ACTA HISTOCHEMICA ET CYTOCHEMICA
Online ISSN : 1347-5800
Print ISSN : 0044-5991
ISSN-L : 0044-5991
36 巻, 3 号
選択された号の論文の6件中1~6を表示しています
REVIEW
  • Masanori Yasuda, Masaru Murakami, Toshinari Muramatsu, Johbu Itoh, Kao ...
    原稿種別:  
    専門分野:  
    2003 年 36 巻 3 号 p. 185-192
    発行日: 2003年
    公開日: 2003/08/28
    ジャーナル フリー
    Expression of type-1 carbohydrate antigens (CBAs), DU-PAN-2 and CA19-9, was strongly dependent on hormonal regulation during the menstrual cycle in the endometrium. DU-PAN-2 expression was more frequent than that of CA19-9 in endometrial adenocarcinomas (EMACs), with no correlation to menopausal status. The relationship between immunohistochemical profiles and serum values was statistically proven to be closer for DU-PAN-2 than for CA19-9. Although during staging-up the serological positive ratios distinctly increased for both DU-PAN-2 and CA19-9, the serum DU-PAN-2 values tended to decrease according to histological grading-up and the reverse was true for CA19-9.
    In the analysis of variable thyroid diseases, from the specific and frequent expression of DU-PAN-2 in papillary carcinomas, it was suggested that DU-PAN-2 would be a useful immunohistochemical marker for distinguishing papillary carcinomas from follicular tumors. For testicular germ cell tumors (TGCTs), it could be emphasized that embryonal carcinomas were rather characteristic of extensive DU-PAN-2 expression. Particularly in CA19-9-negative cases, a combined analysis would be helpful in confirming the histologic diagnosis of embryonal carcinoma.
    The biological significance of characteristic DU-PAN-2 expression as a tumor marker in the above tumors remains to be clarified. The difference in activity of the specific glycosyltransferases, α-2-3-sialyltransferase and α-1-4-fucosyltransferase, may result in the variable expression profiles of these antigens.
  • Hideo Tanaka, Tetsuro Takamatsu
    原稿種別:  
    専門分野:  
    2003 年 36 巻 3 号 p. 193-204
    発行日: 2003年
    公開日: 2003/08/28
    ジャーナル フリー
    Ca2+ ions play pivotal roles in the excitation and contraction-relaxation processes of heart muscle cells. The advent of digital-imaging techniques, especially confocal laser scanning microscopy, as well as Ca2+-sensitive fluorescent dyes, has contributed substantially to the precise understanding of the spatiotemporal aspects of the intracellular Ca2+ dynamics in cardiomyocytes. In this review article, we review the progress in the cytochemical and histochemical researches on the intracellular Ca2+ concentration ([Ca2+]i) of the heart. Recent imaging technologies have revealed that subcellular [Ca2+]i dynamics are spatiotemporally heterogeneous under certain pathological conditions. We focus on the Ca2+ waves, i.e., representative of abnormal [Ca2+]i dynamics that emerge under [Ca2+] i-overloaded conditions, analyzed in preparations ranging from the enzymatically isolated myocytes to the whole heart. The pathophysiological significance of Ca2+ waves in the heart is also discussed.
REGULAR ARTICLE
  • Nobuyuki Susumu, Hayato Kawakami, Daisuke Aoki, Nao Suzuki, Atsushi Su ...
    原稿種別:  
    専門分野:  
    2003 年 36 巻 3 号 p. 205-214
    発行日: 2003年
    公開日: 2003/08/28
    ジャーナル フリー
    Frozen or resin sections of normal human endometrium, endometrial adenocarcinoma, and ovarian adenocarcinoma were examined with two monoclonal antibodies for galactosyltransferase associated with tumor (GAT) by immunofluorescence and immunoelectron microscopy. One antibody (MAb8628) stained the trans-cisternae of the Golgi apparatus, the trans-Golgi network, and the intracytoplasmic vesicles more intensely in cancer cells than in normal endometrial glandular cells. The other antibody (MAb8513) stained intracytoplasmic vesicles more intensely in cancer cells than in normal cells. MAb8513 also showed moderate staining of the trans-cisternae of the Golgi apparatus and trans-Golgi network in cancer cells, but only faint staining of these structures in normal glandular cells, indicating that GAT was overexpressed in the cancer cells. An immunofluorescence study using serial semithin cryosections (1 μm) demonstrated that the staining pattern of each antibody was different inside a single cancer cell, conforming to the two patterns mentioned above. The reason for this difference in staining remains unclear. GAT is a soluble form of β1,4-galactosyltransferase, so a difference in the cleavage site of the membrane-bound peptide may cause changes in immunoreactivity after its conversion to the soluble form (i.e., GAT) in endometrial or ovarian adenocarcinoma cells.
