巻号一覧
29 巻 (1996)
- 4 号 p. 273-
- 3 号 p. 189-
- 2 号 p. 85-
- 1 号 p. 1-
29 巻, 4 号
選択された号の論文の10件中1～10を表示しています
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Eiji Ishikawa1996 年 29 巻 4 号 p. 273-281
発行日: 1996年
公開日: 2010/02/26
ジャーナル フリーUltrasensitive enzyme immunoassays (immune complex transfer enzyme immunoassays) were developed for antibody IgGs. Antibody IgGs were reacted with 2, 4-dinitrophenyl-antigen and antigen-β-D-galactosidase conjugate, and the immune complexes comprising the three components were trapped onto solid phase coated with (anti-2, 4-dinitrophenyl group) IgG and were transferred to solid phase coated with (antihuman IgG γ-chain) IgG in the presence of εN-2, 4-dinitrophenyl-L-lysine. These immunoassays were much more sensitive and specific than currently available conventional methods and were applied for detection of antibody IgGs to HIV-1. Diagnosis and confirmation of HIV-1 infection using urine, whole saliva and serum samples became more reliable with less indeterminate results. And the window period in diagnosis of HIV-1 infection, during which diagnosis of HIV-1 infection is not possible due to the absence of detectable antibodies to HIV-1, was shortened by approximately two weeks with the immune complex transfer enzyme immunoassay for simultaneous detection of p24 antigen of HIV-1 and antibody IgGs to p17 antigen and reverse transcriptase of HIV-1 in a single assay tube. Furthermore, it has recently become possible to perform the immune complex transfer enzyme immunoassay within 2 to 3 hr with higher sensitivity and, as a result, to further shorten the window period in diagnosis of HIV-1 infection.抄録全体を表示PDF形式でダウンロード (2240K) -
Effects of Castration and Administration of TestosteroneMasanori Murakoshi, Rie Ikeda, Masashi Tagawa, Minoru Suzuki, Susumu T ...1996 年 29 巻 4 号 p. 283-288
発行日: 1996年
公開日: 2010/02/26
ジャーナル フリーImmunolocalization of glutathione-peroxidase (GSH-PO), apoptosis and bcl-2 protein in the rat ventral prostate was investigated under the presence or absence of androgen. Male Sprague-Dawley rats were divided into four experimental groups. Group 1 consisted of intact controls. In group 2, rats were sacrificed two days after castration. In groups 3 and 4, rats were subcutaneously administered 1 mg/animal of testosterone-propionate daily for three or seven days after two days of castration. The intensity of GSH-PO staining in the glandular epithelial cells of the ventral prostate was remarkably decreased after castration (Group 2), and it was clearly recovered by testosterone-administration (Groups 3 and 4) to the castrated rats. The prostatic GSH-PO mRNA levels were diminished in the castrated rat ventral prostate but greatly increased by testosterone (Groups 3 and 4). Furthermore, castration (Group 2) induced apoptosis in the prostatic glandular epithelial cells and apoptosis was reduced by testosterone-administration (Groups 3 and 4) to the castrated rats. In groups 3 and 4, expression of bcl-2 protein was clearly detected in the glandular epithelial cells of the ventral prostate. These findings strongly suggest that expression of GSH-PO and bcl-2 protein in the glandular epithelial cells of the rat ventral prostate is considered to be testosterone-dependent.抄録全体を表示PDF形式でダウンロード (7368K) -
Yasusuke Kawada, Kohtaro Asayama, Kazushige Dobashi, Takaya Nakane, Hi ...1996 年 29 巻 4 号 p. 289-297
発行日: 1996年
公開日: 2010/02/26
ジャーナル フリーTo quantitate the developmental changes in copper zinc and manganese superoxide dismutases (CuZnSOD and MnSOD) during the perinatal period, tissue sections were obtained from fetal (day 12 to day 22) and neonatal (day 6) rats, and were stained immunohistochemically using specific polyclonal antisera. The intensity of the staining was quantitated by fluorescence microscopy image analysis. There was a general trend of enriched SOD in the epithelial linings and metabolically active sites. Significant fluorescence was detected in hepatocytes, renal tubular epithelium, bronchial epithelium and intestinal epithelium at fetal day 15. The intensity increased in a stepwise manner thereafter. The fluorescent image for CuZnSOD was 1.6-fold more intense at postnatal day 6 than at fetal day 15 in each tissue studied. Similarly, the overall increase in the intensity of MnSOD staining was 2.0-fold in the liver, kidneys, and lungs, and 1.8-fold in the intestine. The phase of rapid increase for both SOD started at day 19 in most tissues. These results suggest that the intracellular O2--scavenging system develops during the perinatal period preferentially in epithelial tissues as an essential mechanism for living under atmospheric oxygen conditions.抄録全体を表示PDF形式でダウンロード (7710K) -
