ACTA HISTOCHEMICA ET CYTOCHEMICA
Online ISSN : 1347-5800
Print ISSN : 0044-5991
ISSN-L : 0044-5991
26 巻, 5 号
選択された号の論文の15件中1~15を表示しています
  • TOSHIHISA LEE, NOBUKAZU ARAKI, YOICHIRO TAKASHIMA
    1993 年 26 巻 5 号 p. 337-347
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
    Developmental changes of the bile canaliculi (BC) in rat (fetal 13th-21st day, postnatal and adult) livers were cytochemically examined by fluorescence, light and electron microscopy. Bile canalicular actin filaments (F-actin), consisting of the circumferential pericanalicular F-actin and microvilli (MV) core F-actin, were observed by 7-nitrobenz-2-oxa-1, 3-diazole phallacidin (NBD-ph) fluorescence microscopy and electron microscopy. Mg2+, Ca2+-ATPase activity, which is presumed to be involved in bile acid secretion across the bile canalicular membrane, was detected by a modified Wachstein and Meisel method at both light and electron microscopic levels. Bile canalicular F-actin sparsely appeared from the 13th day of gestation. Since then, the amount of bile canalicular F-actin gradually increased accompanying the bile canalicular formation by 2 weeks after the birth when the development was almost completed. Mg2+, Ca2+-ATPase activity appeared on the fetal 19th day in less than 10% of the BC. The number of Mg2+, Ca2+-ATPasepositive BC rapidly increased around the time of birth (fetal 21st day-postnatal 1st day). The rate of AT-Pase positive BC reached 40-70% during this period. On the 5th day after birth, most BC showed Mg2+, Ca2+-ATPase activity. These findings indicated that the expression of bile canalicular F-actin and ATPase can be regarded as a structural marker and a functional marker for bile secretion, respectively. Structuraldevelopment of BC began earlier but at a rather slower rate than functional maturation.
  • HIROHIKO IMAMURA, YOSHIHIRO AKIMOTO, ICHIRO CHINO, HIROSHI HIRANO
    1993 年 26 巻 5 号 p. 349-358
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
    Focussing on glycoconjugates in the renal corpuscle, we examined changes in lectin binding pattern during fetal and postnatal development of rat kidney by light and electron microscopy using biotinylated lectins as a probe. Lectin binding patterns in the renal corpuscle were divided into 3 groups: 1) positive staining throughout all the developmental stages (WGA, ConA, RCA, DSA, PHA-E, PHA-L, and UEA-I); 2) negative reaction throughout all the developmental stages (PNA, SBA, DBA, GS-II, HPA, Lotus, and AAA); 3) a positive reaction that was detected in embryos, but then disappeared during postnatal development (ECA and UEA-II). After neuraminidase digestion, however, ECA and UEA-II strongly stained the glomerular cells and Bowman's capsule throughout all the developmental stages. Electron microscopically, ECA and UEA-II bindings were intensely positive in the plasma membrane of both podocytes and endothelial cells, and in the glomerular basement membrane. Our results suggest that sialic acid is substituted for fucose and/or added to the terminal residues of glycoconjugates that are specifically recognized by ECA and UEA-II in the plasma membrane of podocytes and endothelial cells, and in the glomerular basement membrane during glomerular development.
  • EIICHI MORII, TATSUMI HIRATA, SEIICHI HIROTA, JUN KOSAKA, HYUNG-MIN KI ...
    1993 年 26 巻 5 号 p. 359-364
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
    Murine cerebellum expresses mRNAs of c-kit and its ligand (Sl factor, SLF). Cell types expressing either SLF or c-kit were investigated using in situ hybridization. Purkinje cells were identified using anti-P400/Inositol 1, 4, 5-triphosphate receptor monoclonal antibody (MoAb). Since all Purkinje, Golgi, basket and stellate cells express mRNA of glutamic acid decarboxylase (GAD), c-kit-expressing cells were identified by comparing their location to that of GAD-expressing cells. SLF mRNA was expressed by Purkinje cells throughout the observation period (day 2 to day 21 after birth). Three types of c-kit-expressing cells developed sequentially in different parts of the cerebellum; Golgi cells on day 2 after birth in the internal granular layer, basket cells on day 6 in the lower half of the molecular layer, and stellate cells on day 14 in the upper half of the molecular layer. The localization of the c-kit protein was also investigated using ACK2 MoAb that recognizes the extracellular domain of the c-kit protein. Basket-like synapses between Purkinje and basket cells, called “pinceau”, were strongly stained with ACK2 MoAb on day 21 after birth. Moreover, the whole molecular layer was stained faintly with the ACK2 MoAb. Since Purkinje cells form synapses between Golgi, basket and stellate cells, SLF and c-kit proteins appeared to play some roles for the synaptogenesis.