  • Takayuki Kondo, Ikuo Tooyama
    原稿種別:  
    専門分野:  
    2003 年 36 巻 3 号 p. 215-220
    発行日: 2003年
    公開日: 2003/08/28
    ジャーナル フリー
    Alpha2-macroglobulin (A2M), a pan-proteinase inhibitor, accumulates in senile plaques of Alzheimer disease. In this study, we investigated the immunohistochemical localization of A2M in the brain of PS/APP mice. In control mice, A2M immunoreactivity was restricted to the vessels of brain parenchyma. In PS/APP mice, A2M deposition occurred in the brain parenchyma. A2M-positive deposits appeared after three months of age when senile plaque formation began to occur. The number of A2M-positive deposits increased with age. Double-staining for A2M and thioflavin S revealed that A2M accumulated in a subpopulation of thioflavin S-positive fibrillar type of amyloid beta deposits. These results indicate that A2M is implicated in the formation of fibrillar type senile plaques in PS/APP mice.
  • Kiyokazu Kametani, Kayo Suzuki, Tetsuji Nagata
    原稿種別:  
    専門分野:  
    2003 年 36 巻 3 号 p. 221-230
    発行日: 2003年
    公開日: 2003/08/28
    ジャーナル フリー
    Elemental analysis on Al was carried out by energy dispersive X-ray (EDX) microanalysis using high accelerating voltage at 300 kV with high permeability in thick samples at 1 μm thickness obtained from 27 ddY mice administered with Al for 1 to 17 weeks. The quantities of Al detected in the lysosomes of the convoluted tubule cells of the kidney did not relate to the Al administration period. A significant difference in the quantity of Al between the administration group and the non-administration group was found by stochastical analysis with two-way factorial analysis of variance followed by Fisher's PLSD for multi-comparison. However, the quantities of Al detected in the nuclei of the proximal convoluted tubule cells of the kidney suggested a correlation with the Al administration period. EDX microanalysis using thick sections and high accelerating voltage was useful in detecting the Al accumulated in the lysosomes and nuclei of the kidney cells. The EDX microanalysis was able to detect Al accumulated in the lysosomes and clarify the transitional changes of the quantities of Al detected during a short-term excessive Al administration.
  • Yan Jin, Fumihiko Suwa, Xin Nie, George L. Tipoe, Yoshitaka Tamada, Yi ...
    原稿種別:  
    専門分野:  
    2003 年 36 巻 3 号 p. 231-237
    発行日: 2003年
    公開日: 2003/08/28
    ジャーナル フリー
    Transection of the rat facial nerve (FN) leads to a series of retrograde changes within the facial nuclei, which has been called the axon reaction. This reaction includes the rapid activation of both astrocytes and microglia and a change in the gene expression in axotomized motoneurons. In the present study, we investigated the expression of the transforming growth factor-β (TGF-β) superfamily member [bone morphogenetic protein (BMP)-2, 4, 6 and TGF-β1] in the axotomized facial nuclei using in situ hybridization. The aim of the present study was to clarify whether the BMP-2, 4, 6 and TGF-β1 are related to the regenerative and degenerative processes in the axotomized facial nuclei. The results showed that BMP-2 and TGF-β1 mRNA increased gradually after axotomy, and reached the maximal values on day 7 post injury. BMP-6 was expressed abundantly in the facial motoneurons, and no obvious differences were shown in the gene expression after transection of FN. However, no significant signals for BMP-4 in motoneuron were detected at any time point. These results revealed that some members of the TGF-β superfamily such as BMP-2 and TGF-β1, might be involved in glial activation and motoneuron survival, and might be potential neurotrophic factors of FN.
feedback
Top