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Volume 2 (1969)
- Issue 4 Pages 147-
- Issue 3 Pages 83-
- Issue 2 Pages 39-
- Issue 1 Pages 1-
Volume 2, Issue 2
Displaying 1-5 of 5 articles from this issue
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H. B. TEWARI, P. P. SOOD1969 Volume 2 Issue 2 Pages 39-53
Published: 1969
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA comparative study of distribution of alkaline and acid phosphatases, 5-nucleotidase and ATPase in various regions of peripheral and central nervous system of a large variety of animals i. e. in the nuclei of the neurons of olfactory lobes of rat, bat, guinea-pig, mouse, cat, rabbit, frog, chicken, turtle and uromastix, trigeminal ganglia of cat, dog and turtle, spinal ganglia of turtle, vagus ganglia of frog, primordial hippocampal region of turtle and frog, entopeduncular nucleus of frog and cerebellum of cat have presented specific patterns of distribution in the nucleolar and nuclear sites. In some cases the nuclei have revealed absence of activity for certain enzymes. The data obtained from the comparative study, encampassing such a large number of neurons, suggest that positive sites demonstrated by various methods for phosphatases i. e. by Takamatsu-Gomori technique for alkaline phosphatase, Gomori technique for acid phosphatase and Wachstein and Meisel methods for 5-nucleotidase and ATPase, do reveal real locales of enzymatic activity and are not artifactual in nature. The metabolic significance of the intranuclear distribution of phosphatases has been also discussed.View full abstractDownload PDF (20557K) -
TAKAO KAWASHIMA, FUMIO MURATA1969 Volume 2 Issue 2 Pages 54-60
Published: 1969
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSElectron microscopy for the cytochemistry of cholinesterase was performed in the human intercostal muscle by using an indoxyl acetate esterase method.
The enzyme activity was visible as a fine and nondroplet electron opaque endproduct in the axonal plasma membrane, the sarcolemmal plasma membrane of the muscle fiber, the junctional cleft, the endoneurium, and unknown membrane system between the muscle fibers.View full abstractDownload PDF (6432K) -
KATSUYOSHI MIZUNO, IKUO YAMAZAKI, MIKIO MIYATA, YOSHITAKA SHINOHARA1969 Volume 2 Issue 2 Pages 61-67
Published: 1969
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA relative content of rhodopsin in outer segment of fresh frozen section of the retina was measured by means of microspectrophotometry with a wavelength at 500nm. A typical process in bleaching and regeneration of rhodopsin could be recorded in situ. Microspectrophotometry was further adapted to study activities of lactate and succinate NBT-reductase system in a fresh frozen section. Courses of reduction of NBT during histochemical procedures could be recorded in situ. This method bridges the gap between histochemistry and enzymology in the fresh cells, especially regarding fundamental energy-linked cell reactions.View full abstractDownload PDF (10865K) -
YOICHIRO TAKASHIMA1969 Volume 2 Issue 2 Pages 68-73
Published: 1969
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe ultrastructure of cortical granules in sea urchin eggs (Hemicentrotus pulcherrimus) was investigated electron microscopically following block staining with phosphotungstic acid (PTA).
The lamellar substance of the components of cortical granules showed intensive affinity for PTA, while non-lamellar substance was not stained. The origin and chemical components of the cortical granules were discussed from the point of their affinity for PTA.View full abstractDownload PDF (6138K) -
SHOHEI YAMASHINA1969 Volume 2 Issue 2 Pages 74-82
Published: 1969
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSSince hydrogen peroxide is produced from glucose oxidized by glucose oxidase, the histochemical localization of glucose can be demonstrated at the electron microscopic level by capturing this hydrogen peroxide. Iron ions show good results as capture reagents.
Cytochemical localization of glucose in mice liver and kidney are observed as fine precipitates in the periphery of glycogen area, rough endoplasmic reticulum and others. These evidences agree well with the localization of glucose-6-phosphatase activity. In the kidney of mice, a lot of deposits of reaction products can be seen in the basal infolding membrane of epithelial cells of convoluted tubules and less deposits in the microvilli, and others.
These findings are thought to be in good agreement with the phosphorylationdephosphorylation theory for the reabsorption of glucose.View full abstractDownload PDF (15954K)
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