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Volume 20 (1987)
- Issue 6 Pages 589-
- Issue 5 Pages 471-
- Issue 4 Pages 375-
- Issue 3 Pages 273-
- Issue 2 Pages 113-
- Issue 1 Pages 1-
Volume 20, Issue 2
Displaying 1-13 of 13 articles from this issue
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YUKIHIRO TATEMOTO, SHUNSUKE KUMASA, YOSHIHISA WATANABE, MASAHIKO MORI1987 Volume 20 Issue 2 Pages 113-124
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA total of 154 cases of salivary gland tumors were studied by an indirect method with the use of monoclonal antibody to epithelial membrane antigen (EMA) from human milk fat globule membrane. Normal salivary glands displayed positive reaction of EMA staining in the luminal and lateral borders of serous acinar cells, and in the luminal border of ducts. Immunohistochemical expression of EMA in salivary gland tumors was chiefly classified into two types: one is the luminal surface-positive type and the other is the whole cellpositive type. Pleomorphic adenoma was usually positive for EMA staining in luminal borders of tubular, duct-like and cystic structures, and occasionally positive in the cytoplasm of the cells comprising these structures. Warthin's tumor was positive for EMA staining on the luminal surface of eosinophilic tumor epithelial cells, or EMA-positive reaction was evident in various areas scattered throughout the epithelial structures. Acinic cell tumor, carcinoma in pleomorphic adenoma (carcinoma cell), and mucoepidermoid tumor showed positive reaction of EMA staining throughout their cytoplasm depending on histological malignancy. No positive reaction to EMA staining was given by either normal myoepithelial cells, and transformed or neoplastic myoepithelial cells.View full abstractDownload PDF (15655K) -
TAKASHI KIMURA1987 Volume 20 Issue 2 Pages 125-133
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe cytochemical localization of glucose-6-phosphatase (G6Pase) activity was examined in human peripheral blood cells and in hematopoietic cells in bone marrow. The reaction product for G6Pase activity was present in the endoplasmic reticulum and nuclear envelope in all types of blood cells except for orthochromatic erythroblasts and mature erythrocytes. In erythroblasts, the reaction product decreased during development from erythroblasts into polychromatophilic erythroblasts. In neutrophil precursors, the reaction product decreased with maturation. Therefore, the maturational stage of hematopoietic cells can be determined according to the relative amount of reaction product for G6Pase activity. The high activity in immature cells may be related to their powerful proliferation. The physiological role of G6Pase in blood cells may be to regulate the concentration of glucose-6-phosphate in the cells as in other cell types.View full abstractDownload PDF (11801K) -
HIROSHI SUMII, SEKIKO WATANABE, TAKUZO ODA1987 Volume 20 Issue 2 Pages 135-146
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSDirect immunoperoxidase staining was applied to the detection of adult T-cell leukemia (ATL)-associated antigens (ATLA) in human T-cell leukemia virus (HTLV-I)-producing MT-2 cells. We successfully conjugated Fab' to peroxidase using a maleimide compound to perform highly sensitive immunohistochemistry with a low background and specific binding. Antisera from three healthy carriers containing antibodies reacting with ATLA were analyzed by Western blotting. ATLA was observed at the light and electron microscopic levels. Positive staining of ATLA was demonstrated in the plasma membrane, endoplasmic reticulum, nuclear membrane, and viral particles, but not in the nucleus nor the mitochondria in frozen-sectioned specimens of MT-2 cells fixed with periodate-lysine-paraformaldehyde (PLP) fixative. Control cells (Molt-4) were not stained. The staining in MT-2 cells and HTLV-I represents the distribution of the viral antigens or their precursors.View full abstractDownload PDF (16514K) -
PAISAL PONGCHAIRERKS, NORIO MATSUKURA, AKIRA TOKUNAGA, NORITAKE TANAKA ...1987 Volume 20 Issue 2 Pages 147-161
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSIn order to find out which type of intestinal metaplasia, complete or incomplete, in the human stomach is more closely related to the small or large intestinal mucosa from the viewpoint of their glycoprotein content, we studied the lectin staining characteristics of the secreted mucin, luminal surface, apical and supranuclear cytoplasm, goblet mucin of both types of metaplastic glands and compared them with those of the small intestinal, ascending and descending colonic mucosa. Three types of lectins, Ulex europaeus agglutinin-I, Dolichos biflorus agglutinin (DBA) and peanut agglutinin, were used with and without prior neuraminidase digestion. Specimens were selected from 32 resected stomachs which contained both metaplastic and non-metaplastic glands. Six specimens were selected from the small intestine and 12 each from the ascending and descending colon.
