巻号一覧
28 巻 (1995)
- 6 号 p. 501-
- 5 号 p. 401-
- 4 号 p. 291-
- 3 号 p. 209-
- 2 号 p. 97-
- 1 号 p. 1-
28 巻, 3 号
選択された号の論文の11件中1～11を表示しています
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Method for Obtaining Larger Giant CellsShizuya Saika, Shunsaku Ohmi, Ryuichi Kanagawa, Sai-ichi Tanaka, Yoshi ...1995 年 28 巻 3 号 p. 209-215
発行日: 1995年
公開日: 2010/02/26
ジャーナル フリーWe studied the ultrastructure of giant cells derived from macrophages that are found on the surface of implanted polymethylmethacrylate intraocular lenses (IOLs) in rabbits and the immunolocalization of vimentin in these cells in order to study the rearrangement of the intermediate filaments in multinucleated cells. In adult Japanese albino rabbits, the crystalline lens was extracted and an IOL was inserted into the residual lens capsule. IOLs were removed after 7 days, and the specimens were processed for ultrastructural observation or for immunostaining for vimentin. Macrophages and multinucleated cell were detected on the surfaces of IOLs by both light and transmission electron microscopy. Nuclei of the large multinucleated cells showed a circular distribution with the center of the cytoplasm occupied by cytoplasmic vacuoles. Vimentin intermediate filaments were observed in the cytoplasm of both macrophages and multinucleated cells. Vimentin in giant cells showed a radial distribution. Results indicate that vimentin intermediate filaments become reorganized after the fusion of macrophages. Experimental implantation of an IOL into the animal eye is a useful method for obtaining larger giant cells derived from macrophages.抄録全体を表示PDF形式でダウンロード (10401K) -
Shuji Yamashita1995 年 28 巻 3 号 p. 217-225
発行日: 1995年
公開日: 2010/02/26
ジャーナル フリーLactoferrin (LF) is known as an estrogen-inducible protein in the murine uterus. This study, employing immunoelectron microscopy with the pre-embedding methods, was carried out to elucidate temporal LF induction, the process of the induction and intracellular localization after 17βestradiol (E2) stimulation in the enodometrial epithelium of ovariectomized adult mice. By single i. p. injection of E2 (20μg/kg b. w.), LF was rapidly induced, even after 1 hr, and was exclusively localized in the nucleoli of surface and glandular epithelium. At 7 hr after E2 injection, strong immunostaining was recognized in the amorphous cytoplasm and in nucleoli, especially in the dense fibrillar component of the epithelium. From 13 hr to 23 hr after E2 administration, strong reaction was observed in the secretory pathway, i. e., cisternae of endoplasmic reticulum and the Golgi apparatus, and in vesicles and vacuoles in the apical cytoplasm, in addition to the nucleolar staining. LF-immunoreaction was also detected in the nucleoli and cytoplasm of stromal and muscle cells; it was demonstrated in the epithelium at the earliest period, subsequently in the stromal cells (7 hr), and finally in the muscle cells (13 hr). After three days of consecutive E2 stimulation, many secretory granules in the apical cytoplasm and apical cell membrane showed intense LF immunoreaction. The present study suggests that LF in the nucleolus plays an important role in activation of ribosomal biogenesis preceding the cell differentiation and proliferation in the mouse uterus.抄録全体を表示PDF形式でダウンロード (11098K) -
Thomas Monzy, Jain Sudhir, Kumar G. Pradeep, Laloraya Malini1995 年 28 巻 3 号 p. 227-230
発行日: 1995年
公開日: 2010/02/26
ジャーナル フリーThe process of implantation has been looked upon as an adhesion phenomenon of the blastocyst cells over the endometrial lining, mediated by membrane surface components. Experiments were performed to probe accessible surface thiol groups in the endometrium at various stages of its receptivity. A transient and abrupt“thiol expression”was marked in coincidence with the time of implantation. It is plausible that the adhesion of blastocyst to the uterine epithelium may involve a disulphide bonding between the thiols present on the surface of interacting counterparts.抄録全体を表示PDF形式でダウンロード (4196K) -
Jun-ichi Tanaka, Kayo Mishiro, Jun Watanabe, Shinsuke Kanamura1995 年 28 巻 3 号 p. 231-237
発行日: 1995年
公開日: 2010/02/26
