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Volume 4 (1971)
- Issue 4 Pages 191-
- Issue 3 Pages 111-
- Issue 2 Pages 65-
- Issue 1 Pages 1-
Volume 4, Issue 3
Displaying 1-7 of 7 articles from this issue
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KOICHI OGA1971 Volume 4 Issue 3 Pages 111-125
Published: 1971
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSChE activity in the canine heart was studied by light and electron microscopy by Karnovsky method.
1. Sinus and atrioventricular nodes were stained most darkly in which numerous autonomic nerves and transitional cells showed most intense ChE activity.
Atrioventricular bundle and the atrial septal muscle near the A-V node also showed intense staining. These atrial septal muscles, which show strong ChE activity, were identical to the internodal tracts and bypass tracts (James et al., 1966). The atrial and ventricular muscles showed very low ChE activity. The precipitation of reaction products grew larger in the specialized muscle cells, but those in ordinary cardiac muscle were small and fine.
2. In P-cells, deposits of the reaction product were not great because sarcoplasmic reticulum and myofilaments were scarce.
3. Papillary muscle showed the interesting figure of subendocardial dark ring after incubation in Karnovsky incubation medium. This thin layer is suspected to be a part of the conduction system.
4. Intracardiac ganglia were often seen near the sinus node and sometimes at the peripheral region of the sinus node. All the ganglion cells studied were intensely positive for ChE activity.
5. Although small cored vesicles in nerve terminals were rarely demonstrated in the present histochemical study, all the terminals which included the small cored vesicles were ChE negative.View full abstractDownload PDF (16451K) -
SETSUYA FUJITA, SATORU YOSHIDA, MASARU FUKUDA1971 Volume 4 Issue 3 Pages 126-136
Published: 1971
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSIn absorption cytophotometry, instrumental and optic errors are important. Although most of them have been studied extensively and practically solved, the so far unrecognised problem of non-specific loss of light arises in the measurements on biological materials especially on sections of brain tissues. Unstained objects scatter significant amount of light at various wave-lengths. Cytophotometry on DNA content of the cells in Feulgen stained material is affected significantly by this factor. In Feulgen stained materials, such non-specific light loss of the light is detected by scanning with a light spot at wave-length of 450 or 650mμ. In sections of rat cerebellum, the non-specific light loss amounts to a considerable quantity and is difficult to eliminate. By preparing smears directly from the brain tissue, the authors succeeded in minimizing the effect of the non-specific light loss. Nuclei of Purkinje cells and inner granule cells in the smears are studied for their Feulgen dye content by total scanning method. The data obtained on smeared cells clearly indicate that both types of cells are in the same class of DNA content.View full abstractDownload PDF (4030K) -
ENZYME-MORPHOLOGICAL AND ULTRASTRUCTURAL OBSERVATIONTSUTOMU KOIDE, KENSUKE BABA, KEIICHI WATANABE, MINAKO IKEDA, YUKIO SHI ...1971 Volume 4 Issue 3 Pages 137-152
Published: 1971
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSIn order to see the influence of mitochondrial damages on the cell, timelapsed morphological changes in HeLa cells after staining supravitally with various concentrations of Janus Green B were observed. For light microscopy, NADH dehydrogenase activity employing tetranitroblue tetrazolium was used for the mitochondrial marker and acid phosphatase activity by azo-dye method for the lysosomal marker. Individual specimens were stained with both methods successively. Ultrathin sections prepared from the monolayer cells on cover-slips were examined by an electron-microscope with or without prior histochemical reaction for acid phosphatase.