Blood groups of the patients were also taken into consideration. Findings showed that both types of metaplastic glands possessed definitely different lectin binding properties from the non-metaplastic glands. They also differed from the small and large intestinal mucosa. However, no distinguishable difference was found between the lectin staining of the complete and incomplete types of metaplastic glands. When DBA, a group A antigen-specific lectin was used, patients with blood groups A and AB showed different lectin staining from patients with blood groups B and O. Also, goblet mucin of the metaplastic glands and small intestinal mucosa showed stepwise changes in lectin staining from the bottom to the upper portion of the crypts in accordance with the migration of these cells from the bottom of the crypts upwards.View full abstractDownload PDF (12793K) -
TOSHIKO KOMATSU, SHUJI YAMASHITA, KENJIRO YASUDA1987 Volume 20 Issue 2 Pages 163-176
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA monoclonal antibody against rabbit kidney, γ-GTP, was prepared. The subclass of the antibody was IgG2a and the light chain was λ. The antibody specifically recognized the H chain, the site involved in anchoring part of this enzyme. The immunohistochemical assay detected reaction in the brush border of the proximal convoluted tubule of the rabbit kidney, particularly on the distal part. Cross-reaction between homologous and heterologous organs was immunohistochemically examined using this antibody. In the homologous organs, reaction was absorbed in the intercalated duct of the rabbit pancreas and along the stereocilia of the ductus epididymidis. No cross-reaction was detected in the kidney, pancreas or epididymis of a rat, or in the human kidney.View full abstractDownload PDF (17526K) -
DANIELA AMENTA, CARLO CAVALLOTTI, WADE L. COLLIER, MARCO DE ROSSI, ALB ...1987 Volume 20 Issue 2 Pages 177-184
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe noradrenergic innervation of the superior mesenteric artery of control and 6- or 40-week streptozotocin-diabetic rats was examined by assaying tissue norepinephrine levels and by using the glyoxylic acid histofluorescence technique associated with quantitative image analysis.
Short-term (6-week) experimental diabetes had no significant effect on norepinephrine levels nor on the density of the network of fluorescent nerve fibers that supply the superior mesenteric artery. Long-term (40-week) experimental diabetes was accompanied by a significant reduction of nor-epinephrine content of the superior mesenteric artery and by an impaired morphology of the perivascular plexus of fluorescent nerve fibers.
The present study suggests that long-term streptozotocin diabetes in the rat may be accompanied by neuropathy of the noradrenergic nerves that supply the superior mesenteric artery. The results are discussed in relation to the possibility that, at least in part, the impairment of cardiovascular functions described in human diabetes may depend on alterations of the noradrenergic innervation of blood vessels.View full abstractDownload PDF (5399K) -
KAZU SHIGE OGAWA, KAZUSHI FUJIMOTO, KAZUO OGAWA1987 Volume 20 Issue 2 Pages 185-195
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSAdenylate cyclase (ACLase) and guanylate cyclase (GCLase) were studied in the gastric parietal cells of guinea pigs fed ad libitum and/or starved using the recently improved cytochemical method introduced by Fujimoto et al. (1981).
Generally, GCLase activity was intense in comparison with ACLase activity in the parietal cell. In both, acid-secreting parietal cell and nonsecreting parietal cell, the reaction products indicating both ACLase activity and GCLase activity were recognized on the cytoplasmic side of the basal and lateral membranes, and the lateral apical membrane showing the cell junction with a prominent tight junction. The activity on the basal membrane was stronger than that on the lateral membrane. There was no difference in the intensity of cyclases' activity between the acid-secreting parietal cell and the non-secreting parietal cell in general. GCLase activity was also observed on some tubulovesicles in the non-secreting parietal cell.