ジャーナル フリーAlthough acetylcholine (Ach) is an important neurotransmitter, there is no adequate histochemical method to detect Ach in tissue sections. We therefore developed a method to visualize the localization of Ach in tissue sections by a combination of immunohistochemistry with ionic fixation. Brains of mice, perfused with 4% formaldehyde and 5% phosphomolybdic acid (PMA) in 0.1 M phosphate buffer (pH 7.4) under sodium pentobarbital anesthesia, were removed and immersed quickly in boiling PMA solution for 5 min. Frozen sections were cut and soaked in a buffer containing 5% PMA at 4°C for 30 min, and immersed in a buffer containing bovine serum albumin at 4°C for 1 hr. Then the sections were incubated with anti-Ach antibody at 4°C for 12 hr. Subsequently, the immunoreaction due to Ach was visualized by the peroxidase-antiperoxidase method. Nerve cells in the brain sections from the interpeduncular nucleus of the cerebrum and bipolar cells in the multiform layer of the cerebral cortex were immmunohistochemically stained with the anti-Ach antibody.抄録全体を表示PDF形式でダウンロード (8746K) -
Suguru Yonezawa, Mitsuharu Nomoto, Sumika Matsukita, Pei-Xiang Xing, I ...1995 年 28 巻 3 号 p. 239-246
発行日: 1995年
公開日: 2010/02/26
ジャーナル フリーWe have previously reported that MUC2 gene product was dominantly expressed in the non-invasive carcinomas of the pancreas and liver (Cancer 71: 2191-2199, 1993; Int J Cancer 55: 82-91, 1993). Mucinous carcinoma (MC) of the breast usually shows less frequent lymph node metastasis and a more favorable outcome compared with invasive ductal carcinoma (IDC). In the present study, we examined the expression of MUC1 gene product (DF3, 139H2) and MUC2 gene product (anti-MRP, CCP58) immunohistochemically in 17 cases of MC (11 pure MCs, and 6 mixed MCs comprising MC and IDC) and 57 cases of IDC. Most of the pure MCs showed MUC1 positive (DF3: 11/11, 139H2: 11/11) and MUC2 positive (antiMRP: 10/11, CCP58: 11/11) pattern. Most of the mixed MCs showed MUC1 positive (DF3: 6/6, 139H2: 6/6) and MUC2 positive (anti-MRP: 5/6, CCP58: 6/6) pattern in the MC areas, but MUC1 positive (DF3: 6/6, 139H2: 6/6) and MUC2 negative (anti-MRP: 0/6, CCP58: 0/6) pattern in the IDC areas. In contrast, most of the IDCs showed MUC1 positive (DF3: 54/57, 139H2: 53/57) and MUC2 negative (anti-MRP: 0/57, CCP58: 0/57) pattern, but never showed MUC1 positive and MUC2 positive pattern. Thus, the expression of MUC2 gene product is associated with the less aggressive biological properties of MC than IDC through the production of abundant extracellular mucin forming the characteristic configuration, since MUC2 glycoprotein is a secretory mucin, while MUC1 glycoprotein is membrane-associated.抄録全体を表示PDF形式でダウンロード (8075K) -
Toshisuke Hiraoka, Kei-ichi Hirai1995 年 28 巻 3 号 p. 247-254
発行日: 1995年
公開日: 2010/02/26
ジャーナル フリーThe platinum diaminobenzidine (Pt-DAB) reaction was characterized by the oxidative polymerization of DAB with a concurrent incorporation of Pt atoms into the polymerization product in the presence of cytochrome oxidase activity. It conformed to the Michaelis-Menten's formula showing its enzymatic nature. It was specific to the oxidase as proved by the suppressive effects due to some inhibitors and heat. It permitted the quantification of the oxidase activity of an individual mitochondrion and its constituent part by energy dispersive X-ray analysis (EDAX) owing to the incorporated Ptatoms. To elucidate the gradient in the oxidase activity along the radial axis of a liver lobule, the local activity difference between the hepatocytes in the peripheral region (PE) adjacent to the triad and those in the central region (CN) close to the central vein was quantified at individual mitochondrial level. The mitochondria in the PE hepatocytes were found 1.5 times stronger in activity per area of the reactive site than those in the CN hepatocytes.抄録全体を表示PDF形式でダウンロード (4362K) -
Yu-Yi Chien, Nai-Shin Chu1995 年 28 巻 3 号 p. 255-261
発行日: 1995年
公開日: 2010/02/26
ジャーナル フリーThe effects of partial and total denervation on muscle fibers were studied in rat soleus muscles by quantitative computer imaging analysis. Within 5 weeks after denervation, there was a progressive reduction of the soleus muscle weight in total denervation groups but a gradual recovery beginning in the third week and reaching 75% of the control in the fifth week in the partial denervation group. The reduction of muscle mass was mainly due to the replacement of large and medium-sized fibers by small atrophic fibers.
