ACTA HISTOCHEMICA ET CYTOCHEMICA
Online ISSN : 1347-5800
Print ISSN : 0044-5991
ISSN-L : 0044-5991
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Volume 35, Issue 1
Displaying 1-5 of 5 articles from this issue
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REGULAR ARTICLE
  • Immunohistochemical Studies on Annexin I and II in Takayasu Arteritis
    Miho Ohkawara, Fujio Numano
    Article type:  
    Subject area:  
    2002 Volume 35 Issue 1 Pages 1-10
    Published: 2002
    Released on J-STAGE: April 03, 2002
    DOIhttps://doi.org/10.1267/ahc.35.1
    JOURNAL FREE ACCESS
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    Annexin I and II are classical types of the annexin family and known for their physiological functions in inflammatory processes and thrombus formation. Takayasu arteritis, on the other hand, is a vasculitis of unknown etiology characterized by the inflammatory involvement of the internal vessel wall and thrombus formation. It causes the stenotic and/or obstructive changes of the arterial wall leading to the characteristic clinical feature of pulselessness.
    Expression of annexin I and II in vessel walls obtained from nine patients with Takayasu arteritis was studied immunohistochemically to investigate their roles in this morbid condition. Expression of annexin I was recognized in foamy cells in the thickened intima and/or medial layer and in inflammatory cells in vessel walls, but neither in endothelial cells nor in normal or proliferated smooth muscle cells. Strong expression of annexin II was present in foamy cells as well as in endothelial cells both in thickened intima and in proliferated smooth muscle cells in the intima, but not in smooth muscle cells in the media. The stronger expression of annexin II was seen in the endothelial cells in the vasa vasorum of both thickened and normal adventitia. These results suggest the participation of annexin I and II in the process of this vasculitis, and an essential role of vasa vasorum in the pathogenesis of Takayasu arteritis.
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  • Distribution of Inducible Nitric Oxide Synthase, Interleukin-1β, and Interleukin-1 Receptor in the Temporomandibular Joint of Normal Rats
    Keitaro F. Masuda, Takayoshi Yamaza, Yoshihiro Tsukiyama, Rie Murakami ...
    Article type:  
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    2002 Volume 35 Issue 1 Pages 11-21
    Published: 2002
    Released on J-STAGE: April 03, 2002
    DOIhttps://doi.org/10.1267/ahc.35.11
    JOURNAL FREE ACCESS
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    Nitric oxide (NO) is generated from l-arginine by NO synthase (NOS) and has multiple functions under both physiological and pathological conditions. One isoform of NOS, inducible NOS (iNOS) is expressed in the cells such as macrophages after induction with various cytokines or mechanical stress. In this study, we investigated the distribution of iNOS, interleukin-1β (IL-1β) and its receptor, the IL-1 receptor (IL-1R), in the synovial membrane of the temporomandibular joint (TMJ) of normal rats using immunolight and immunoelectron microscopy. By light microscopy, an immunopositive reaction for iNOS and IL-1β was found in the superficial cells of the synovial membrane of both the anterior and posterior portions of the articular disc. Immunoelectron microscopy revealed that iNOS-immunoreactive products were deposited in the cytoplasm and vesicles, and on the plasma membrane of type-A (macrophage-like) and B (fibroblast-like) cells of the superficial layer. IL-1R-positive products were found both on the plasma membrane and in the vesicles of type-A cells of the synovial lining, and were observed in macrophages in the sublining layer. These results reveal that iNOS and IL-1β localize to the synovial membrane of the rat TMJ under physiological conditions. Therefore, it is likely that autocrine/paracrine effects of IL-1 β induce NO generation by iNOS via the IL-1R in type-A cells. It is considered that cytokine-induced NO may play an important role in the physiological maintenance, e.g. self-protection, by synovial lining cells of the synovial membrane in the TMJ.
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  • Immunocytochemical and Biochemical Studies of Glutathione-Peroxidase (GSH-PO) in Rat Gastric Parietal Cells
    Norio Kimura, Takashi Miwa
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    Subject area:  
    2002 Volume 35 Issue 1 Pages 23-31
    Published: 2002
    Released on J-STAGE: April 03, 2002
    DOIhttps://doi.org/10.1267/ahc.35.23
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    Histamine as an acid secretagogue and H2-blocker (roxatidine acetate hydrochloride) and a proton pump inhibitor (Omeprazole) as acid secretion inhibitors, in single and repeated doses, were administered to investigate associated lipid peroxidation in rat gastric parietal cells. Methods used included immunohistocytochemical localization and biochemical enzyme activity of glutathione-peroxidase, an enzyme that effectively reduces lipid peroxides. No changes in lipid peroxidation, in response to the H2-blocker, were observed, however its accumulation in the vicinity of vacuole-like structures was specific to proton pump inhibition. This finding suggests that lipid peroxidation occurs in local parietal cells.
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  • Immunohistochemical Analysis of Copper Chaperone for Superoxide Dismutase in Mouse Central Nervous System; a Comparative Study with Paraffin and Frozen Section
    Hisae Sumi-Akamaru, Harutoshi Fujimura, Shinsuke Kato, Satoshi Takayas ...
    Article type:  
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    2002 Volume 35 Issue 1 Pages 33-38
    Published: 2002
    Released on J-STAGE: April 03, 2002
    DOIhttps://doi.org/10.1267/ahc.35.33
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    To examine the immunohistochemical localization of copper chaperone for superoxide dismutase (CCS) in the mouse central nervous system (CNS), we produced an affinity purified polyclonal antibody against mouse CCS and compared paraffin sections with non-fixed or fixed frozen sections. Pyramidal neurons, Purkinje cells and anterior horn cells were intensely stained. The immunoreactivity was also present in other neurons throughout the CNS. The staining pattern with paraffin sections was similar to that with non-fixed and fixed frozen sections, indicating that the antibody could be used for the immunohistochemical investigations including localization of CCS in the motor neurons of familial amyotrophic lateral sclerosis model mice using permanent paraffin blocks.
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