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Volume 20, Issue 1
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BUNSUKE OSOGOE, MASAKO YANAGI1987 Volume 20 Issue 1 Pages 1-8
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSIn the present experiments, an attempt was made to use [14C]adenine ([14C]A) for autoradiographic labeling of blood monocytes and their precursor cells (monocytic cells). Main results are as follows.
Following 3 daily injections of [14C]A (1μCi/g body weight each) into young adult rats, from 27 to 75% of blood monocytes together with the majority of their precursor cells in the bone marrow became labeled. Moreover, a portion (up to 7%) of the labeled blood monocytes had as many silver grains per cell as those of their precursor cells in the bone marrow, such as promonocytes or monoblasts.
The former findings, particularly the labeling indices of blood monocytes in the case of [14C]A labeling are almost the same as those in the case of [3H]TdR labeling reported by other workers. However, the latter finding, that is, the abundant occurrence of immature monocytes comparable to promonocytes or monoblasts in the circulating blood has not hitherto been reported by any worker who used [3H]TdR. This indicates that [14C]A is more effective for labeling monocytic cells than is [3H]TdR.
By treatments with RNase or DNase or both, it was confirmed that the observed labeling of monocytic cells with [14C]A was chiefly due to the incorporation of labeled adenine into DNA.
From the biochemical view point, it was suggested that monocytic cells have a limited capacity for de novo adenine synthesis and because of this [14C]A is especially effective for labeling these cells.View full abstractDownload PDF (3726K) -
JYOJI SHIMIZU, SUSUMU IKEHARA, JUNKO TOKI, HITOSHI OHTSUKI, RYOJI YASU ...1987 Volume 20 Issue 1 Pages 9-19
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSNon-obese diabetic (NOD) mice were recently established as a model for type I diabetes mellitus. Immunohistochemical study revealed that Thy-1+ cells (most of the cells are Lyt 1+ but some are Lyt 2+) infiltrate pancreatic islets and selectively destroy beta cells, resulting in overt diabetes. Immunoelectron microscopical study demonstrated the contact of both Lyt 1+ and Lyt 2+ cells with beta cells. A close adhesion of Lyt 2+ cells to beta cells was regularly observed, and pseudopodia-like protrusions of the organella-rich Lyt 2+ cells into the beta cells, resulting in the degeneration of those cells, were frequently seen. By contrast, Lyt 1+ cells were merely in loose contact with beta cells. These results suggest that Lyt 2+ cells are involved in the direct destruction of beta cells in NOD mice.View full abstractDownload PDF (17031K) -
TATSUO SUGANUMA, KAORI IHIDA, SHUNJI MATSUNAGA, SHINICHIRO TSUYAMA, TA ...1987 Volume 20 Issue 1 Pages 21-30
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA biopsy case of a 40-year old housewife, who was admitted to the hospital for bilateral elbow joint pain is examined. Light and electron microscopic examination of the biopsied material from the cystic lesion of her right humerus revealed a membranocystic lesion which was characteristic of membranous lipodystrophy (21).
Histochemical studies using lectin-HRP conjugates provided precise information on the carbohydrate components of the membranocystic lesion. MPA, specific for α-D-galactose residues, strongly stained the membrane of the membranocystic lesion, whereas, RCA-I and SBA, specific for β-D-galactose residues, did not bind the membrane. WGA and HPA failed to stain the membrane of the typical membranocystic lesion, but bound the degenerated adipose cells of the bone marrow.
The morphogenesis and the glycoconjugates histochemistry of the membranocystic lesion are discussed in the present paper.View full abstractDownload PDF (13511K) -
YASUNOBU TOMIDA, HIROSHI KIMURA1987 Volume 20 Issue 1 Pages 31-40
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSTaurine-like immunoreactivity (TLI) in the brain was studied by immunohistochemical and biochemical approaches. The immunohistochemical examination, using different fixatives or taurine antisera preabsorbed with crossreactive substances, demonstrated that TLI staining in the striatum differed from that in the cerebellum. The results suggested that TLI staining should be attributed to unknown substances as well as the amino acid taurine. Immunohistochemical results using a new antiserum raised against γ-glutamyl-taurine supported this assumption. Furthermore, we attempted to extract TLI-positive materials from bovine brain by ion-exchange chromatography and high performance liquid chromatography. A novel immunoreactive substance was isolated and it was distinct immunochemically from either taurine, γ-glutamyl-taurine or glycyl-taurine.View full abstractDownload PDF (7011K) -
WITH SPECIAL NOTES ON GIANT CELLSKEIICHIRO OKAJIMA1987 Volume 20 Issue 1 Pages 41-56
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSImmunohistochemical and biochemical investigations were carried out on alkaline phosphatase (ALPase) in 139 patients with bone tumors and tumorous conditions.