  • TAKAHIRO YAMAGUCHI
    1993 年 26 巻 5 号 p. 365-371
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
    In the adenohypophysial cells of swine (S-AHP cells), alkaline phosphatase (ALPase) activity was detected along the plasma membrane and in the cytoplasm. The cells positive for ALPase activity (ALPase cells) were identified as gonadotropic cells by the immunostaining for luteinizing hormone (LH) and follicle-stimulating hormone (FSH). ALPase cells were responsible for the production of both LH and FSH but not for the production of TSH. Ultracytochemically, ALPase cells contained clustered ovoid secretory granules in size ranging from 150 to 400nm. The fine structural organization corresponded to that of swine gonadotropic cells. The product of ALPase activity was localized on the plasma membrane and in the vesicles. The intense activity was detected on the plasma membrane which was engaged in cell to cell contact. In close proximity inside the membrane activity, the endoplasmic reticulum was well developed. The findings suggest that the expression of plasma membrane-associated ALPase may be involved in the interaction between gonadotropic cells.
  • ATSUSHI KATSURA, HISAO YAMADA, JUNZO OCHI
    1993 年 26 巻 5 号 p. 373-379
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
    We ontogenetically examined endothelin (ET) in the lungs and submandibular glands of Wistar rats at various stages, from fetuses to the aged, using specific polyclonal ET-antibody. In the lung, strong ET-immunopositive cells first appeared at 13 days of gestation in the bronchial epithelia, and the strongest immunoreactivity at 17 days; thereafter, the reactivity gradually decreased, disappearing after birth. However, the gene expression of ET-1, ET-3, the receptor A (ET-AR), and B (ET-BR) was observed continuously throughout all the stages. In the submandibular glands, ET-immunopositive cells were first observed in the duct cells at 18 days of gestation; these cells gradually increased in number after birth. In the adult rat, the immunoreactivity was confined predominantly to the granular convoluted tubule. This reactivity was found in every age group, including the aged (3-year-old) rats. All the gene expression of ET-1, ET-3, ET-AR, and ET-BR was observed in the submandibular glands of postnatal and adult rats. We speculate that the ET in these tissues may possibly act as a growth factor.
  • KUNIHIRO URYU, HIROSHI IWATA, SHIGETAKA YOSHIDA, SADAO SHIOSAKA, SEIJI ...
    1993 年 26 巻 5 号 p. 381-389
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
    In situ hybridization histochemistry with the use of a digoxigenin-labeled ribonucleotide probe for basic fibroblast growth factor (bFGF) mRNA demonstrated bFGF transcripts in the masseter muscle of dystrophic (mdx) mouse and in vibrissae and small hair follicle of the rat peri-oral skin. A conspicuous hybridization signal was detected in the central part of the cytoplasm of the smallest myoblasts in the process of initial regeneration or differentiation. bFGF mRNA staining decreased in intensity as the myoblasts increased in size due to the production of myofilaments. Endomysial fibroblasts and extracellular matrix did not exhibit any detectable bFGF mRNA expression. In transverse sections of the hair follicles and vibrissae, a bFGF mRNA positive reaction was noted in the central portion of individual follicles, which were endowed with a non-hybridized core of variable diameter. The hybridization signal in longituidinal sections of hair follicles was localized mainly to keratinizing hair cortical cells; the matrix was scarcely labeled with the probe. The dermal papillae of hair follicles were devoid of bFGF mRNA staining. These findings suggest that early regenerating or differentiating myoblasts produce bFGF, rather than internalizing the growth factor originating in other tissues and that part of bFGF generated in the hair cortical cells is conveyed to the hair matrix and external root sheath which has been shown, by immunohistochemistry, to contain bFGF-like substances.
  • SHIGEHARU KURIMOTO, NOBUO MORIYAMA, YASUSHI NAGASE, NAOTO DOI, YOSHIO ...
    1993 年 26 巻 5 号 p. 391-396
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
    Paraffin-embedded sections of parathyroid adenoma from 27 patients with primary hyperparathyroidism were examined for the expression of proliferating cell nuclear antigen (PCNA). PCNA was expressed on the nuclei of the adenoma cells. The positive rate for PCNA ranged from 0.1% to 2.8% (mean 0.9%). The size of adenomas was not related to the positive rate for PCNA, while the preoperative serum calcium level and positive rate for PCNA revealed statistically the linear regression (y=0.42χ-3.96, p<0.05). Mean level of serum calcium level in the group in which positive rate of PCNA is more than 1.0% is statistically higher than that in the group in which positive rate of PCNA is less than 1.0% (p<0.05).
  • BOGDAN WOZNIEWICZ, JOLANTA KORDOWSKA, PRZEMYSLAW KLUGE, MONIKA PUZIANO ...
    1993 年 26 巻 5 号 p. 397-404
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
    Calcyclin-a calcium binding protein that belongs to the S-100 protein family-is present at high concentration in fibroblasts and epithelial cells. In normal liver calcyclin antibodies stain biliary epithelium, whereas hepatocytes are always negative. Proliferative, degenerative or atrophic changes occurring in biliary epithelium of 8 children with orthotopic liver transplantation (OLT) were monitored using calcyclin antibodies. The antibodies were found to stain epithelium of biliary ducts and ductuli in normal and pathologically changed livers. Single calcyclin positive hepatocytes (probably representing “prebiliary epithelial cells”) could be detected only under pathological conditions.
