Journal issue
Volume 20 (1987)
- Issue 6 Pages 589-
- Issue 5 Pages 471-
- Issue 4 Pages 375-
- Issue 3 Pages 273-
- Issue 2 Pages 113-
- Issue 1 Pages 1-
Volume 20
, Issue 3
Showing 1-8 articles out of 8 articles from the selected issue
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BUNSUKE OSOGOE, MASAKO YANAGIVolume 20 (1987) Issue 3 Pages 273-281
Released: October 28, 2009
JOURNALS FREE ACCESSAdenine uptake by rat peritoneal macrophages (PM) in vitro was studied by the autoradiographic technique using [14C]adenine ([14C]A), [3H]adenine ([3H]A) and, as control, [3H]thymidine ([3H]TdR).
It was found that the incorporation of either [14C]A or [3H]A by the major population of PM took place to a great extent in contrast to no uptake by peritoneal leucocytes other than PM. In the case of [14C]A labeling, the percentages of labeled PM were 96±3.3 and the mean grain counts per cell were 25±7.6. In the case of [3H]A, the corresponding figures were 83±7.5 and 16±4.1, respectively. Since the label of the major population of PM disappeared almost completely following treatment with RNase, it is apparent that adenine was incorporated into the RNA of these cells. In addition to RNA labeling, an extraordinarily strong labeling of DNA with either [3H]TdR, [14C]A or [3H]A was observed in a limited number of blast-like cells (less than 1% of PM). Between such blast-like cells and the major population of PM, however, there occurred no transitional forms. This indicates that the blast-like cells do not serve as the stem cells for PM under the conditions of this study.
In view of our previous observations that the incorporation of [14C]A into DNA and RNA, the former in particular, takes place to an especially great extent in the monocyte precursors in the bone marrow, also in a portion of the blood monocytes of rats (9, 11, 12), a strong adenine requirement of PM, that was observed in the present study, though limited to that for RNA synthesis, supports the view that PM are derived from monocytes produced in the bone marrow.View full abstractDownload PDF (7832K) -
SHIGEKI MATSUBARA, TARO TAMADA, TAKUMA SAITOVolume 20 (1987) Issue 3 Pages 283-294
Released: October 28, 2009
JOURNALS FREE ACCESSThere were controversial data concerning the localizations of alkaline phosphatase (ALP) and acid phosphatase (ACP) in the human term placenta. These enzymes were reinvestigated histochemically using the lead method. The recently developed cerium method was also employed.
Intense ALP reaction was observed on the plasma membrane of the microvilli at the luminal surface of the syncytiotrophoblasts. Moderate activity of this enzyme was also observed on the basal plasma membrane of the syncytiotrophoblasts and on the plasma membrane of the cytotrophoblasts. The ALP activity was markedly suppressed in a medium containing the enzyme inhibitors, i.e. 1.0mM bromotetramisole or 30mM L-phenylalanine. However, the preheating treatment (65°C, 30min) did not affect the activity of ALP. The ACP reaction products were recognized on the lysosomes and on the Golgi apparatus in the syncytiotrophoblasts as well as in the cytotrophoblasts. Both the lead method and the cerium method yielded consistent results.
As ALP and ACP are fundamental enzymes in enzyme histochemistry, these results might help to investigate the localizations of other specific enzymes, such as Na+/K+ATPase, Ca++ATPase or 5′-nucleotidase, in the human term placenta.View full abstractDownload PDF (12937K) -
IKUO K. TAKEUCHIVolume 20 (1987) Issue 3 Pages 295-304
Released: October 28, 2009
JOURNALS FREE ACCESSThe electron microscopic bismuth staining method of Locke and Huie (16) was applied to the nucleoli of dictyate-stage growing mouse oocytes. After bismuth staining following glutaraldehyde fixation (GA-Bi staining), bismuth was largely localized in the fibrillar centers and the adjoining zones of dense fibrillar components not only in the nucleoli of growing oocytes during the unilaminar, bilaminar and plurilaminar follicle stages, but also in the nucleoli of mature antral-follicular oocytes. These results suggest that the materials stained with the GA-Bi method in the nucleoli of mouse oocytes may have no direct correlation with the transcriptional activity of ribosomal DNA.