Biochemically, preoperative serum ALPase level was significantly higher in osteosarcoma and chondrosarcoma, and was significantly reduced postoperatively in osteosarcoma. The ALPase activity in the tumor tissue extract was correlated with the serum ALPase level. All these isoenzymes were considered to be bone type ALPases (ALP3).
Liver/bone type ALPase immunostaining was carried out to clarify the following points: 1) Localization of ALPase antigen, 2) differences in stainability between giant cells and small mononuclear cells and 3) relationship of ALPase with calcification by double staining with von Kossa stain. The results were similar to those of enzyme-histochemistry except that stromal cells in the giant cell tumor of bone (GCT) and enchondroma cells were positive for ALPase. Tumorous giant cells were positive in osteosarcoma, but not in GCT and chondroblastoma. Non-tumorous giant cells were all negative. The staining pattern was different between giant cells and stromal cells in GCT, suggesting differences in the origin, differentiation, or intracellular condition between these cells. Differences were present between calcification mediated by osteoid and that in cartilaginous tumors.View full abstractDownload PDF (15675K) -
SHUBH NARAIN, KAZUSHI FUJIMOTO, KAZUO OGAWA1987 Volume 20 Issue 1 Pages 57-73
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSAn ultrastructural study of Ca2+-ATPase activity was performed in the developing parietal cells of the gastric glands of rat utilizing the method of Ando et al. (1981). The enzyme activity was observed from the very beginning of the development of the parietal cells and it was mainly confirmed to the basolateral membranes of the cells in all the stages studied by us. When glutaraldehyde was omitted from the prefixatives, Ca2+-ATPase activity was also detected in the matrix of the mitochondria. There was no marked variation in the localization of the enzyme activity in any developmental period. These findings suggest the possible involvement of Ca2+-ATPase in the maintenance of intracellular Ca2+ concentrations in the gastric parietal cells.View full abstractDownload PDF (32775K) -
MORIMASA MATSUTA, TERUO KAGABU, IWAO NISHIYA1987 Volume 20 Issue 1 Pages 75-85
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSImmunohistochemical localization of Tumor Antigen 4 (TA-4) in squamous cell carcinoma (SCC) tissues of the uterine cervix was studied by means of the immunoperoxidase methods. Light microscopic detection of TA-4 was carried out by means of the avidin-biotin-peroxidase complex method on formalin-fixed paraffin-embedded sections from 92 cases of invasive cervical cancer of the uterus. These tumors were histologically SCC, consisting of 68 of the large cell non-keratinizing (LNK) type and 24 of the keratinizing (K) type. TA-4 positive cells were detected in 65% of LNK cases and 100% of K cases. Positively stained neoplastic cells were seen frequently around the hyperparakeratotic lesions, and they showed a tendency to keratinization.
TA-4 localization was also confirmed in the cells of stratum spinosum of the non-cancerous squamous epithelium.
Subcellular localization of TA-4 was studied by the preembedding indirect immunoperoxidase method. TA-4 was localized in the cytosols of the neo-plastic cells, but not in tonofilaments. It was suggested that TA-4 was a structural protein of SCC. Therefore, unlike keratin, TA-4 can be considered as an index of differentiation of SCC cells, and TA-4 should be useful as a tumor marker which expresses the characteristics of the neoplastic tissue during squamous maturation.View full abstractDownload PDF (18003K) -
KOUNOSUKE MIZUTANI, HIROSHI KIMURA1987 Volume 20 Issue 1 Pages 87-99
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSImmunohistochemistry using an antiserum to fibronectin (FN) demonstrated that FN appeared in neuronal and glial cells in the adult rat brain following cortical injury, while it was absent in neural components of intact rat brain. At early stages of lesioning induced by either cortical undercut or UV irradiation, FN-like immunoreactivity was clearly observed in cytoplasm of damaged neuronal and glial cells. At later stages, FN immunoreactivity was found in some neuronal pathways descending from the lesioned cortex. Immunoelectron microscopic examination confirmed that FN-like immunoreactivity was localized in deformed, probably regenerating, axons and nerve terminals. These results suggest that FN has important roles in scavenging degenerative axonal debris, elongation of regenerating axons, and synaptogenesis.View full abstractDownload PDF (12215K) -
NAOKI ARIZONO, OSAMU KORETO, YASUHIRO IWAI, RYOJI KUSHIMA, SUSUMU NAKA ...1987 Volume 20 Issue 1 Pages 101-104
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSDownload PDF (2192K) -
TERUHIKO OKADA, TOSHIHIRO KOBAYASHI, KAZUO HAKOI, HARUMICHI SEGUCHI1987 Volume 20 Issue 1 Pages 105-110
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSDownload PDF (5569K)
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