At concentrations of Janus Green B lower than 2.5×10-6M, no changes were observed in any organelles but mitochondria. And the concentration of Janus Green B around 1×10-6M was confirmed to be appropriate for the selective injury of the mitochondria with combination of Janus Green B and ruby laser light, results of which had been reported previously. However, at least from the morphological view points, Janus Green B itself was found to be selectively injurious to mitochondria at lower concentrations. At the concentration around 2.0×10-5M, which was used conventionally for observation of mitochondria, changes in mitochondria were observed immediately after staining for 8 to 15 minutes. With 10-3 M Janus Green B, cells were fixed on the glass surface and the enzyme activities were entirely lost. Cellular degenerative process produced by Janus Green B was discussed, particularly placing emphasis on the mitochondrial changes and on the role of lysosome.View full abstractDownload PDF (20506K) -
TAKAO KAWASHIMA, TAKURO OGATA, KUNIO OKAJIMA1971 Volume 4 Issue 3 Pages 153-160
Published: 1971
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSCorrelation between histochemical enzyme classification and prognosis of gastric carcinoma was studied. Prognosis and selection of the operative procedure for stomach cancer is helped by a relatively simple histochemical enzyme study of surgical specimens.View full abstractDownload PDF (640K) -
YASUHIRO AGO, KAZUO OGAWA1971 Volume 4 Issue 3 Pages 161-165
Published: 1971
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSAs an extension of our studies on the copper ferrocyanide method for the ultracytochemical demonstration of oxidoreductase activity, the method for the detection of NADPH-ferricyanide reductase activity was developed. The incubation medium consists of 20mg of NADPH, 13.0ml of 0.1M phosphate buffer, pH 7.0, 1.3ml of 0.1M sodium citrate, 2.0ml of 30mM copper sulfate, 1.7ml of double distilled water, 2.0ml of 5mM potassium ferricyanide and 3.0g of sucrose. It was observed in the preliminary experiment that potassium sodium tartrate caused more diffusion of the final reaction products than sodium citrate.
Fresh tissues from brain, liver, heart and kidney of normal adult rats were used. Formaldelyde inhibited the enzymatic activity completely. Incubation with medium was carried out for 30 minutes at 37°C under aerobic and anaerobic conditions. After the incubation specimens were fixed in 1% osmium tetroxide and processed for electron microscopy.
The NADPH-ferricyanide reductase activity was extremely low in all tissues examined. In the enzymatically positive tissues the activity was observed solely in mitochondria. No activity was demonstrated in any element of the endoplasmic reticulum. The enzymatic activity in specimens incubated under anaerobic conditions was slightly higher in terms of the number of enzymatically positive mitochondria and the rate of positive reaction in each mitochondrion than that incubated under aerobic conditions.
In mitochondria the reaction product was observed in the membrane system including the outer membrane, the intracristal space and the outer space. In some areas, the activity in the outer membrane was quite evident. No activity was observed in the mitochondrial matrix.View full abstractDownload PDF (3446K) -
SUSUMU TOMONAGA, AKITO NOMURA, KAZUHIKO AWAYA1971 Volume 4 Issue 3 Pages 166-172
Published: 1971
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSFor quantitative determination of Feulgen-DNA in mouse lymphocytes three hydrolysis methods were examined.
With 5N HCl hydrolysis at 25°C, there was found a long plateau of optimum hydrolysis over 30 minutes or more, while the period of optimum hydrolysis in the conventional hydrolysis at 60°C in 1N HCl was a few minutes, and that in 60°C 5N HCl was a few seconds.
Proportionality of DNA content between spermatids and lymphocytes was well preserved over the long hydrolysis time in 25°C 5N HCl hydrolysis.
Therefore, it was concluded that 25°C, 5N HCl hydrolysis was preferable to the conventional Feulgen hydrolysis performed at 60°C with 1N HCl.View full abstractDownload PDF (630K) -
P. P. SOOD, H. B. TEWARI1971 Volume 4 Issue 3 Pages 173-185
Published: 1971
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe present contribution, detailing the distribution of various phosphatases (alkaline and acid phosphatases, 5-nucleotidase and ATPase) in the accessory olfactory bulbs of rat, mouse and frog and their consideration in a comparative way, brings up some interesting features. The most important among them relate to the enzymatic equipment of the fibrous bundles which seem unique to mammals and is not seen in frog. The other significant observation is the presence of ATPase along the entire lengths of the fibers constituting the superficial fibrous layer of the olfactory bulbs of mammals and frog. Further, the granular layer is negative for 5-nucleotidase in rat and mouse but positive in frog. An attempt has been made to correlate the various patterns of enzymatic localization with the functional activity of the various constituents in the accessory olfactory bulbs of rat, mouse and frog.View full abstractDownload PDF (14206K)
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