In the nucleoli of antral follicular oocytes, the large spherical bodies, consisting of condensed fibrillar components, were unstained with GA-Bi staining but densely stained with bismuth following formaldehyde fixation (FA-Bi staining). This fact suggested the presence of nuclear basic proteins such as histones and protamines in these nucleolar bodies.View full abstractDownload PDF (14730K) -
HIDEKI KUDO, SHINOBU SAKAMOTO, YOSHIE SUGIURA, TOHRU KAWASAKI, TOHRU N ...Volume 20 (1987) Issue 3 Pages 305-313
Released: October 28, 2009
JOURNALS FREE ACCESSWe investigated the effects of testosterone propionate (T) and/or estradiol (E2) on the activity of thymidine kinase (TK) and its isozymes and the incorporation of 3H-thymidine in the prostate of immature rats. The maximum activity of prostatic TK increased approximately 50 times the basal level 48hr after T administration. Puromycin and actinomycin D inhibited nearly 100% of the increase induced by T administration. The prostatic TK isozymes were separated into 3 types by DEAE-cellulose column chromatography, and the activity of the isozyme eluted with buffer alone was markedly increased in T-treated rats, and this isozyme was suggested to be involved in rapid DNA replication because its activity was not affected by deoxycytidine triphosphate (dCTP). The addition of E2 quickened the induction of maximum TK activity due to T administration. Autoradiographic examination indicated that T alone induced marked DNA synthesis in prostatic epithelium, and E2 plus T enhanced DNA synthesis synergistically.View full abstractDownload PDF (4177K) -
FUMIKO TANIMURA, HIROYUKI MORIOKA, MASAYOSHI TACHIBANAVolume 20 (1987) Issue 3 Pages 315-320
Released: October 28, 2009
JOURNALS FREE ACCESSThe middle ear mucosa of guinea pigs was embedded in Lowicryl K4M. Thin sections of the mucosa were stained with a wheat germ agglutinin (WGA)-gold complex and examined by electron microscopy. Intense labeling was observed primarily over the microvillar membranes and the glycocalyx made of a fine filamentous network surrounding the microvilli. However, the labeling was dispersed over the membranes of the cilia and the apical plasma membrane of the epithelial cells. These results indicate that WGA-binding sites are closely associated with the outer structure of the microvilli and their glycocalyceal filaments on the cell surface in the middle ear epithelium.View full abstractDownload PDF (3894K) -
MASANORI MURAKOSHI, KOZOH UENO, YOSHIYUKI OSAMURA, KEIICHI WATANABEVolume 20 (1987) Issue 3 Pages 321-327
Released: October 28, 2009
JOURNALS FREE ACCESSImmunocytochemical localization of glutathione-peroxidase (GSH-PO) in the rat ovary was studied. In ovaries, GSH-PO was clearly detected in granulosa cells of large follicles taken from 2300hr of proestrus to 0500hr of estrus as well as lutein cells taken from midnight of diestrus I to morning of diestrus II. There is a strong correlation between elevated progesterone levels in plasma and immunocytochemical localization of GSH-PO in rat ovarian tissues including granulosa cells and lutein cells. Based on our data, the possible roles of GSH-PO in ovarian tissues were discussed.View full abstractDownload PDF (8043K) -
KEIZO TSURUHARA, NOBUTOSHI KOTANI, TOSHIO NISHIMURA, SOTOKICHI MORIIVolume 20 (1987) Issue 3 Pages 329-336
Released: October 28, 2009
JOURNALS FREE ACCESSDownload PDF (15786K) -
Volume 20 (1987) Issue 3 Pages 339-372
Released: October 28, 2009
JOURNALS FREE ACCESSDownload PDF (22300K)
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