These findings suggest that the hormone-sensitive cyclase system is mainly localized on the basal membrane of the cell and that the tight junction of the parietal cell is regarded as the specialized junction.View full abstractDownload PDF (14402K) -
KAZU SHIGE OGAWA, KAZUSHI FUJIMOTO, KAZUO OGAWA1987 Volume 20 Issue 2 Pages 197-216
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSCa++-activated adenosine triphosphatase (Ca++-ATPase), ouabain-insensitive, K+-dependent p-nitrophenylphosphatase (K+-NPPase), a component of H+, K+-ATPase system, and ouabain-sensitive K+-NPPase, a component of Na+, K+-ATPase system, were studied in the gastric parietal cells of guinea pigs fed ad libitum and/or starved using the recently improved cytochemical methods introduced by Ando et al. (1981), Fujimoto et al. (1986) and Mayahara et al. (1980), respectively.
Ca++-ATPase activity was localized: a) on the exterior side of basal and lateral membranes, b) on the interior side of the membrane covering some tubulovesicles, c) just on the membrane covering Golgi apparatus and d) on the matrix of mitochondria. Ca++-ATPase activity on the mitochondria indicated the varying degrees of the intensity in the Ca++ concentration of the reaction medium and in a secretory state of parietal cell. Ca++-ATPase-positive tubulovesicles were not observed in the typical, acid-secreting, parietal cell. H+, K+-ATPase (ouabain-insensitive K+-NPPase) activity was recognized on the cytoplasmic side of: a) the apical plasma membrane, b) the secretory canalicular membrane, c) the tubulovesicular membrane and d) the lateral apical membrane showing the cell junction in both the acid-secreting and nonsecreting parietal cells. H+, K+-ATPase-negative tubulovesicles were also observed. On the other hand, Na+, K+-ATPase (ouabain-sensitive K+-NPPase) activity was observed on the cytoplasmic side of the basal and lateral membranes in both the acid-secreting and non-secreting parietal cells. Na+, K+-ATPase activity was also recognized on a few tubulovesicles of the non-secreting parietal cell.
These findings indicate that: a) the cytochemical properties of the membranes are different between the apical surface, secretory canaliculus, the tubulovesicles and basal-lateral plasma membrane corresponding to the function and b) the tubulovesicles are not uniform in nature.View full abstractDownload PDF (24474K) -
YUJI HORIGUCHI, FUKUMI FURUKAWA, HIDEO KANAUCHI, SADAO IMAMURA1987 Volume 20 Issue 2 Pages 217-227
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSImmunoglobulin (Ig) deposits in the glomeruli of the kidney, lesional skin, upper esophagus and lower cervical part of the uterus of MRL/l mice were comparatively studied by immunoelectron and plain electron microscopy. The massive reaction products in immunoelectron microscopy indicating Ig deposits in the glomeruli of the kidney were observed as electron-optically dense masses by plain electron microscopy. In the skin, esophagus and uterus of the mice, the granules or small masses of reaction products were distributed along the basement membrane zone in association with pathologically increased or normal filamentous constituents such as collagen fibrils, anchoring fibrils, basal lamina or basal lamina-like material and intradermal hemidesmosome-particles. However, plain electron microscopy revealed no obviously pathologic granules or masses in association with the filaments in these organs. These results suggest the possibility that the Ig deposits in the skin, esophagus and the uterus of the MRL/l mice are different in quality and formed in a manner different from those of the glomeruli of the kidney.View full abstractDownload PDF (20997K) -
PAUL K. NAKANE, TETSUYA MORIUCHI, TAKEHIKO KOJI, MASASHI TANNO, KAORU ...1987 Volume 20 Issue 2 Pages 229-243