  • AN IMMUNOHISTOCHEMICAL STUDY
    HIROYUKI OHTSUKA, TOSHIHIKO IWANAGA, MASAYUKI A. FUJINO, TSUNEO FUJITA
    1993 年 26 巻 5 号 p. 405-414
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
    Amylin (Islet Amyloid Polypeptide, IAPP)-containing cells were immunohistochemically investigated in the pancreas and gastrointestinal tract of the rat and humans with reference to its co-existence with gut hormones. Immunoreactivity for amylin was found in pancreatic islets and in endocrine cells dispersed in the pyloric antrum. The major population of islet cells in the rat was immunoreactive for amylin, being identified as B and D cells. Weak immunoreactivity for calcitonin gene-related peptide (CGRP) was recognizable in the rat B cells as reported previously. However it disappeared when the CGRP antiserum was pretreated with amylin, suggesting that the CGRP antiserum might have cross-reacted with an amylin molecule contained in the B cells. Amylin-immunoreactive cells in the pyloric antrum corresponded to gastrin (G) cells in rats and also in humans. This is in contrast to a previous study showing the amylin immunoreactivity in non-G cells of the human pyloric antrum. The amylin immunoreactivity in the pancreatic islets was recognized in rat fetuses, whereas in the pyloric G cells it first appeared on day 28 after birth. Immunohistochemistry at the electron microscopic level demonstrated that amylin was localized in the secretory granules of B, D and G cells.
  • MORIMASA MATSUTA, MAYUMI MATSUTA, KOHSUKE SASAKI, HIDEKI HARIU, IWAO N ...
    1993 年 26 巻 5 号 p. 415-421
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
    Fluorescence in situ hybridization (FISH) with chromosome-specific probes enables several new areas of cytogenetic investigation by allowing visual determination of the presence and normality of specific genetic sequences in single metaphase or interphase cells. However, it is difficult to obtain the characteristic or three-dimensional information of individual DNA sequence in nuclei using the conventional fluorescent microscope. Confocal Laser Scanning Microscope (CLSM) was applied on the touch preparations of ovarian neoplasia and tissue sections of melanoma, which were hybridized with centrometric or c-erb B 2 (HER-2/neu) oncogene-specific probes. It enabled us to observe clear optical sections in vertical or horizontal directions at any depth from the top to bottom of samples and also to reveal the difference of distribution of specific DNA sequences in nuclei using image-analytical devices.
    Copies of chromosome 6 centromeres showed an eccentric localization on optical sections, although those of c-erb B 2 distributed throughout the plans. Furthermore, the reconstructed three-dimensional images revealed that the centromere of chromosome 6 localized close to the nuclear membranes and c-erb B 2 oncogene spread sparsely, or in a dendrometric shape, throughout the nuclei. Since an application of CLSM on the samples which hybridized with DNA sequences specific probes clarified the individual localization of sequences in the nuclei of interphase, this is an especially useful method for biological dosimetry and cancer biology.
  • MIREN P. CAJARAVILLE, JOSE ANTONIO URANGA, EDUARDO ANGULO
    1993 年 26 巻 5 号 p. 423-428
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
    The light microscopical localization of gamma-glutamyl transpeptidase (GGT) was studied in midgut tissues of the mussel Mytilus galloprovincialis Lmk (Molluscs, Bivalvia). The apical portion of the stomach epithelial cells showed an intense positive red coloration, particularly intense in the gastric shield region of the stomach, while the different portions of the intestine were negative. The red granular reaction product was also deposited in non-ciliated cells lining the collecting ducts and in cells lining some digestive tubules. In the latter, the reaction product showed a patchy distribution pattern and was apparently localized within basophilic cells. These results are discussed in connection with the involvement of GGT activity in various important metabolic pathways, specially in the process of extracellular digestion in bivalve molluscs.
  • TATSUKI OYAIZU, HIDEKI TAKAHASHI, HIDETO SENZAKI, YUJI OISHI, AIRO TSU ...
    1993 年 26 巻 5 号 p. 429-433
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
    Antigen retrieval based on microwave-exposure of formalin-fixed, paraffin-embedded tissues in a metal solution of saturated lead thiocyanate or 20% zinc sulfate was investigated. Six leiomyosarcomas and 4 rhabdomyosarcomas were studied using the ABC method applying muscle-related markers. Compared with untreated control sections, vimentin and desmin showed increased staining after the exposure. However, myoglobin immunostaining was not improved. Therefore, some tissue antigens or epitopes were retrieved that were masked by formalin-fixation, but the retrieval was not universal.
  • NAOYUKI KANOH, TAKEO KUMOI, TERUHIKO OKADA, HARUMICHI SEGUCHI
    1993 年 26 巻 5 号 p. 435-439
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
  • NAOTO KAGIYAMA, KIYOHITO YOSHIDA, TAKASHI HAMABATA, NAOTO JUNI, TAKESH ...
    1993 年 26 巻 5 号 p. 441-445
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
  • 1993 年 26 巻 5 号 p. 448-500
    発行日: 1993年
    公開日: 2009/10/28
    ジャーナル フリー
feedback
Top