In totally denervated muscles, cross-sectional fiber area showed a rapid decline after denervation, but the shape of fiber size distribution was nearly normal. On the other hand, partially denervated muscles showed an asymmetrical fiber size distribution with a shift toward large fibers. In control and totally denervated muscles, mean fiber area strongly correlated with the standard deviation from the mean, but in partially denervated muscles, this strong correlation no longer existed and was replaced by larger standard deviations. It is concluded that only partially denervated muscles show significant variations in fiber size.抄録全体を表示PDF形式でダウンロード (7948K) -
Isamu Sugawara, Masatomo Onodera, Takehiko Koji, Masato Takenaga, Shuj ...1995 年 28 巻 3 号 p. 263-267
発行日: 1995年
公開日: 2010/02/26
ジャーナル フリーThe presence of H. pylori was examined by non-radioactive in situ hybridization using a digoxigenin-labelled DNA probe and a synthetic H. pylori urease gene-specific thymine-thymine dimerized oligonucleotide probe. H. pylori was detected in 20/33 gastric biopsy specimens, and was localized in gastric epithelia and neutrophils. No nonspecific DNA signal was detected in colonic mucosa, esophageal mucosa, skin and liver specimens. The DNA probe did not crosshybridize with DNA from other bacteria. As this method is specific and not time-consuming, it may be useful for revealing the location of H. pylori.抄録全体を表示PDF形式でダウンロード (3370K) -
Toshiya Tomioka1995 年 28 巻 3 号 p. 269-275
発行日: 1995年
公開日: 2010/02/26
ジャーナル フリーThis study evaluates temporal increases in the expression of Fos-like immunoreactive labeled neurons (FLI-labeled neurons) in the spinal trigeminal nucleus complex (STNC) following selective stimulation of C-fibers. Selective stimulating C-fibers were obtained using low doses (10 mg/kg b. w.) of capsaicin injection. Under sodium pentobarbital anaesthesia, 1% capsaicin was administered into the rats left infraorbital region. At periods of 30 min, 1, 3, 6, 9 and 12 hr after capsaicin injection, rats were anesthetized and killed for immunohistochemical examination of Fos-like protein products. The results showed that FLI-labeled neurons in the STNC appeared within 30 min after induction of capsaicin injection into the rat trigeminal region, reached peak increases at 3 hr, and disappeared between 9 and 12 hr. This time course was similar to dental treatment results which probably stimulated both A-delta and C fibers. According to previous studies, both A-delta and C fibers are essential to conduct impulses of nociceptive stimulation. But, this theory does not apply to the time course of FLI-labeled neuron expression in the STNC.
In conclusion, this study showed that the time course of FLI-labeled neuron expression in the STNC was unchanged in stimulated or non-stimulated A-delta fibers.抄録全体を表示PDF形式でダウンロード (4520K) -
Taketo Yamada, Jun-ichi Hata1995 年 28 巻 3 号 p. 277-280
発行日: 1995年
公開日: 2010/02/26
ジャーナル フリーWe have developed a simple and rapid method for detection of apoptotic cells by flow cytometry -a modified nick end-labeling method. DNA fragments in situ associated with apoptosis are detected by the nick-end labeling method (TUNEL method) in our new method. This method is well suited for easy identification of cell types and for in situ measurement of minute quantities of apoptotic cells. We present a modified method for nick-end labeling by flow cytometry (NELF) to detect apoptosis of both cultured cells and blood cells and also to analyze DNA fragments quantitatively and at the single-cell level. We modified the NELF method to simplify procedures and to save time. The protocol consists of 1) fixation with cold acetone, 2) transfer of biotiny lated nucleotide to the 3′-OH end with terminal deoxynucleotidyl transferase, and 3) incubation with streptoavidin-conjugated phycoerythrin to biotinylated nucleotide. This method may be useful in two-color analysis with a wide variety of antibodies.抄録全体を表示PDF形式でダウンロード (1338K) -
Osamu Fukushima, Hiroshi Yamashita1995 年 28 巻 3 号 p. 281-286
発行日: 1995年
公開日: 2010/02/26
ジャーナル フリーThere have been very few papers reporting on the localizations of aminopeptidases and dipeptidyl peptidases activities in bone tissue, although these peptidases seem to have important roles in bone resorption and osteoid degradation. The present study demonstrates these peptidases in fixed and decalcified tibial metaphyses of 3-week-old chickens using the azo-dye methods at light microscopic level. As substrates, amino acid derivatives of 4-methoxy-2-naphthylamine (MNA) were used: Leu- or Ala-MNA for aminopeptidase-M (AP-M, EC 3.4.11.2), Glu-MNA for aminopeptidase-A (AP-A, EC 3.4.11.7), Gly-Arg-MNA for dipeptidyl peptidase-I (DPP-I, EC 3.4.14.1), Lys-Ala-or Lys-Pro-MNA for dipeptidyl peptidase-II (DPP-II, EC 3.4.14.2), and Gly-Pro-MNA for dipeptidyl peptidase-IV (DPP-IV, EC 3.4.14.5). An AP-M in osteoclasts, and APA in osteoblasts and osteocytes were observed. These activities were sensitive to 10 mM of ethylenediaminetetraacetic acid (EDTA). A DPP-I was seen in osteoclasts, osteoblasts and osteocytes in the presence of mercaptoethylamine (MEA), however Gly-Arg-MNA hydrolyzing activity in the absence of MEA was restricted to only osteoclasts. DPP-II activity was present in osteoblasts and osteocytes, using Lys-Pro-MNA as a substrate. There was no Lys-Ala-MNA hydrolyzing activity in bone cells. The present study failed to demonstrate DPP-IV activity in either 4% paraformaldehyde- or 2.5% glutaraldehyde-fixed samples, although it was observed in the glomerulus and proximal convoluted tubule cells of the rat kidney fixed with glutaraldehyde. The present study suggests that AP-M and DPP-I in osteoclasts, and AP-A, DPP-I and DPP-II in osteoblasts seem to function in the bone remodeling process.抄録全体を表示PDF形式でダウンロード (3102K)
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