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Volume 36 (2003)
- Issue 6 Pages 495-
- Issue 5 Pages 415-
- Issue 4 Pages 249-
- Issue 3 Pages 185-
- Issue 2 Pages 99-
- Issue 1 Pages 1-
Volume 36, Issue 6
Displaying 1-4 of 4 articles from this issue
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REVIEW
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Masanori Murakoshi, R. Yoshiyuki Osamura, Keiichi WatanabeArticle type:
Subject area:
2003 Volume 36 Issue 6 Pages 495-500
Published: 2003
Released on J-STAGE: January 30, 2004
JOURNAL FREE ACCESSThe effect of testosterone and estradiol on the prostate of castrated rats (rat prostatic hyperplasia model after combined treatment with testosterone and estradiol) were investigated by histopathologically and immunohistochemical procedures. The castrated rats were treated for 6 weeks with 1) testosterone 1 mg/animal and 2) testosterone 1 mg/animal plus 17beta-estradiol (estradiol) 10 μg/animal. Histopathologically, glandular hyperplasia of the ventral prostate and glandular hyperplasia with fibromuscular stromal proliferation in the dorsolateral prostate, was clearly observed. Immunohistochemical localization of glutathione peroxidase (GSH-PO) which effectively reduces the lipid peroxides, was predominantly observed in the glandular epithelial cells of the ventral prostate. The intensity of GSH-PO staining in the glandular epithelial cells was remarkably decreased after castration, and that it was clearly recovered by testosterone or testosterone plus estradiol treatment to the castrated rats. In contrast, GSH-PO was clearly observed in the glandular epithelial cells of the dorsolateral prostate following testosterone alone or testos-terone plus estradiol. Androgen receptor (AR) is mainly localized in nuclei. In the dorsolateral prostate, AR was also detected in the nuclei of the proliferated stromal fibromuscular cells. Furthermore, immunodetectable AR rapidly declined after androgen withdrawal and returned to intact levels of staining intensity after androgen replacement. These findings strongly suggest that expressions of GSH-PO and AR in the prostate are testosterone-dependent. It is concluded that immunohistochemical analysis, using GSH-PO and AR, may be a useful method for prediction of the effects of androgen action on the rat prostate.
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Akira Matsuno, Johbu Itoh, Susumu Takekoshi, Tadashi Nagashima, R. Yos ...Article type:
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2003 Volume 36 Issue 6 Pages 501-506
Published: 2003
Released on J-STAGE: January 30, 2004
JOURNAL FREE ACCESSThe rab3 protein is involved in the exocytosis of synaptic vesicles and secretory granules in the central nervous system and anterior pituitary cells. We have previously elucidated the role of rab3B in growth hormone (GH) secretion and the mutual relationship between rab3B and soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins. Confocal laser scanning microscopic observation of double immunohistochemical stainings for rab3B and GH revealed an increase of immunoreactivity for rab3B in GHRH-treated rats and a decrease of immunoreactivity for rab3B in SRIF-treated rats. Moreover, confocal laser scanning microscopic observation of double immunohistochemical stainings of SNAP-25, syntaxin and rab3B revealed co-localization of rab3B and the SNARE proteins in GHRH-treated rats, and dissociation in SRIF-treated rats. These results suggest that rab3B plays an important role in GH secretion from the anterior pituitary cells, and that SNAP-25 and syntaxin act alongside rab3B in the functional regulation of GH secretion. Other reports have indicated that rho and rab proteins such as rab8B and rab27B are involved in the intracellular transport and secretion of pituitary hormones. Functional analyses of rab and rho protein families and the SNARE system are required to elucidate the precise mechanisms of the intracellular transport and secretion of pituitary hormones.
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NOTE
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Yoshinari Isobe, Naoki Isobe, Mie Kanato, Izumi MaruyamaArticle type:
Subject area:
2003 Volume 36 Issue 6 Pages 507-509
Published: 2003
Released on J-STAGE: January 30, 2004
JOURNAL FREE ACCESSWe applied a combined peroxidase and Paragon staining to epoxy-embedded tissue sections for polychrome stain. In contrast to monochrome toluidine staining, our method facilitated identification of a variety of cells in the tissue and their spatial relationship.
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Yutaka TsutsumiArticle type:
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2003 Volume 36 Issue 6 Pages 511-514
Published: 2003
Released on J-STAGE: January 30, 2004
JOURNAL FREE ACCESSFour unstained glass slides prepared from three cases autopsied by Dr. Thomas Hodgkin himself over 170 years ago were obtained during a visit to Gordon Museum, Guy's Hospital Medical School, London, UK. Hematoxylin and eosin staining confirmed the well-preserved spleen and autolytic liver to be massively infiltrated by Reed-Sternberg (R-S) cells in fibronodular background, and the two lymph nodes to have numerous R-S cells in one case and features of non-Hodgkin's lymphoma in the other. After bleaching the dyes, sections were transferred to silane-coated glass slides, and for the liver and spleen, the sections were divided into two. The labeled streptavidin biotinylated peroxidase method was employed for detecting CD15, CD30 and LMP-1, and the in situ hybridization technique for EBER-1 (Epstein-Barr virus-related small nuclear RNA). For the liver and spleen, EBER-1 was re-stained using sections negative for LMP-1. CD15 was detected on R-S cells in the liver. CD30 and LMP-1 were undetectable. EBER-1 was demonstrated in the nuclei of R-S cells in the lymph node, liver and spleen. The lymph node of non-Hodgkin's lymphoma was negative for EBER-1. The present study confirmed the diagnosis of Hodgkin's lymphoma and Epstein-Barr viral infection in R-S cells in archival samples from two of the three original cases autopsied by Dr. Thomas Hodgkin.
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