巻号一覧
38 巻 (2005)
- 6 号 p. 347-
- 5 号 p. 295-
- 4 号 p. 253-
- 3 号 p. 185-
- 2 号 p. 87-
- 1 号 p. 1-
38 巻, 4 号
選択された号の論文の6件中1～6を表示しています
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REVIEW
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Akira Matsuno, Johbu Itoh, Susumu Takekoshi, Tadashi Nagashima, R. Yos ...2005 年 38 巻 4 号 p. 253-256
発行日: 2005年
公開日: 2005/09/23
ジャーナル フリーSemiconductor nanocrystals (Quantum dots, Qdots) have recently been used in biological research, since they do not fade upon exposure to light, enabling us to obtain multicolor imaging due to a narrow emission peak that can be excited via a single wavelength of light. Utilizing the advantages of Qdot and confocal laser scanning microscopy (CLSM), we can obtain three-dimensional images of the intracellular localization of growth hormone and mRNA in a pituitary cell. The previous method of combined in situ hybridization (ISH) and immunohistochemistry (IHC) under electron microscopy has sufficient ultrastructural resolution, but can provide only two-dimensional images. By contrast, ISH and IHC using Qdots and CLSM can optimally illustrate the simultaneous relationship between protein and mRNA in three dimensions. Such an approach enables us to better visualize functional images of proteins in relation to mRNA synthesis and localization.
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Hideo Tanaka, Tetsu Hamamoto, Tetsuro Takamatsu2005 年 38 巻 4 号 p. 257-265
発行日: 2005年
公開日: 2005/09/23
ジャーナル フリーPurkinje fibers are an essential element of the conduction system that provides for coordinated ventricular contraction of the heart. Although classic studies established structure and electrical activities of Purkinje fibers, an integrated understanding of the Purkinje-ventricular interconnection within the working heart remains to be had. In this review article we will briefly overview the gross anatomy and cytological characteristics of Purkinje fibers and their transition to the ventricular muscle, and we will discuss functional and morphological aspects of the Purkinje-ventricular interconnection from the perspectives of electrophysiology, distribution of gap junctions, and intracellular Ca2+ ([Ca2+] i) dynamics. Furthermore, the potential usefulness of the recently developed optical voltage-mapping and in situ real-time confocal [Ca2+]i imaging techniques is discussed as an integrated approach to the pathophysiological investigation of the Purkinje-ventricular interconnection in the heart.
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TECHNICAL ADVANCEMENT
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Naoki Ogane, Masanori Yasuda, Hiroyuki Hayashi, Yoichi Kameda, Takeo M ...2005 年 38 巻 4 号 p. 267-271
発行日: 2005年
公開日: 2005/09/23
ジャーナル フリーImmunohistochemistry is a useful diagnostic procedure in routine pathological practice including cytology. However, when occasional discrepancy between cytological immunostaining and histological immunostaining in the intracellular localization of a positive reaction is encountered, we tend to think that the discrepancy is due to differences in the fixation agents and staining processes for these two methods. As a demonstrable example of such a discrepancy, glucose transporter-1 (GLUT-1) staining profiles in cytological specimen and histological specimen are presented. GLUT-1 overexpression is usually considered to be predominant on the cell membrane, based on the function of GLUT-1 involved in the effective transportation of glucose, but GLUT-1 is observed in the cytoplasm more often than expected. Especially in cytological specimens, localization of GLUT-1 expression is occasionally difficult to recognize because of ill-defined cell cluster structure and marked stratification. The interpretation may result in a pitfall: the staining pattern basically differs from that in the histological specimen. Confocal laser scanning microscopy (CLSM) is easily applied to usual cytological immunostaining and histological immunostaining colorized by diaminobenzidine as well as Papanicolau-stained and hematoxylin & eosin-stained specimens without specific or tedious pretreatment. Observation of immunohistochemical specimens using CLSM is very helpful in accurate interpretation of the immunolocalization especially because the cytoplasm-predominant expression of the immunoreaction often assumes a seemingly membrane-predominant pattern in cytological specimens.
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REGULAR ARTICLE
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Yutaka Endo, Keita Harada, Naoji Fujishiro, Issei Imanaga, Koichi Ogaw ...2005 年 38 巻 4 号 p. 273-282
発行日: 2005年
公開日: 2005/09/23
ジャーナル フリーThe stimulation of muscarinic receptors induces the activation of nonselective cation (NS) channels, in an internal ATP-dependent manner, in guinea-pig adrenal chromaffin cells. Therefore, we attempted to determine with fluorescent techniques in which part of the cell membrane muscarinic receptors (R) and NS channels were localized. The m4AChR was immunodetected in the adrenal medulla and not in the cortex. A three-dimensional analysis of dissociated chromaffin cells revealed that the cell comprised a nuclear portion that contained mainly the nucleus and a nonnuclear portion. The m4-like immunoreactivity (IR) was mainly distributed in the membrane area in the nuclear portion, and the majority of m4-like IR was closely associated with nicotinic AChR-like IR in double stainings. The intracellular concentration of Na+, as measured with SBFI dye, increased in response to muscarine, and this increase was larger in the nuclear portion than in the non-nuclear portion. We thus concluded that m4 receptors and probably NS channels are distributed in the plasma membrane near the nucleus.
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RAPID COMMUNICATION
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Kotaro Horiguchi, Jun-ichiro Naito, Tomoko Fujisawa, Satoshi Ohashi, M ...2005 年 38 巻 4 号 p. 283-287
発行日: 2005年
公開日: 2005/09/23
ジャーナル フリーEctopic gene expression of prolactin (PRL) has been reported in different tissues among several species. Recently, lymphocytes have also been shown to produce PRL, which is considered to be involved in the immunoregulatory system. However, when and where PRL synthesis occurs in lymphocytes remain unknown.
In the present study, we attempted to examine the existence of PRL producing cells in the mouse thymus and spleen of immune tissues. We performed double fluorescence immunohistochemistry (DFIH) for mouse PRL (mPRL) and mouse CD4 that was specifically produced in T cells, and for mPRL and mouse CD25 or CD44, which specify the CD4+ precursor cells in the early stage of T cell differentiation in the thymus. Similarly, we performed DFIH for mPRL and mouse IL-4 or IFN-γ which are specifically produced in helper T cells of the mouse spleen. The immunoreactive substance for mPRL was co-localized in some CD4 or CD44 immunopositive cells in the thymus and some IL-4 or IFN-γ producing cells in the spleen. In conclusion, we found that mPRL is synthesized at the early stage of T cell differentiation.
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Erratum
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2005 年 38 巻 4 号 p. 289
発行日: 2005年
公開日: 2005/09/23
ジャーナル フリーWe hope to change a last word "by us" of Introduction into by "Kitamura et al. 1991", and then add "Kitamura, H., Takita, K. and Nakamura, M. (1991) Improvement of a physical developer devoid of gum Arabic for immunohistochemical use. Acta Histochem. Cytochem. 24(5); 521" in References.
（誤）by us
（正）by Kitamura et al. 1991抄録全体を表示PDF形式でダウンロード (23K)
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