffer, acetone was used for dehydration of the digested blocks to preserve phospholipids. (3) Ultracytochemical procedure using ruthenium red for mucopolysaccharides was carried out in the similarly treated minute lung blocks, and wire-meshed small cages were utilized as carriers to prevent floating throughout these procedures. Reaction products after phospholipase-digestive method, probably indicating the site of phospholipids, are clearly demonstrated on the surface film of the alveolar lining layers. Ruthenium red is abundantly visible in whole zones of acellular lining layer of the lung alveoli.View full abstractDownload PDF (16285K) -
MATSUJI HOSAKA, YUKIHIRO TATEMOTO, TAKAHIRO YAMAGAMI, NOBUYUKI HIKOSAK ...1985 Volume 18 Issue 5 Pages 505-514
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSImmunohistochemical demonstration of intermediate filament proteins-keratin, vimentin and desmin, and microfilaments-actin, filamin and myosin in the parotid and submandibular glands of humans are described. All ductal segments of intercalated, striated excretory ducts indicated varying staining to filament proteins: keratin was strong, vimentin, actin, filamin and myosin moderate and desmin slight in staining levels. Stainability of the intercalated duct was less than other ducts to desmin, actin, filamin, and myosin. Proteins in acinar cells showed no reaction or a weak staining reaction. Actin staining was characteristically strongly positive in myoepithelial cells. Histochemical reactions of desmin and actin were positive in smooth muscle in blood vessels, and that of vimentin in connective tissue fibers (Table 1).View full abstractDownload PDF (13732K) -
TOSHIRO ONO, TADASHI TSUJII, SATIMARU SENO1985 Volume 18 Issue 5 Pages 515-524
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSObservations were made on rat peritoneal macrophages exposed to cationic ferric hydroxide colloid (ferric hydroxide-cacodylate complex, Fe-Cac) in vitro in order to study the process of endocytosis of the cationic particles and their fate after ingestion. Fe-Cac particles given to macrophages were adhered to the cell surface and taken up through endocytosis. The ingested particles were first found in the cytoplasmic tiny vesicles and canals adhering to their inner surfaces. These tiny vesicles seldom fused each other or with lysosomes at the early stage of incubation. By further incubation in the iron-free medium, however, macrophages developed the acid phosphatase positive large vacuoles containing a quantity of iron particles, showing the fusion of some Fe-Cac containing endocytic vacuoles with lysosomes. After 15hr of chase in iron-free medium ferritin molecules in paracrystalline arrangements appeared in a few siderosomes and also diffusely scattered ones in cytoplasmic matrix. Immunocytochemical observations of ferritin with antirat ferritin antibody and FITC conjugated rabbit IgG showed diffuse staining of cytoplasm after 10 to 15hr of chase in iron-free medium.View full abstractDownload PDF (15931K) -
KATSUHIRO NARIMOTO, HITOSHI OKAMURA, TAKAHIDE MORI, MASAHIRO SAKAI, L. ...1985 Volume 18 Issue 5 Pages 525-537
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe different cell layers in the follicle wall in rabbit ovaries were examined for acid phosphatase activity during the ovulatory process. The tissue was taken before the process began and at 5, 7, 9, 10 and 11hr after it was induced by human chorionic gonadotropin. The ovaries were perfused with a glutaraldehyde fixative, cut with a microslicer, incubated for 60min at 37°C in Gomori's acid phosphatase medium, and then routinely prepared for light and electron microscopy. Control tissue, which was incubated with the acid phosphatase inhibitor, sodium fluoride, did not reveal any enzyme activity. The results show acid phosphatase reaction product in all the major cell types in the follicle wall. The enzyme is located mainly in lysosomes and Golgi apparatus. It is not present in the electron-dense granules which are characteristic of the cytoplasm of surface epithelial cells. The acid phosphatase in the thecal layers of the follicle appears to be localized, not only in lysosomes and Golgi apparatus, but also in the multivesicular structures which are present in the thecal fibroblasts of this tissue. Collectively, the data indicate that acid phosphatase and other lysosomal enzymes may contribute, at least in part, to the hydrolytic degradation of follicular connective tissue during ovulation.View full abstractDownload PDF (21937K) -
KIHACHIRO HORIIKE, RYOHACHI ARAI, HIROMASA TOJO, TOSHIO YAMANO, MITSUH ...1985 Volume 18 Issue 5 Pages 539-550
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA histochemical method for the demonstration of D-amino acid oxidase activity in rat kidney, liver and brain is described. The method is a peroxidase-coupled procedure, which is based on the intensifying effect of nickel ions on 3, 3′-diaminobenzidine-based product formation with peroxidase. The substrates used were D-proline and D-alanine, of which the former showed more intense staining. The histochemical reaction was suppressed by benzoate, a competitive inhibitor. There was no staining on omission of a substrate or peroxidase from the incubation medium. The method was specific for D-amino acid oxidase. The oxidase in kidney and brain showed resistance to the fixatives used (e. g. 8% glutaraldehyde), while the enzyme in hepatocytes did not. The reaction product was black, electron dense, stable and strictly confined to cells containing the oxidase. The method was successfully applied to the histochemical identification of the type of cell containing the oxidase at both the levels of light and electron microscopy. The oxidase activity was shown to be present in hepatocytes in the liver, proximal tubule cells in the kidney, and only astrocytes, including Bergmann glial cells, in the brain. In the midbrain, the oxidase was almost exclusively restricted to the tegmentum.View full abstractDownload PDF (11435K) -
MAKIO KOBAYASHI, TATSUO SAWADA, NORITSUGU MUKAI1985 Volume 18 Issue 5 Pages 551-556
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSRetinoblastoma-like tumor cells (EXP-5 cell line) derived from a tumor in an inbred albino rat by human adenovirus 12 have been maintained in culture. We investigated immunocytochemical localization of neuron specific enolase (NSE) and S-100 protein in cultured tumor cells before and after successful differentiation into neuronal and glial-like cells. After the addition of cyclic AMP analog, a neurite-like process developed, and they tended to form a network after 24hr. Before and after successful differentiation, the cultured cells exhibited simultaneous expression of NSE and S-100 protein. Differentiated cells showed a tendency to express the marker that correlated with their morphology. These results supported the idea that adenovirus 12-induced retinoblastoma-like tumor was derived from bipotential precurcer cells.View full abstractDownload PDF (2436K) -
TERUO TANAKA, MAKOTO TANAKA, AKIRA SAKANO1985 Volume 18 Issue 5 Pages 557-561
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSDownload PDF (8806K)
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