Li Li, Yu-Xiu Shi, Kazuo Ogawa1996 年 29 巻 4 号 p. 299-303
発行日: 1996年
公開日: 2010/02/26
ジャーナル フリーIn this study, the presence of nematolysosome (NLY) with trimetaphosphatase (TMPase) activity and acid phosphatase (ACPase) activity in the neuron of anterior horn in guinea pig spinal cord was certified by means of enzyme cytochemistry. NLYs with TMPase and ACPase activity were observed in soma, process and synapse, where NLYs attach to mitochondria tightly. The observations we made revealed that NLY could transport from soma to nerve terminal, which chiefly depended on mitochondria to provide energy for the movement of NLYs. Our findings implied that NLYs may be related to the decomposition of neurotransmitters, the disposition of metabolites and the TMPase and ACPase transport in neuron. Meanwhile, the quantification of the TMPase and ACPase activity of NLYs by energy dispersive X-ray analysis (EDAX) which indicated that the activity of TMPase was higher than that of ACPase, completely complied with the observation of cytochemistry that the number of NLYs with TMPase activity was much more than that with ACPase activity. Our results cytochemically and quantitatively showed that as a lysosomal maker enzyme, TMPase is more sensitively demonstrated than ACPase in the neuron of anterior horn in guinea pig spinal cord.抄録全体を表示PDF形式でダウンロード (3015K) -
Kazuto Yamada, Wataru Kudeken, Shinichiro Sumitomo, Yasunori Muramatu, ...1996 年 29 巻 4 号 p. 305-310
発行日: 1996年
公開日: 2010/02/26
ジャーナル フリーE-cadherin is a calcium dependent cell adhesion molecule, important in cell to cell interactions in epithelial tissues. The present study examined E-cadherin expression in normal salivary glands, pleomorphic adenoma, Warthin's tumor, mucoepidermoid carcinoma, acinic cell carcinoma, adenoid cystic carcinoma and adenocarcinoma of salivary glands in paraffin sections using a monoclonal antibody (5H9). This study is firstly reported E-cadherin expression in the salivary gland and tumors.
In normal submandibular and parotid gland, E-cadherin staining was confined to the cell membrane of acinar and ductal cells. In excretory ducts, high columnar cells showed the strongest reaction at the basal aspect and ductal basal cells were weakly positive or negative, the basement membrane zone lacked staining. No E-cadherin reaction was observed in modified myoepithelial cells and plasmacytoid cells. In pleomorphic adenoma and mucoepidermoid carcinoma, E-cadherin was positive at the lateral cell membrane or whole cell membrane of duct-like structure and epidermoid cells. Acinic cell carcinoma was negative for E-cadherin. In adenoid cystic carcinoma, the expression was only found in cribriform areas. In adenocarcinoma, the staining was seen in the cell membrane of duct like structures and occasional cells showed a strong cytoplasmic reaction. Positive E-cadherin staining in salivary gland tumors was higher in benign tumors than in malignant tumors.抄録全体を表示PDF形式でダウンロード (5434K) -
Hidetoshi Ehara, Takashi Deguchi, Takehiko Koji, Muzheng Yang, Shin-ic ...1996 年 29 巻 4 号 p. 311-318
発行日: 1996年
公開日: 2010/02/26
ジャーナル フリーAlthough information on the states of androgen receptor (AR) expression at the individual cell level is essential in understanding the human diseased prostate, histological approaches are often hampered by the artifactual loss of AR or AR antigenicity. Here we examined the effects of various antigen retrieval methods, including microwave irradiation and autoclave treatment, for immunohistochemical detection of AR in cultured LNCaP cells and paraffin embedded sections of human prostate, and found beneficial effects of autoclave treatment over microwave treatment. Staining results were consistent with that of AR messenger RNA expression assessed by the reverse transcriptase-polymerase chain reaction method. The procedures described in this article provided intense and reproducible immunostaining for AR, estrogen receptor and progesterone receptor in paraffin sections of the human diseased prostate. Finally, 0.01M EDTA (pH7.4) gave us the most intense nuclear signal for the steroid hormone receptors, though the best soaking solution was 0.01M citrate buffer (pH6.0) considering the lost of tissue morphology.抄録全体を表示PDF形式でダウンロード (11706K) -
Kazuyoshi Sakai, Nobuyuki Karasawa, Keiki Yamada, Ryouhachi Arai, Ikuk ...1996 年 29 巻 4 号 p. 319-326
発行日: 1996年
公開日: 2010/02/26