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSDNA labeled with non-radioactive markers have been used for hybridization with specific DNA or RNA either on filter or in cells and tissues. The presence of protruding markers on the probe DNA has been attributed to the cause for the loss of sensitivity and specificity of hybridization. In search of a non-protruding marker, we investigated the possibility of use of T-T dimer which can be generated easily in DNA and is a potent hapten as a marker for DNA. T-T dimer in DNA was generated by UV irradiation (254μm) for total of 4, 000-5, 000 joules/m2. For in situ hybridization; cells or tissues were first fixed either with Carnoy's fixative or formaldehyde and were usually treated with 0.2N HCl, then hybridized with the T-T dimerized DNA (T-T DNA). The hybridized T-T DNA was detected immunohistochemically using rabbit anti T-T DNA and peroxidase-labeled goat anti-rabbit IgG. With the Southern dot hybridization, the presence of 1 or less pg of complementary DNA can be detected. In cells and tissues, mRNA such as c-myc mRNA and growth hormone mRNA, and various viral DNA mRNA could be localized. The use of T-T as marker offers several advantages over other markers, it appears not interfere with the hybridization efficiency, simple to make and can be detected with high sensitivity.View full abstractDownload PDF (18779K) -
CHIZUKO YANAIHARA, TOHRU MOCHIZUKI, TAKASHI INOUE, KAZUAKI IGUCHI, MIC ...1987 Volume 20 Issue 2 Pages 245-250
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSBased on the predicted amino acid sequence of human placental insulin receptor protein (HIRP), we prepared five synthetic peptides corresponding to purposely selected regions of HIRP; (9-25), (30-61), (48-71), (736-760) and (1139-1171). Antisera were generated against the respective synthetic peptides. Radioimmunoassays with anti-HIRP (30-61) and anti-HIRP (1139-1171) sera could recognize two forms, 270K and 850K daltons, of solubilized insulin receptor in gel filtration fractions, presumably HIRP αβ-monomeric and its tetrameric forms. Immunostaining with the five antisera against the synthetic HIRP fragments revealed positive cells in human placental and hepatic tissues; particularly the antisera stained strongly the membrane regions of Hofbauer cells in the placenta. The results support the validity and usefulness of the region-specific anti-synthetic HIRP fragment sera in studies on the molecular basis of insulin receptor.View full abstractDownload PDF (6541K) -
SONOKO HABU, TAKEHIKO KOJI, AKIRA AKATSUKA1987 Volume 20 Issue 2 Pages 251-260
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSUpon activation with antigens or lectins, a transient expression of mature T lymphocytes cell surface molecules specifically binding to interleukin-2 (IL-2), one of the growth factors, is induced (2, 5, 20, 23). The molecules are designated as interleukin-2 receptors (IL-2R). Interaction of the receptor with IL-2 provides an universal signal for T cell proliferation, resulting in clonal expansion of the activated T cells. In the physiological condition without specific antigen stimulation, proliferation of lymphocytes is prominent in the thymus, particularly in the embryonic thymus, in which we have previously found IL-2R expression of immature lymphocytes (4, 10, 22). Is the IL-2R expressing lymphocytes responsible for the cell proliferation in the thymus as is for the peripheral T lymphocytes? Based on the finding that during the period of gestation, the proportion of IL-2R positive (IL-2R+) cells in the thymus decreases but the cell number of IL-2R+ cells moderately increases, one may postulate that the IL-2R+ cells may be slow growing lymphocytes. Alternatively, it may be postulated that the IL-2R+ cells die in the thymus (27) and that the IL-2R expression of newly arrived lymphocytes, which is induced immediately after their migration into the thymus, contributes to the increase in the number of IL-2R+ cells in vivo. In this paper, proliferation of IL-2R+ cells within the embryonic thymus was examined in the organ culture system in which the mouse embryonic thymus lobes were cultured on a floating membrane to prevent cell migration from extrathymic sites.View full abstractDownload PDF (6601K) -
RICHARD G. WOODBURY, HAI LE TRONG, HANS NEURATH1987 Volume 20 Issue 2 Pages 261-269
Published: 1987
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe properties of the rat mast cell proteases, RMCP I and RMCP II are compared. How the structures of these enzymes may relate to substrate specificity and function is discussed. Additionally, it is proposed that the mast cell proteases represent the best characterized members of a distinct subclass of serine proteases that also include proteolytic enzymes found in cytotoxic lymphocytes and granulocytes.View full abstractDownload PDF (959K)
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