ジャーナル フリーThe distribution of monoamine oxidase (MAO) -containing neuronal somata was studied in the grass parakeet (Melopsittacus undulatus) brain by using a histochemical technique involving the coupled peroxidatic oxidation method, and compared with the distribution of the tyrosine hydroxylase-, aromatic L-amino acid decarboxylase-, serotonin-immunoreactive cells in the grass parakeet brain. MAO-containing neurons were located in the nucleus locus coeruleus, around the nucleus n. facialis (nVII), lateral region to the nucleus olivaris inferior and nucleus tractus solitarius, where noradrenaline neurons exist. Another group was located in the ventral region of nervi oculomotorii, caudal region of nucleus n. oculomotorii (nIII), the nucleus raphe and ventral to the nucleus olivaris superior, where serotonin neurons exist. A third group was the non-monoaminergic neurons which were located in the lobus parolfactorius, pareostriatum augmentatum and ventrolateral part of formatio reticularis mesencephali.抄録全体を表示PDF形式でダウンロード (5450K) -
Jun'ichi Shimada, Shinji Fushiki, Katsuhiko Nishiyama, Nobuhide Kobori ...1996 年 29 巻 4 号 p. 327-333
発行日: 1996年
公開日: 2010/02/26
ジャーナル フリーThe vagal innervation pattern supplying viscera such as lungs and gastrointestinal tracts is important, since such visceral efferents are involved in the normal function and some diseases of these organs. We used in vivo injection an alcoholic solution of 0.1% 1.1′-dioctadecyl-3, 3, 3′, 3′-tetramethyl-indocarbocyanine perchlorate (Dil) into the anterior and posterior wall of the antral stomach and the mid portion of the left lung, to quantitatively analyze the distribution of Dil-labeled neurons in the dorsal motor nucleus of the vagus nerve and the nucleus ambiguus of Wistar rats two weeks later. Transverse sections of the brain stem were examined microscopically under epifluorescence with appropriate filters. Neuronal cell bodies and processes in these nuclei revealed particles of Dil. Our observations indicate that the dye can be transported retrograde along long axons when injected into target viscera and demonstrate that Dil is a useful quantitative tool for studying their innervating neurons in the brain stem.抄録全体を表示PDF形式でダウンロード (5645K) -
Sadaki Yokota, Yoshiie Okada1996 年 29 巻 4 号 p. 335-338
発行日: 1996年
公開日: 2010/02/26
ジャーナル フリーEffect of reduced OsO4 fixation on the antigenicity of rat liver catalase and erythrocyte esterase D was investigated. After fixation with mixture of 4% paraformaldehyde and 0.2% glutaraldehyde, rat liver and erythrocytes were post-fixed with reduced osmium (0.1% to 1.0%) for 1 hr. Some erythrocytes were fixed with 0.5% reduced osmium for various times (10 min to 60min). The antigenicity of both enzymes was expressed as labeling density (gold particles/μm2 of peroxisomes or erythrocytes). Fixation with reduced OsO4 greatly affected the antigenicity of both enzymes. Approximately 80% of catalase antigenicity was lost at 0.4% reduced OsO4, whereas about 80% of esterase D antigenicity was lost at 0.2%. Positive contrast of membrane structures clearly appeared at 0.6% OsO4. About 80% loss of the esterase D antigenicity was observed at 10min after fixation with 0.5% reduced OsO4. Afterwards, the antigenicity decreased very slowly. The data indicates that at the concentration of reduced OsO4 which stains the membrane structures positive contrast, 80% of the antigenicity is lost in the cases of catalase and esterase D.抄録全体を表示PDF形式でダウンロード (2729K) -
Eiko Aoki, Reiji Semba, Hu Ying, Ma Ning, Ikuo K. Takeuchi1996 年 29 巻 4 号 p. 339-343
発行日: 1996年
公開日: 2010/02/26
ジャーナル フリーPrenatal and postnatal development of neuronal NO synthase (nNOS) immunoreactivity and NADPH diaphorase (NADPHd) histochemical activity were studied in the rat kidney. NADPHd activity appeared at prenatal day 18, and nNOS immunoreactivity was first detected at prenatal day 19. However, these activities were rarely found before the prenatal day 20, and their staining intensity was very weak. Neuronal NOS protein and NADPHd activity appeared first in the inner layer of renal cortex. In higher magnification, they localized not only in macula densa cells but also in perimacular epithelial cells connected to the macula densa. With development, the number of nNOS immunoreactive and NADPHd positive sites increased dramatically and many nNOS-positive maculae densae were detected in the juxtamedullary region of the cortex at postnatal day 5. At postnatal day 14, the staining pattern of nNOS and NADPHd was similar to that of adults. NADPHd activity and nNOS immunoreactivity were found in very early stages of nephrogenesis including in prenatal days before full development of the glomerulus.抄録全体を表示PDF形式でダウンロード (9171K)
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